France-BioImaging, with its partner the GDR IMABIO, organizes the 4th edition of the FBI-AT: an advanced microscopy workshop to be held in Paris from November 21st to 25th, 2022.

The aim of this France-BioImaging-Advanced Training is to train microscopy users on the most advanced imaging techniques that will allow them to perform molecular studies at the cellular level as well as in thick samples. In particular, recent developments on fluorescent probes will be highlighted. The workshop will benefit from state-of-the-art equipment available on several of the Parisian Node Imaging facilities.

This year’s edition will have plenary lectures given by experts in the microscopy development field. These seminars will be advertised as a series and will be broadcasted for a large audience.  In addition, specific techniques will be introduced.

Hands-on practicals will train attendants on these techniques every afternoon in different sites in Paris including Institut Curie, Institut Pasteur, Institut Cochin, Institut Jacques Monod, Institut de Psychiatrie et Neurosciences de Paris and ENS Paris. Access to this part of training will be restricted to selected and registered trainees.

To guaranty access to set-ups and proper training, each practical session will host only 3-4 persons. The sessions will be run in parallel.

Apply now, attendance will be limited to 25 participants! 

FBI-AT is ideal for researchers with a basic training in microscopy willing to become familiar with advanced techniques to answer their specific biological questions, or to be exposed to new developments that will allow them to tackle new questions in their project. We will consider applications from early career researchers (PhD students, post-docs), technical staff members and more senior scientists.

AT A GLANCE

The workshop contains plenary lectures and specific training sessions. Plenary lectures will be on hybrid mode and largely open.

Invited Speakers

Emmanuel Beaurepaire

Giulia Bertolin

Joerg Bewersdorf

Peter Dedecker

Claire Deo

Marie Erard

Ricardo Henriques

Christophe Leterrier

Sandrine Lévêque-Fort

Gustavo Quintas

Gaelle Recher

Jean-Baptiste Sibarita

Lothar Schermelleh

Practicals on

  • Combining micro UV-irradiation and Single Particle Tracking in living cells
  • SMLM multi-color: from sample preparation to quantification
  • FRET-based molecular tension sensors and FLIM
  • Imaging of cellular ultrastructures with expansion microscopy
  • SIM, STED or STORM ? : from sample prep to 3D imaging
  • 3D STED : Comparing flat cells vs thick samples
  • Culturing and imaging multicolour 3D live brain organoïds
  • Combining fast live 3D imaging with Z resolution preservation
  • Light sources for optogenetics
  • Non-classical genetically modified fluorescent probes for biological imaging
  • Imaging biological structures in 3D using double helix-STORM and 3D-SIM

Organizers

Florence Niedergang, Lydia Danglot, Chloe Guedj, Mickael Lelek, Pierre Bourdoncle, Audrey Salles, Xavier Baudin, Nicolas Borghi, René-Marc Mege, David Geny, Ludovic Jullien

Poster

Sponsors

Les inscriptions pour la seconde édition du TED ImaBio lundi 2 mai à Montpellier sont ouvertes.

Le GDR ImaBio vous propose de venir decouvrir les dernières innovations des partenaires industriels du GDR , et pour les etudiants un focus sur les métiers du domaine.

Programme

The 7th edition of OPTOGEN will bring developers and end-users in the field of Optogenetics and Neurophotonics together. It will be a great occasion to meet in person for stimulating discussions and to reignite collaborations.

Registration and abstract submission are open.

Early registration will close on April 15th.

Thanks to the support of the IHU Foresight and of the sponsors, inscription is free for students registered before April 15th and some travel grants will be available.

Euro-BioImaging is organizing a third online User Forum on Tuesday, April 5, 2022 from 14:00-17:00 CEST. The topic is “At the Forefront of Neuroscience.”

This event will highlight the importance of cutting-edge imaging technologies in support of brain research and showcase the specific expertise available at our Nodes across Europe through case studies presented in tandem with the research community. 

In addition, keynote presentations from Hervé Boutin, University of Manchester, and Selma Ding, Max Planck Institute for Animal Behavior, will further reveal the potential of biological and biomedical imaging technologies to boost brain research. 


