Contexte et environnement de travail

La Plateforme ICAP est spécialisée dans la visualisation et l’analyse de la structure et de processus dynamiques au niveau cellulaire et tissulaire jusqu’à l’échelle de l’organisme. Elle a pour mission de mettre à disposition des biologistes et des médecins des instruments d’optique et des outils d’analyses de pointe accompagnés d’une expertise.

Elle comprend aujourd’hui 2 personnes : 1 IGR et 1 ASI. Pour la description des équipements, voir : https://icap.u-picardie.fr

Missions

Au sein de la plateforme d’imagerie et en coordination avec la responsable, l’ingénieur – e en expérimentation et instrumentation biologiques participera au bon fonctionnement du service, avec les missions suivantes :

–  Conseiller, assister, former, les utilisateurs sur les différents systèmes d’acquisition

–  Choisir, adapter et développer de nouvelles stratégies d’acquisition en réponse aux besoins des utilisateurs,

–  Participer au fonctionnement général de la PF,

–  Participer à la gestion du parc d’instruments et à son évolution,

–  Assurer le bon fonctionnement des appareils de microscopie,

–  Prendre en charge le pôle transparisation : développer des techniques de transparisation sur des organismes et organes provenant de différents modelés animaux.

Activités principales

  • Choisir et adapter les systèmes d’acquisition d’images aux problématiques des expérimentations,
  • Former les utilisateurs du service aux différents systèmes d’imagerie disponibles,
  • Rédiger des notes techniques sur les différents systèmes et approches expérimentales,
  • Assurer l’entretien, la maintenance et le bon fonctionnement des appareils,
  • Superviser et gérer l’utilisation des appareils : réservations du planning, liaison SAV…
  • Prendre en charge les projets en transparisation d’organes et acquisition d’images en microscopie à feuille de lumière,
  • Prendre en charge les approches de co-localisations et les approches FRAP, FLIP, FRET, …
  • Assurer une veille scientifique et technologique dans le domaine de l’imagerie
    Participer à des réseaux professionnels d’échanges de savoirs et de savoir-faire,
  • Diffuser et valoriser les résultats des développements technologiques
    Appliquer et faire appliquer le règlement de l’utilisation de la PF et les règles d’hygiène et sécurité́,

Compétences requises nécessaires à la tenue du poste

  • Connaissances générales en biologie,
  • Connaissances théoriques et pratiques en microscopie photonique,
  • Connaissances théoriques et pratiques en préparation d’échantillons, immunomarquage, immunofluorescence et des protocoles de transparisation,
  • Connaissance pratique des logiciels dédies à l’acquisition d’images,
  • Connaissance pratique des logiciels de visualisation et traitements d’images (Imaris, Arivis, Image J),
  • Bon niveau d’expression et de compréhension écrites et orales en anglais,
  • Capacité d’analyse et de synthèse.

Aptitudes

  • Bon sens relationnel avec de bonnes qualités pédagogiques – Capacité de travail en équipe
    Rigueur technique et sens de l’organisation
  • Motivation et enthousiasme
  • Autonomie

Expérience souhaitée

Une expérience dans le domaine de la microscopie optique et en transparisation est fortement souhaitée

Diplôme(s) souhaité(s)

BAC+5

Salaire

Sur grille de salaires de la fonction publique

Prise de fonction

Prise de fonction : A partir de Janvier 2023 (12 mois renouvelable)

Contact

Candidature (LM+CV+Recommandations) à envoyer à paulo.marcelo@u-picardie.fr

PhD POSITION, starting in October 2023 – deadline mid-November
INSTITUT PASTEUR Paris, Research & Technology international program

Great opportunity for a PhD position on the “Structural organization of the hearing system” by using cutting edge imaging technologies including cryo-electron tomography, cryoCLEM and super-resolution optical microscopy on intact tissue samples!

The deadline is mid-november, do not miss it!

