Contexte/Environnement

L’UTechS PBI (Photonic BioImaging) est une unité de technologie et de services en imagerie dédiée à l’étude des maladies infectieuses, systémiques et tumorales. Nos activités incluent la prestation de services, la formation, la recherche axée sur la technologie et le développement technologique et sont hautement multidisciplinaires et collaboratives. Ainsi, nous offrons l’accès à plus d’une vingtaine de systèmes d’imagerie maintenus par sept ingénieurs dédiés aux interactions avec les utilisateurs. Ces systèmes couvrent les besoins en  microscopie optique à champ large, confocale classique et à disque rotatif, multi-photonique, à illumination structurée, à onde évanescente, à feuillet de lumière, l’analyse haut contenu et la super-résolution.

Le service sur la plateforme offre aux chercheurs :

– Des technologies et expertises de pointe dont les performances et la pérennité sont assurées par du personnel compétent dédié;

– Une contribution originale en termes de développement technologique pour les projets de recherche qui l’exigent;

– Un accès au matériel sous plusieurs formes, allant de l’accès autonome après une formation spécifique validée par un expert de la plateforme à la séance assistée (service ou accompagnement complet), en passant par la mise en place de projets collaboratifs incluant du développement technologique si nécessaire.

Afin de renforcer son offre de service, l’UTechS PBI recherche un(e) ingénieur H/F junior pour rejoindre son équipe. Au sein de la plateforme d’imagerie et en coordination avec le responsable, l’ingénieur  participera au bon fonctionnement du service, avec les missions suivantes :

Missions/Activités 

– Analyse des besoins des utilisateurs et avoir la capacité à les traduire en spécifications techniques

– Participation aux formations et assistances des utilisateurs en français et en anglais, particulièrement pour les systèmes à champs large et confocaux (scanning et disque rotatif).

– Pilotage de ces mêmes systèmes optiques avec les utilisateurs non autonomes.

– Implication au quotidien dans le fonctionnement de la plate-forme au niveau des systèmes d’imagerie (maintenance, gestion des incidents et interventions en lien avec les constructeurs).

– Veille technologique par participation et/ou organisation d’évènements scientifiques : séminaire, workshops.

– Participation optionnelle possible aux travaux de développement sur des techniques avancées d’imagerie.

Compétences attendues

Compétences techniques :

– Maitrise de microscopie optique sur échantillons fixés ou vivants en champs large et confocal; connaissances en techniques de pointe (super résolution, multiphotonique, imagerie haut contenu…..) seraient un plus.

– Notions de traitement et analyse d’images (connaitre les prérequis essentiels à la préparation d’échantillon en imagerie photonique)

Compétences biologiques :

– Connaissances des risques associés à la manipulation d’échantillons biologiques.

– Expérience pratique souhaitée en culture cellulaire et préparation d’échantillons pour la microscopie.

– une expérience en plateforme d’imagerie, même minime est fortement souhaitée

Qualités personnelles :

Du fait de l’emploi en plateforme de service, les qualités personnelles suivantes sont essentielles :

Autonomie et adaptabilité aux nouvelles technologies

Capacité à communiquer avec les utilisateurs et sens relationnel

Esprit d’équipe

Disponibilité

Réactivité et sens de l’initiative

Niveau d’anglais opérationnel

Contrat à durée indéterminée

Several postdoctoral positions are opened at the Institut Pasteur (Paris, France) to visualize the topological and functional dynamics of small regulatory pieces of DNA, called enhancers, in the animal genome. Successful candidates will join a collaborative and interdisciplinary venture in the newly formed unit Physics of Biological Function of biophysicist Thomas Gregor.

Presentation of the unit and its research topics:

The Unit for the Physics of Biological Function at Institut Pasteur studies the basic physical principles that govern the existence of multicellular life. A core focus of the lab is to understand biological development–the complex process through which an organism grows from a single cell into a differentiated, multicellular organism–from a physics perspective. As such, we formulate and experimentally validate quantitative models that describe how individual cells interact and organize in order to generate complex life forms. Our main interests lie in:

  • multicellular pattern formation
  • transcriptional regulation in the context of development
  • molecular limits to biochemical sensing
  • emergence of collective behaviors in multicellular system
Description of a representative project:

The dynamic organization of the genome in time and space plays a crucial role in the functional specification of a cell. In particular the interplay between multiple distant enhancers and their target gene promoters has critical mechanistic consequences on gene activity patterns during cell differentiation and development. We are developing state-of-the- art high-resolution live imaging techniques to resolve multiple enhancers in space and time to correlate the 3D motion of the DNA polymer with gene activity. The challenge is to develop the right imaging modalities that optimize our need for high temporal and spatial resolution, and to image a large field of view with multiple (≥ 4) colors simultaneously. [For more information see: Chen et al. (2016). Direct visualization of transcriptional activation by physical enhancer-promoter proximity. bioRxiv 099523; doi: https://doi.org/10.1101/099523.]