Register here: https://us02web.zoom.us/meeting/register/tZ0ucuytpj8iE92A-JjvPbvpNnmTF4Z7iRaC

More information: https://www.eurobioimaging.eu/news/join-us-for-the-third-euro-bioimaging-user-forum-at-the-forefront-of-neuroscience/

Programme

Deadline: April 15th, 2022

The three national infrastructures ProFi, France-BioImaging and FRISBI along with the GIS IBiSA are pleased to announce a call for a funded access to IBiSA-labelled facilities.

Our aim is to promote IBiSA facilities networking through interdisciplinary research projects.

Applications should request access to at least two different IBiSA facilities from two disciplines (structural biology, Biological imaging and proteomics). The call is open to any academic laboratory.

The amount of the financial support will be up to 5000 € per application to cover facility costs.

Applications should be submitted to: Call-IBISA-FBI-FRISBI-PROFI@i2bc.paris-saclay.fr
using the template document https://mycore.core-cloud.net/index.php/s/VNLU2jgfcP4o0NN


The deadline for this inter-infrastructure access call is 15 April 2022.

All submitted proposals will be peer-reviewed by independent experts and the final funding will be approved by a committee comprising 2 representatives from each infrastructure as well as representatives
from GIS IBISA. We advise the project PI to contact the chosen facilities in order to set-up the optimal experimental design.

Call description

The France-BioImaging LSFM workgroup is pleased to announce an INSERM workshop on Light-Sheet Fluorescence Microscopy imaging technics. 

This workshop will be divided in two parts:

  • A first theoretical part in Bordeaux from the 16th to the 18th of May 2022 which will cover basic principles, applications and challenges of LSFM imaging through seminars,
  • A second practical part along June 2022 where you will have the possibility to choose the set-up on which you want to be trained amongst many systems available within the France-BioImaging community.

If you want to discover, learn and/or deepen your knowledge about this vast and powerful family of 3D imaging technics, this training is for you!


You will find all the information (program, registration details, venue and accommodations, …) about this event on the following poster and website (Atelier 268): https://evenium-site.com/site/atelier-de-l-inserm-268

REGISTRATION DEADLINE: March 4, 2022

Programme

Poster

Understanding how development is coordinated in multiple tissues and gives rise to fully functional organs or whole organisms necessitates microscopy tools. Over the last decade numerous advances have been made in live-imaging, enabling high resolution imaging of whole organisms at cellular resolution. Yet, these advances mainly rely on mounting the specimen in agarose or aqueous solutions, precluding imaging of organisms whose oxygen uptake depends on ventilation.

Engineers from the Institut Curie and Institut Jacques Monod implemented a multi-view multi-scale microscopy strategy based on confocal spinning disk microscopy, called Multi-View confocal microScopy (MuViScopy).

MuViScopy enables live-imaging of multiple organs with cellular resolution using sample rotation and confocal imaging without the need of sample embedding. They illustrated the capacity of MuViScopy by live-imaging Drosophila melanogaster pupal development throughout metamorphosis, highlighting how internal organs are formed and multiple organ development is coordinated. They foresee that MuViScopy will open the path to better understand developmental processes at the whole organism scale in living systems that require gas exchange by ventilation.

3D fusion reconstruction of eight different angles at 10x magnification. Animation of a 3D reconstruction after fusion of images acquired from eight angles by the MuViScope of an Ecad:3xGFP Drosophila pupa at 28 hAPF with a 10x objective. The animation starts with a 180° rotation along the A-P axis and then sequentially shows the eight different angles from a top view: 0° (red), 45° (orange), 90° (yellow), 135° (green), 180° (light blue), 225° (dark blue), 270° (purple) and 315° (pink). Fusion was performed using Huygens Fuser (SVI) and 3D visualization with Imaris software. The acquisition parameters are detailed in Table S1. Scale bar: 200 μm.

