Following the final decision of France-BioImaging Institutional Committee on February 21^th, 2023, we are delighted to announce that two new nodes are joining France-BioImaging: the Alsace Node and the Toulouse Node.

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The new Alsace Node is composed of six imaging facilities: QuEST, the Cell Imaging Facility from IBMP, PIV, PI2, PIC-STRA and PMC. The node has also six highly visible R&D teams (from IRIMAS, LBP, ICube, IGBMC and IPHC) expert in microscopy techniques and tools.

Located in Strasbourg, Illkirch and Mulhouse, the Alsace node is offering high level technical and innovative methodological expertise in multi-scale imaging at the interface between biology, chemistry, optics and physics, from the atom to the small animal/plant.

The Alsace Node has a strong expertise in probes with the development of highly innovative fluorescent probes and luminescent nanoparticles.

The node provides cutting-edge technologies and methodologies, with among others:

• Tomographic diffractive microscopy, for 3D label-free imaging at cellular level, with an improved resolution compared to conventional microscopes, and, not being limited by possibly weak fluorescence, potentially at high speed (several 3D images/s)
• Single particle tracking and time-resolved luminescence microscopy, to image upconverting nanoparticles (UCNPs). Due to their anti-Stokes emission, UCNPs allow imaging applications with exceptional signal to noise ratio.
• Single-shot full-field optical coherence tomography, extracts an FF-OCT image from a single interference acquisition, enabling single-shot high-resolution imaging within turbid media such as biomedical samples.
• SharpViSu & ClusterViSu, development of an integrated software for image reconstruction, correction, co-localization, resolution estimation, segmentation and clustering of labelled complexes
• A large range of molecular and nanoparticle probes for SR and advanced microscopy techniques as well as molecular and supramolecular complexes for anti-Stokes imaging at the molecular level have been developed.

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The new Toulouse node is composed of a large nationally recognized multi-site core facility: Toulouse Reseau Imagerie. Distributed on Toulouse greater area, the facility is divided between medical science, fondamental science, cancer and rejuvenation, and agro-bio science. Moreover, eight R&D teams complete the node and supports the facility (from LAAS, CBI, IPBS, IRSD and IMT).

The Toulouse node aims to maintain the level of scientific excellence in response to the needs of users and the concerns of host laboratories. Four scientific axes are conducted: mechano-biology, molecules and single cells, whole organisms, image processing and quantitative data analysis.

The mission of the node is also to develop original devices to explore biophysical properties in living samples, to work at the interface between machine and sample and to develop artificial intelligence applied to bioimaging.

The node provides cutting-edge technologies and methodologies, with among others:

• Random illumination microscopy, a super-resolution microscopy technique, interesting for its robustness
• Protrusion force microscopy, combination of micro-fabrication, imaging approaches, including Atomic Force Microscopy, dSTORM coupled to supercritical angle fluorescence, and random illumination
• Cryomethods for electron microscopy, includes sample preparation of frozen samples for cryo-microscopy, in view of bridging structural biology and cell biology.
• ANchOR technology, a labeling system based on ParR/ParB labeling system from bacteria, which allowed the strong fluorescent labeling of genomic site with small DNA insert.
• Microfabrication for organoids, 3D bioprinted scaffolds have been adapted to permit 3D characterization at the organoid and tissue scale

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Welcome to the FBI Alsace Node and the FBI Toulouse Node!