Connecting different resolutions for correlative confocal/SEM and confocal/TEM on adherent cells
Gene expression, DNA damage, DNA structure and DNA-binding proteins structures, super-resolution (SMLM, RIM, SIM, Airyscan), single particle tracking, photo-manipulation, Correlative Light Electron Microscopy (CLEM)
Serial sectioning, SBF, FIB-SEM
Correlative light/electron microscopy (CLEM)
Workflow from sample preparation, MET and cryo-confocal, advanced photonic microscopy
WF/confocal/FLIM, CLEM, image analysis (AMIRA)
Talos F200X G2
Stellaris 8, WLL, FALCON, Leica Microsystems
Volumetric electron microscopy : from sample preparation (chemical fixation), to imaging & data processing
(Key-words: SBF, Array-tomography, µCT, micro-wave, nanotomy)
With the help of the IBDM Lignt Microscopy Facility we can offer an comprehensive CLEM Pipeline from live fluorescence imaging to EM imaging (in 2D and 3D)
CryoCLEM microscopy combines cryo-electron microscopy (CryoEM) with cryo-light microscopy, enabling the correlation of molecular localization with high-resolution structural details in frozen samples.
This approach preserves the native state of biological samples, providing more accurate insights into their molecular and structural organization.
Correlative light/electron microscopies imaging for plant sciences: from sample preparation, to imaging & data processing
(Key-words: cryo-preparation for resin embedding, electron tomography, correlation, image analysis)
Cryo-nano CLEM: Super-resolution-based correlative light/electron microscopies imaging in cryogenic conditions for neurosciences. From sample preparation, to imaging & data processing
(Key-words: STORM, PALM, PAINT, DNA-PAINT, SRRF, PALM-Tracer, cryo-TEM, tomography, data analysis)