This position is funded by the Pasteur-Paris University International Doctoral Program (PPU) for Research and Technology. The research project will be supervised by Nicolas Wolff (Receptor Channel Unit, Institut Pasteur) and by Anna Sartori-Rupp at the NanoImaging cryoEM facility of Institut Pasteur Paris.

For more information about the project please follow these links:
https://lnkd.in/efjkGeVZ

The candidates will have the cutting-edge optical and cryo-electron microscopy infrastructure and expertise of Institut Pasteur at his/her disposal, including a STED microscope, and a TitanKrios G3i Cryo-TEM equipped with a SelectrisX imaging filter & a Falcon4i direct electron detector, an Aquilos cryo-FIB/SEM system with cryo-lift-out and iFLM module and a Leica cryo-CLEM cryo-fluorescent microscope.

We encourage potential candidates to apply and join us on this exciting project in a unique scientific environment in the very heart of Paris!

If you are interested, please contact us directly at:
anna.sartori-rupp@pasteur.fr & nicolas.wolff@pasteur.fr

Study of long-range electrodynamic interactions between proteins

 

Context
In living matter, a complex network of molecular cascade events involving  hundreds of different molecules, which have to diffuse, meet and interact at the correct time in the correct place, is responsible for the transmission of information through cells.

All these biochemical reactions are highly spatially organized and coordinated. This project aims at extending the paradigm of the self-organization of the intracellular living matter, by exploring in biological systems the existence of long-range electrodynamic interactions (LEDIs) on protein-protein recognition processes.
LEDIs result from condensation phenomenon, characterized by the emerging of the mode of lowest frequency, expected in the TeraHertz (THz) frequency band. However, to date, LEDIs have eluded detection, mainly because performing THz spectroscopy in aqueous media is a well-known technological roadblock not yet overcome.

In this project, the LINkS consortium will develop a breakthrough lab-on-chip THz biosensor technology to investigate LEDIs between proteins, from in vitro to in vivo. Our consortium gathers interdisciplinary expertise including theoretical physics, cell biology, nanotechnology and microfluidic engineering.

Three academic partners and two SMEs, from four European countries will work in strong collaboration, across the traditional boundaries of their disciplines, to develop a disruptive lab-on-chip THz biosensor able to investigate LEDIs in the real complexity of biological systems.

LINkS project will have significant benefits for understanding the influence of electromagnetic fields on living organisms thus opening up new fields of research in medicine and biology.

Qualifications
We are seeking an enthusiastic and talented postdoc to embark with us on this ambitious and challenging project aiming at deciphering the role of long-range electrodynamic interactions (LEDIs) on protein-protein recognition processes.
The candidate must hold a PhD in biophysics or biochemistry with excellent technical skills in fluorescence imaging and spectroscopy techniques, protein characterization, and ideally previous experience with computing tools. Experience in protein purification or molecular and cellular biology as well as a good knowledge in the physico-chemistry of proteins is also preferred.
The candidate should have a proven record of accomplishment of successful independent working. The position further requires organizational qualifications and the ability to communicate effectively with internal and external collaborators.

Missions
The candidate will be expected to play a lead role in:
(1) The selection and design of protein models;
(2) The determination of the experimental conditions providing the at best the physiological environment for stabilized protein structures tacking into account technological constraints;
(3) The engineering of the relevant biological models for liquid-liquid phase separation studies and light controlled molecular interactions;
(4) The development of analytical tools.

Offer
Within the frame of the LINkS project funded by the European Union H2020 FET Open call, this position is available for 3-years, with a flexible starting date between September and December 2021. You will work with enthusiastic consortium group leaders and join an already interdisciplinary group working at the crossroads between advanced optical imaging, biochemistry and cell biology. On a daily basis, you will collaborate with researchers, engineers and interact with the group of theoreticians and THz spectroscopy experimentalists. Salary is based on qualifications and professional experience in accordance with the CNRS policy.

