Member of C2RT (Center for Resources and Research in Technology) the Unit for service and technology in ultra-structural bio-imaging  (UBI) at Pasteur provides technical and scientific support in electron microscopy, mainly, but not exclusively, to the Pasteur community, analyzing host-pathogen interactions at the ultra-structural level. Our equipment enables us to do state-of-the-art EM for life sciences that includes three dimensional EM by tomography and focused ion beam SEM. The ultrapole is run by ten members (eight engineers and two technicians), each specialized in certain techniques. We put a big emphasis on correlate light- and electron microscopy to study key (rare events) of host-pathogen interactions and we have designed several protocols to combine room temperature LM and cryo-LM with FIB-SEM, cryo-SEM and cryo-tomography.

The UBI also has a small research group focusing on large DNA-viruses. Through this research theme we wish to establish and develop robust, new protocols for various EM-techniques.

The BIC (Bordeaux Imaging Center) offers resources in photonic and electronic imaging, mainly in life, health and plant sciences. It is a core facility identified at the national level as IBISA that gathers 12 highly skilled engineers. It has the ISO9001 label. The different components of the BIC are: PHOTONIC imaging, ELECTRONIC imaging, PLANT imaging. The Bordeaux Imaging Center offers access to the most advanced bio-imaging techniques for fixed and live cell imaging such as video-microscopy, confocal microscopy, multiphoton microscopy, transmission electron microscopy and scanning electron microscopy. The BIC provides a unique set of high-end equipment for super- resolution microscopy such as STED confocal microscopy, FRAP video-microscopy, lifetime imaging FLIM for the measurement of molecular interactions. We also provide access to equipment for sample preparation such as ultra-microtoms, high pressure freeze (HPF) and we can host live samples.

Microscopy systems available @BIC

ImagoSeine imaging facility gathers advanced light microscopy, cytometry and electronic microscopy activities, installed on 450 m² at IJM. Rooms for cells and tissues manipulations are provided to external users. ImagoSeine has been assessed and found to meet the requirements of ISO 9001 by AFNOR Certification. ImagoSeine brings together technologies and expertise in these fields, thanks to the 7 permanent and 2 contractual engineers. The originality of ImagoSeine-IJM is the close collaboration between the imaging facility which have a long experience of making available standard approaches but also new developments to the biological community and a research team involved in the development of state of the art functional imaging technologies (FLIM-FRET; FCCS) and more recently superresolution and non-linear microscopy. The ultimate goal is to provide access and training to these emerging techniques and methods for the realization of competitive biological projects.