Multiscale imaging of complex 3D objects: from embryonic to subcellular imaging (adaptive confocal and two-photon microscopy, light-sheet imaging of cleared tissues; NIR detection of luminescent probes, IA-based image analysis)
Quantitative FRET and stoichiometry measurements to map biological activity in cells : fluorescence quantification via an universal QuanTI-FRET protocol
Quantification of density and structure of biofunctional surfaces (FRAP and reflection interference contrast microscopy-RICM) and nanometric dynamic cell-substrate distance mapping
Gene expression, DNA damage, DNA structure and DNA-binding proteins structures, super-resolution (SMLM, RIM, SIM, Airyscan), single particle tracking, photo-manipulation, Correlative Light Electron Microscopy (CLEM)
To decipher Inflammatory and cardiovascular diseases, multiplex imaging and cytometry pipelines include the entire process from sample preparation, image acquisition to image analysis and quantification. Our workflows are adpated to mouse or human lymphoid organs and blood . They employ techniques such as confocal spectral imaging and cytometry-based analysis (spectral, sorting, and analysis).
To tackle complex biological questions in aging and geroscience, we design and implement advanced multimodal, multiparametric, and multiscale pipelines in imaging, flow cytometry, and image flow cytometry. Our workflows span the entire process—from sample preparation to data acquisition and multidimensional analysis. We investigate biological systems across multiple scales, from subcellular structures such as mitochondria to organoids, whole organs, and even entire organisms. Our pipelines are tailored to a wide range of tissues, including adipose tissue, muscle, bone marrow, skin, blood, and periodontal tissue. In addition, our platforms provide access to complementary techniques such as second- and third-harmonic generation (SHG, THG), spectral confocal imaging, high-content imaging, image-based cytometry, conventional and spectral flow cytometry, and mechanobiological analysis. These integrative approaches are designed to support comparative and translational research across multiple species, including fish (with an emphasis on the African turquoise killifish as a model of aging), drosophila, mice, and humans.
Inflammatory and cardiovascular diseases are complex, multifactorial conditions that affect multiple organs and cellular components. To tackle these intricate biological challenges, we employ and advance multi-modal and multi-scale imaging and flow cytometry pipelines. These pipelines include the entire process from sample preparation, image acquisition to image analysis and quantification. Our workflows are adpated to various tissues, including the heart, kidneys, blood vessels, muscles, liver and adipose tissue. They operate across scales ranging from whole organs to subcellular levels, employing techniques such as widefield imaging, confocal spectral imaging, high-resolution imaging (Airyscan, Opera Phenix), high-content imaging (Axioscan 7), and cytometry-based analysis (spectral, sorting, and analysis).
Inflammatory and cardiovascular diseases are complex, multifactorial conditions that affect multiple organs and cellular components. To tackle these intricate biological challenges, we employ and advance multi-modal and multi-scale imaging and flow cytometry pipelines. These pipelines include the entire process from sample preparation, image acquisition to image analysis and quantification. Our workflows are adpated to various tissues, including the heart, kidneys, blood vessels, muscles, liver and adipose tissue. They operate across scales ranging from whole organs to subcellular levels, employing techniques such as widefield imaging, confocal spectral imaging, high-resolution imaging (Airyscan), high-content imaging (Axioscan 7, Opera Phenix), and cytometry-based analysis (spectral, sorting, and analysis).
Organoids (lung, endometriosis, digestive tract, adipose tissue, bone marrow, skin, bladder, fallopian tube): integrative analysis (sample preparation, acquisition – light sheet, confocal, two-photon, high-content screening, multiparametric analysis, 3D imaging, analyses, flow cytometry and imaging)
Cell-biology expertise: providing cells(epithelial, glial, neuronal) and organoïds, labelling (transfections or probe-Test), and imaging for physicist, chemist that would like to test a prototype. Including : Guidance on super-resolution modalities (collaboration) : from system choice (SIM-STED-STORM) to images processing and data analysis (tools development).
Functional microscopy at the molecular, cellular, and tissue levels (FLIM-FCS, FRET, FRAP, laser ablation)
Super-resolution acquisition coupled to high-end atomic force microscopy