Environment
Within the frame of an interdisciplinary collaboration with Professor Marco PETTINI (Centre de Physique Théorique, Marseille, France) and Associate Professor Jérémie TORRES (Institut d’électronique et des systèmes, Montpellier, France), the successful candidate will join the Centre d’Immunologie de Marseille Luminy, a public research institute dedicated to study immunology, located in the south of Marseille, France, at the gates of the beautiful Calanques National Park. The institute, which provides access to state-of-the art core facilities, has developed an organization and practices designed to foster the creativity and risk-taking of its researchers.

Application
We are looking forward to receiving your application documents, which include your CV, your list of publications, transcript of record, a motivation of your research interests (max. 1 page) and the contact details for appropriate referees in one PDF document. Please send it under the subject “Post-doc LEDI” by e-mail to Dr. Didier Marguet (marguet@ciml.univ-mrs.fr). He will also be happy to provide you with further information in advance.

The platform provides regulated access to imaging systems, as well as scientific and technical assistance in the design of projects and experiments. Proposed techniques are: epifluorescence, video microscopy, confocal, spinning disk, multiphoton, lightsheet, TEM, cryo-electron microscopy, high pressure freezing, tomography, immuno-electron microscopy.
Backed by research teams, the platform also has a strong R&D activity dedicated to the design of prototype microscopes. Finally, MRic provides training to microscopy techniques.

Several postdoctoral positions are opened at the Institut Pasteur (Paris, France) to visualize the topological and functional dynamics of small regulatory pieces of DNA, called enhancers, in the animal genome. Successful candidates will join a collaborative and interdisciplinary venture in the newly formed unit Physics of Biological Function of biophysicist Thomas Gregor.

Presentation of the unit and its research topics:

The Unit for the Physics of Biological Function at Institut Pasteur studies the basic physical principles that govern the existence of multicellular life. A core focus of the lab is to understand biological development–the complex process through which an organism grows from a single cell into a differentiated, multicellular organism–from a physics perspective. As such, we formulate and experimentally validate quantitative models that describe how individual cells interact and organize in order to generate complex life forms. Our main interests lie in:

  • multicellular pattern formation
  • transcriptional regulation in the context of development
  • molecular limits to biochemical sensing
  • emergence of collective behaviors in multicellular system
Description of a representative project:

The dynamic organization of the genome in time and space plays a crucial role in the functional specification of a cell. In particular the interplay between multiple distant enhancers and their target gene promoters has critical mechanistic consequences on gene activity patterns during cell differentiation and development. We are developing state-of-the- art high-resolution live imaging techniques to resolve multiple enhancers in space and time to correlate the 3D motion of the DNA polymer with gene activity. The challenge is to develop the right imaging modalities that optimize our need for high temporal and spatial resolution, and to image a large field of view with multiple (≥ 4) colors simultaneously. [For more information see: Chen et al. (2016). Direct visualization of transcriptional activation by physical enhancer-promoter proximity. bioRxiv 099523; doi: https://doi.org/10.1101/099523.]

Expected profile of the candidate:

Candidates will have a strong interest for collaborative and interdisciplinary research. They should have a proven successful track record equipped with a combination of the following skills:

  • live-cell microscopy, single molecule imaging
  • microscope design and implementation
  • hard- and software design for microscope control
  • computational image analysis

Ability to work independently and in collaboration with members of the lab and international collaborators in a dynamic, diverse and multinational group is essential. English is the working language.

Contact: thomas.gregor@pasteur.fr Applications should include a statement of research interests and motivation, a CV, and contact information for three references. Applications will be reviewed as soon as they are received. Funding is available for multiple positions but candidates will be encouraged to apply for independent competitive grants. Long-term funding is possible upon mutual agreement. Alternative projects that match with the overall goals of the unit can be discussed at the interview stage.