Expected profile of the candidate:

Candidates will have a strong interest for collaborative and interdisciplinary research. They should have a proven successful track record equipped with a combination of the following skills:

  • live-cell microscopy, single molecule imaging
  • microscope design and implementation
  • hard- and software design for microscope control
  • computational image analysis

Ability to work independently and in collaboration with members of the lab and international collaborators in a dynamic, diverse and multinational group is essential. English is the working language.

Contact: thomas.gregor@pasteur.fr Applications should include a statement of research interests and motivation, a CV, and contact information for three references. Applications will be reviewed as soon as they are received. Funding is available for multiple positions but candidates will be encouraged to apply for independent competitive grants. Long-term funding is possible upon mutual agreement. Alternative projects that match with the overall goals of the unit can be discussed at the interview stage.

The newly founded unit Physics of Biological Function of biophysicist Thomas Gregor at Institut Pasteur is seeking to fill two open position at the engineer level to perform microscopy and imaging related research. Candidates with strong interest in interdisciplinary optical microscopy and biophysics research will be expected to build microscopes, run live imaging experiments and execute image processing and analysis routines.

Presentation of the unit and its research topics:

The Unit for the Physics of Biological Function at Institut Pasteur studies the basic physical principles that govern the existence of multicellular life. A core focus of the lab is to understand biological development–the complex process through which an organism grows from a single cell into a differentiated, multicellular organism–from a physics perspective. As such, we formulate and experimentally validate quantitative models that describe how individual cells interact and organize in order to generate complex life forms. Our main interests lie in:

  • multicellular pattern formation
  • transcriptional regulation in the context of development
  • molecular limits to biochemical sensing
  • emergence of collective behaviors in multicellular system
Description of a representative project:

The dynamic organization of the genome in time and space plays a crucial role in the functional specification of a cell. In particular the interplay between multiple distant enhancers and their target gene promoters has critical mechanistic consequences on gene activity patterns during cell differentiation and development. We are developing state-of-the- art high-resolution live imaging techniques to resolve multiple enhancers in space and time to correlate the 3D motion of the DNA polymer with gene activity. The challenge is to develop the right imaging modalities that optimize our need for high temporal and spatial resolution, and to image a large field of view with multiple (≥ 4) colors simultaneously.

Expected profile of the candidate:

Candidates will have a strong interest for collaborative and interdisciplinary research. They should have a proven successful track record equipped with a combination of the following skills:

  • live-cell microscopy, single molecule imaging
  • microscope design and implementation
  • hard- and software design for microscope control
  • computational image analysis

Ability to work independently and in collaboration with members of the lab and international collaborators in a dynamic, diverse and multinational group is essential. English is the working language.

Contact: thomas.gregor@pasteur.fr Applications should include a statement of research interests and motivation, a CV, and contact information for three references. Applications will be reviewed as soon as they are received. Funding is available for multiple positions; long-term funding is possible upon mutual agreement.

The BIC (Bordeaux Imaging Center) offers resources in photonic and electronic imaging, mainly in life, health and plant sciences. It is a core facility identified at the national level as IBISA that gathers 12 highly skilled engineers. It has the ISO9001 label. The different components of the BIC are: PHOTONIC imaging, ELECTRONIC imaging, PLANT imaging. The Bordeaux Imaging Center offers access to the most advanced bio-imaging techniques for fixed and live cell imaging such as video-microscopy, confocal microscopy, multiphoton microscopy, transmission electron microscopy and scanning electron microscopy. The BIC provides a unique set of high-end equipment for super- resolution microscopy such as STED confocal microscopy, FRAP video-microscopy, lifetime imaging FLIM for the measurement of molecular interactions. We also provide access to equipment for sample preparation such as ultra-microtoms, high pressure freeze (HPF) and we can host live samples.