PhD POSITION, starting in October 2023 – deadline mid-November
INSTITUT PASTEUR Paris, Research & Technology international program

Great opportunity for a PhD position on the “Structural organization of the hearing system” by using cutting edge imaging technologies including cryo-electron tomography, cryoCLEM and super-resolution optical microscopy on intact tissue samples!

The deadline is mid-november, do not miss it!

This position is funded by the Pasteur-Paris University International Doctoral Program (PPU) for Research and Technology. The research project will be supervised by Nicolas Wolff (Receptor Channel Unit, Institut Pasteur) and by Anna Sartori-Rupp at the NanoImaging cryoEM facility of Institut Pasteur Paris.

For more information about the project please follow these links:
https://lnkd.in/efjkGeVZ

The candidates will have the cutting-edge optical and cryo-electron microscopy infrastructure and expertise of Institut Pasteur at his/her disposal, including a STED microscope, and a TitanKrios G3i Cryo-TEM equipped with a SelectrisX imaging filter & a Falcon4i direct electron detector, an Aquilos cryo-FIB/SEM system with cryo-lift-out and iFLM module and a Leica cryo-CLEM cryo-fluorescent microscope.

We encourage potential candidates to apply and join us on this exciting project in a unique scientific environment in the very heart of Paris!

If you are interested, please contact us directly at:
anna.sartori-rupp@pasteur.fr & nicolas.wolff@pasteur.fr

The Synapse and Circuit Dynamics (SCD) team is composed of 12 people, with 1 CNRS researcher, 1 Institut Pasteur researcher in optics, 1 Institut Pasteur engineers (IR) in optics, 6 post-docs, 1 student and 1 technician. The optics part of the team is pursuing STED nanoscopy developments specifically tailored to the needs of neurobiology studies and is dedicated in particular to the development of an upright microscope design for two-photon excitation STED towards deep brain imaging. The Unit is also partner on a grant for
the dissemination of a design of a random access acousto-optic scanning two-photon microscope in collaboration with Dr. Angus Silver (UCL). The laboratory has also pioneered methods for fast calcium imaging (spot detection, Nakamura et al. 2015) voltage dye imaging, and photolysis of neurotransmitter (uncaging, DiGregorio et al. 2007; Tran Van Minh et al. 2020).

  • David DiGregorio is currently the director of the shared 2-photon imaging facility for the Neuroscience Department. SCD team member, Dr. Ruckerl manages the training and usage of the microscopes, while Dr. Moneron provides advice and design suggestions. There are three 2-photon microscopes (2 of which are embedded in a BSL2 animal facility).
  • The SCD also established the first fluorescence microscopy course (since 2016) at Institut Pasteur and continues to direct this course.
  • The SCD team is currently engaged in a project to install a small footprint 2-photon microscope in a BSL3 facility for imaging live infected animals, and in particular those mice infected with SARS-CoV2.

The BIC (Bordeaux Imaging Center) offers resources in photonic and electronic imaging, mainly in life, health and plant sciences. It is a core facility identified at the national level as IBISA that gathers 12 highly skilled engineers. It has the ISO9001 label. The different components of the BIC are: PHOTONIC imaging, ELECTRONIC imaging, PLANT imaging. The Bordeaux Imaging Center offers access to the most advanced bio-imaging techniques for fixed and live cell imaging such as video-microscopy, confocal microscopy, multiphoton microscopy, transmission electron microscopy and scanning electron microscopy. The BIC provides a unique set of high-end equipment for super- resolution microscopy such as STED confocal microscopy, FRAP video-microscopy, lifetime imaging FLIM for the measurement of molecular interactions. We also provide access to equipment for sample preparation such as ultra-microtoms, high pressure freeze (HPF) and we can host live samples.

Microscopy systems available @BIC