The newly founded unit Physics of Biological Function of biophysicist Thomas Gregor at Institut Pasteur is seeking to fill two open position at the engineer level to perform microscopy and imaging related research. Candidates with strong interest in interdisciplinary optical microscopy and biophysics research will be expected to build microscopes, run live imaging experiments and execute image processing and analysis routines.

Presentation of the unit and its research topics:

The Unit for the Physics of Biological Function at Institut Pasteur studies the basic physical principles that govern the existence of multicellular life. A core focus of the lab is to understand biological development–the complex process through which an organism grows from a single cell into a differentiated, multicellular organism–from a physics perspective. As such, we formulate and experimentally validate quantitative models that describe how individual cells interact and organize in order to generate complex life forms. Our main interests lie in:

  • multicellular pattern formation
  • transcriptional regulation in the context of development
  • molecular limits to biochemical sensing
  • emergence of collective behaviors in multicellular system
Description of a representative project:

The dynamic organization of the genome in time and space plays a crucial role in the functional specification of a cell. In particular the interplay between multiple distant enhancers and their target gene promoters has critical mechanistic consequences on gene activity patterns during cell differentiation and development. We are developing state-of-the- art high-resolution live imaging techniques to resolve multiple enhancers in space and time to correlate the 3D motion of the DNA polymer with gene activity. The challenge is to develop the right imaging modalities that optimize our need for high temporal and spatial resolution, and to image a large field of view with multiple (≥ 4) colors simultaneously.

Expected profile of the candidate:

Candidates will have a strong interest for collaborative and interdisciplinary research. They should have a proven successful track record equipped with a combination of the following skills:

  • live-cell microscopy, single molecule imaging
  • microscope design and implementation
  • hard- and software design for microscope control
  • computational image analysis

Ability to work independently and in collaboration with members of the lab and international collaborators in a dynamic, diverse and multinational group is essential. English is the working language.

Contact: thomas.gregor@pasteur.fr Applications should include a statement of research interests and motivation, a CV, and contact information for three references. Applications will be reviewed as soon as they are received. Funding is available for multiple positions; long-term funding is possible upon mutual agreement.

BioAxial is a high end technology company which offers the most user-friendly and advanced super-resolution technology to biologists and researchers who focus on live cell imaging. To complement its team, the company is currently seeking a talented, creative and experienced algorithm designer, with a taste for intellectual challenge, and with global image processing responsibilities; this job is based in Paris and offers potential for growth within the organization.

The company develops, manufactures and markets a super-resolution microscope for life sciences and medical research communities. After several years of research and development, BioAxial is launching a breakthrough solution based on Conical Diffraction which boosts the resolution of confocal microscopes.

Job description :

  • Responsible for algorithm design, and its implementation on the instrument
  • In charge of global image processing at BioAxial
  • Reports to CTO

Required skills :

  • Expert knowledge in Algorithm (Matlab), deconvolution, image processing and analysis
  • Port from Matlab to C++ and optionally CUDA – in collaboration with the software engineer
  • High degree (or PhD) in Image Processing and Analysis or Applied Mathematics
  • Minimum 3 years’ experience
  • Proven team work capacity
  • Fluent in English

 

The BIC (Bordeaux Imaging Center) offers resources in photonic and electronic imaging, mainly in life, health and plant sciences. It is a core facility identified at the national level as IBISA that gathers 12 highly skilled engineers. It has the ISO9001 label. The different components of the BIC are: PHOTONIC imaging, ELECTRONIC imaging, PLANT imaging. The Bordeaux Imaging Center offers access to the most advanced bio-imaging techniques for fixed and live cell imaging such as video-microscopy, confocal microscopy, multiphoton microscopy, transmission electron microscopy and scanning electron microscopy. The BIC provides a unique set of high-end equipment for super- resolution microscopy such as STED confocal microscopy, FRAP video-microscopy, lifetime imaging FLIM for the measurement of molecular interactions. We also provide access to equipment for sample preparation such as ultra-microtoms, high pressure freeze (HPF) and we can host live samples.

Microscopy systems available @BIC