FBI-AT 2024 – Description

Light sheet fluorescence microscopy is a rapidly expanding imaging technique that has been elected method of the year in 2014 by the Journal “Nature Methods”. Thanks to its abilities unique among fluorescence microscopy methods, LSFM is revolutionizing a large number of areas of research: biology of development, neuroscience, plant imaging, cell biology, oncology, virology, immunology….

This technology makes it possible to image three-dimensional samples on very vast scales ranging from the entire animal to the individual cell and the single molecule, and this, in a very minimally invasive way. Its very high imaging speed combined with excellent spatial resolution makes possible the rapid acquisition of large volumes with a high level of detail.

This advanced training aims to (1) present the theoretical bases, (2) clarify and synthesize the different existing approaches to both sample and instrumental preparation and (3) provide an overview of treatment and management solutions of acquired data. These objectives will be addressed through two biological fields of application: Neurosciences and 3D Cell Cultures.

The course will have plenary lectures given by experts in the microscopy development field. These seminars will be advertised as a series and will be broadcasted for a large audience. In addition, specific techniques will be introduced.

Hands-on practicals will train attendants on these techniques every afternoon in Bordeaux at the Bordeaux Imaging Center (BIC). Access to this part of training will be restricted to selected and registered trainees.

To guaranty access to set-ups and proper training, each practical session will host only 3-4 persons. The sessions will be run in parallel.

Registration will be announced soon! 

FBI-AT is ideal for researchers with a basic training in microscopy willing to become familiar with advanced techniques to answer their specific biological questions, or to be exposed to new developments that will allow them to tackle new questions in their project. We will consider applications from early career researchers (PhD students, post-docs), technical staff members and more senior scientists.