Olivier Leroy, Eric van Leen, Philippe Girard, Aurélien Villedieu, Christian Hubert, Floris Bosveld, Yohanns Bellaïche, Olivier Renaud; Multi-view confocal microscopy enables multiple organ and whole organism live-imagingDevelopment 15 February 2022; 149 (4): dev199760. doi: https://doi-org.insb.bib.cnrs.fr/10.1242/dev.199760

The MuViScope was co-funded by France-BioImaging.

Le Réseau d’Imagerie Cellulaire Paris-Saclay organise en avril prochain une visioconférence de 2 heures portant sur le thème de l’imagerie des organoïdes.

Titre : Pipeline Organoïdes de A à Z : Fabrication, Transparisation, Acquisition et Analyse d’images.

Thématique : Le RIC Paris-Saclay vous propose une sorte de pipeline sur l’imagerie des modèles organoïdes qui vous permettra de mieux appréhender l’ensemble du processus de leurs fabrications, leurs modes de transparisation, l’analyse des images 3D associées à ce type d’échantillon épais, et pour finir, l’importance de l’étude de ces modèles chez l’homme pour développer de nouveaux outils thérapeutiques.

Date : 14 avril 2022
Horaire : 14h à 16h

Programme : Auto-organisation cellulaire en systèmes 3D par imageries optiques — Tissue clearing : Adapted protocols for small samples — Analyses d’images et reconstruction 3D — Applications en Recherche translationnelle (Organoïdes hépatiques humains).

Inscription gratuite et obligatoire sur www.ric-paris-saclay.fr

Programme

Affiche

The thematic institute of molecular and structural basis of life sciences of the French National Alliance for Life Sciences and Health (Aviesan) has identified cellular (cryo) electron microscopy as a priority area to be developed in the future. To better understand the functional behaviour of living organism, it becomes increasingly important to describe its structural organization from atomic scales to integrated, multi-component biological systems. Recent developments in imaging have drastically transformed our perception of the molecular organization and of the dynamic interactions of molecules in cellular systems.

That is why, they are organizing a 2-days meeting entitled “Symposium on recent developments in cellular electron microscopy”, and dedicated to the latest progresses in the field of cellular electron microscopy at the interface with fluorescent microscopy and structural biology. The meeting is built around 4 scientific sessions, namely volumetric imaging, cryo-electron tomography, correlative light and electron microscopy, and new technological developments. The meeting is for molecular and cellular biologists to have an overview of the latest results and concepts that develop at this interface.

Due to the sanitary situation, vaccination pass and face masks are mandatory to attend the meeting.

Download the provisional program

ORGANIZING COMMITTEE

Yves Gaudin, I2BC

Carine Giovannangeli, AVIESAN

Bruno Robert, CEA

Patrick Schultz, IGBMC

  • Deadline: March 4th, 2022
  • For users from a unit attached to the INSB who do not usually use Research Infrastructures’ services for their projects
  • For access to technologies and related expertise
  • For access to expertise in data analysis
  • The scientific project must be a project in the process of being finalized

The field of Life Sciences has undergone major developments over the past two decades. The change of scales, both in spatial and temporal resolution, and the integration of data from a wide variety of sources, as induced by the development of technologies, have revolutionized the exploration of life. These technologies call for expensive investments and specific know-how, carried out by highly qualified personnel, having led to the creation of common infrastructures such as research infrastructures (RIs) open to the entire scientific community.
The Institut des Sciences Biologiques (INSB) from Centre National de la Recherche Scientifique (CNRS) is launching a call for projects to fund full access to the national infrastructures in health and biology “INBS Access to national research infrastructures”. This call aims to encourage new teams to use the services offered by Research Infrastructures like France-BioImaging to help their research project. The objective is to demonstrate the significant impact of these services to improve the quality of their results (resolution, reproducibility, change of scale, etc.) or to help remove technological barriers.

Program description

Access to Research Infrastructures is open to the entire French and international scientific community, with costs covered by facility fee billing. The purpose of this program is to facilitate and finance access to these infrastructures in order to encourage teams who do not usually use the research infrastructures’ services to become RIs users, to validate the contribution that the infrastructures could make to their research topic, to remove barriers that impair the completion or finalization of an ongoing project.
As National Research Infrastructure, France-BioImaging is eligible and can host the applicant’s project on one of its facilities.
Several types of access can be supported by this call for projects:

  • Access to technologies and related expertise
  • Access to expertise in data analysis


In addition to the costs of access to infrastructure, travel/mission costs, as well as consumables that are not covered by the costs of access to infrastructure, may be covered.
The funding for each project will be in the range of 10 to 30 K€.

