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Job offer

@ UMR703 PAnTher INRAE/ONIRIS

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  • Super Resolution Microscopy
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Microscopy Engineer/Microscopist (M/F)

Organization: UMR703 PAnTher INRAE/ONIRIS

Location: 101 Route de Gachet, 44300 Nantes, France
Send CV, publications, summary and references to:
Laurence Dubreil at Laurence.dubreil@oniris-nantes.fr

Position Information:

Description of the mission
The engineer will support APEX by maintaining different state of the art wide-field, confocal,multispectral, multiphoton and super-resolution (STORM/PALM/AiryScan/TIRF) microscopes; will contribute to user training at microscopes and image analysis; and will participate in the R&D activities in issue phenotyping and cell tracking.. Further information regarding the platform including the detailed list of equipment can be found at https://www6.inrae.fr/anatomie_pathologique_sante_animale.

Working Environnement and scientific context
APEX (Anatomical Pathology Expertise for research) is a platform dedicated to veterinary pathology and fluorescence bio-imaging and focused on animal model characterization. Located at the Veterinary School of Nantes (Oniris) and set up in 2006 by the joint research unit 703 PAnTher, member of NeurATRIS, APEX carried out by the National Institute of Agronomic Research (INRA) and by Oniris is labelled IBiSA and BioGenouest, and certified ISO 9001.
APEX is focused on the investigation of healthy and diseased animal tissues. To address scientific challenges, APEX is structured around experts with complementary skills offers integrated veterinary histopathology and
fluorescence bio-imaging to scientific community, private or academic. Thanks to an original synergy between the knowledge of normal and pathologic animal tissue and skills in bio-imaging, APEX initiates new
developments with a constant concern to strengthen the technological and methodological offer in the field of tissue and cell phenotype (level of detection, tracking, quantification, specific multi-labelling, quantitative
histopathology).
APEX originally proposes an integrated offer in animal tissue phenotype and synergizes the skills of veterinary pathologists, certified by the European board committee and of engineers R&D specialized in fluorescence bioimaging. APEX facility core can set-up pathology and bio-imaging services in:
– all types of tissues and organs with an additional long-term recognized experience in neuropathology and muscle pathology;
– a very large range of species (from animal models, with a solid track record in large ones, to livestock target species and wild sentinel species) as veterinary pathologists are specially trained to comparative pathology;
– studies compliant with drug regulatory agencies (EMA, FDA…);
– studies requiring leading-edge bio-imaging technology (biphotonic, super-resolution microscopy PALM/STORM, spectral imaging, slide scanner…).

Major tasks
 Choose and customize imaging systems to deal with scientifics purposes
 Develop new methodologies in bio-imaging and sample preparation
 Analyse and interprete microscopic images and clearly present results.
 Train and ensure technology transfer to platform users
 Advise and train users for sample preparation and analytical methods

 Develop solutions for image analyses
 Write technical notes and tutorials
 Scientific and technical monitoring in fluorescence bio-imaging
 Design and implement trainings

Skills
 Knowledge and experience in confocal microscopy, high resolution, multiphoton.
 Skills in optic will be appreciated
 Know how to use informatic tools and softwares dedicated to image acquisition and image analyses (FIJI, NIS, Huygens, Amira)
 Skills in informatic will be appreciated
 Plan activities of the platform in accordance with external constraints and deadlines
 Communicate and manage relationships with collaborators

Personal capacities
 Enthusiastic person with a technical or research background
 University degree in life sciences or physics (master, Engineer or PhD)
 Practical experience in at least two or more of the following fields: Optics, light microscopy techniques (widefield and/or confocal), image processing.
 Rigorous, reliable, well organized
 Autonomy in the management of the requested missions
 Intitiatives and organization skills
 Education abilities, sense of contact, courtesy

Required degree
 University degree in life sciences or physics (master, Engineer or PhD)

Langage
French language skills
Skills in English greatly appreciated

Workplace
Nantes-Atlantic National College of Veterinary Medicine, Food Science and engineering (Oniris)
Contacts : Laurence.dubreil@oniris-nantes.fr (Resp. Bio-imaging core facility)
Marie-anne.colle@oniris-nantes.fr (Scientific and administrative manager of APEX)

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