Instructions for submission

This is two-stage submission.
The first stage consists of a letter of intent, prepared by the scientific leader of the project, outlining the
project in which the application fits and highlighting the barriers that could be removed by access to IRs.
This letter of intent must also be signed by the director of the applicant’s unit. The identification of the
infrastructures to access is possible at this stage but not mandatory.
The deadline for submission of this letter is Friday 4th March 2022.

The second phase consists of a consultation between the INSB and the national Research Infrastructures. This will lead to putting the applicant in contact with the infrastructures that are able to meet the expressed needs, in order to evaluate the feasibility and to establish the budget of the final proposal. This stage will lead to the final submission of the project, signed by the heads of the involved IR(s), no later than Friday 8th April 2022.

Project eligibility and selection criteria

  • The scientific leader of the project should be in a unit attached to the INSB.
  • Access to the infrastructure must be fully implemented before 31/12/2022 (deadline for the use of funds)
  • The scientific project must be a project in the process of being finalized.
  • Access to infrastructure must unlock or accelerate the project. The start of a scientific project is not eligible for this call.
  • The letter of intent (maximum of three pages including figures) accompanied by a CV of the project leader (maximum of two pages) must be sent before Friday 4th March 2022 to the following address: insb.ain@cnrs.fr.

France-BioImaging is launching a call to have up to two new Nodes joining the infrastructure.

In 2022, France-BioImaging gathers 21 biological imaging facilities and 54 R&D teams specialized in biological imaging (see https://france-bioimaging.org/). It is structured in 6 local Nodes (Bordeaux, Bretagne-Loire, Ile-de-France-Sud, Marseille, Montpellier, Paris) and one transversal Node dedicated to bioimage informatics (IPDM). France-BioImaging is also the French node of Euro-BioImaging ERIC (see https://www.eurobioimaging.eu/).

The objectives of France-BioImaging are to promote the dissemination of the latest advances in all technologies and methods related to biological imaging, and their adoption by the users of the facilities that constitute the infrastructure.

In order to expand its technology and expertise portfolio and to provide the best possible geographical coverage to life scientists across France, France-BioImaging invites node candidates to submit their letter of intent here before May 31st, 2022.

Node candidates should:

  • Be made up of imaging facilities and R&D teams specialized in biological imaging, not necessarily grouped on the same campus but with a territorial logic.
  • Demonstrate readiness to provide open user access. The facilities must be open to the outside world beyond the regional level, i.e. to the national and international levels. Each Facility must have a valid IBiSA label, or must be in the process to have their integration in an already existing IBiSA facility validated in 2023, or must obtain this label in 2023. Each facility should have a structured organization and cost accounting. The facilities must have a reservation website for access to the equipment and a user training program. The facilities will be required to have or commit to a data management plan.
  • Gather R&D teams with a high degree of innovation and demonstrate a willingness to collaborate with the facilities. They must explain and demonstrate their technology and/or know-how transfer methods, particularly towards the local platforms, but not exclusively.
  • Bring real added value in relation to the current status of France-BioImaging, whether in terms of technologies, methodologies, scientific themes, novel user communities,… (for more information, visit the FBI website and consult the service offer). This is a key criteria.
  • Have an integrated and effective governance model.

More information on the selection criteria, expected commitments and documentation that needs to be provided can be found here.

Node selection will be done via a two-step process:

  • a letter of intent (deadline May 31st, 2022): the form to be completed is available here
  • a full application (deadline September 30th, 2022).

Applications will be reviewed at both steps by an International Board and decisions will be communicated at the end of 2022.

If at any time of the application process you have questions, please feel free to contact the France-BioImaging coordination Team by sending an email to contact@france-bioimaging.org.

We are looking forward to receiving your letter of intent!