On December 13th and 14th 2022, we will have the pleasure to invite you to our Annual Meeting, to be hosted by FBI Bretagne-Loire Node at the Health Research Institute of the University of Nantes (Nantes, France).
2022 is an important landmark for France-BioImaging and its community, as the infrastructure is celebrating 10 years of operation and scientific advances. We will be happy to celebrate this milestone with all the members of the bioimaging community (within and outside the France-BioImaging community).
The Annual meeting will highlight France-BioImaging’s development as a research infrastructure and its node community accomplishments during these last 10 years, and the role they play in boosting innovation in bioimaging. Imaging scientists and users from the infrastructure’s nodes will present their key projects and demonstrate how they have profited from France-BioImaging and its community.
Advancing multimodal microscopy of thick samples for preclinical studies
The new France-BioImaging preclinical microscopy Working Group, whose aim is to advance multimodal microscopy of thick samples for preclinical studies, is organizing its first meeting! The event will take place on Monday December 12th 2022 in Nantes. The event will present three aspects of preclinical microscopy:
Imaging technologies (correlative microscopy, thick tissue or organoid microscopy, light sheet microscopy, intravital imaging and label free imaging)
Image analysis (whole slide, correlative and 3D analysis)
Regulatory frameworks (animal testing ethics and best practices in preclinical development)
Call for speakers
The Working Group would like to call on speakers interested in presenting one of the several aspects that will be highlighted during the meeting and contributing to the Working Group (open to everyone). If you are interested, please get in touch:
The full program is coming soon! In the meantime, you can register here.
The meeting will cover three aspects of preclinical microscopy with presentations of 30mn and a final round table:
Thick tissue imaging
3D whole organ macroscopy
Intravital imaging (imaging chambers, exposed organs in living animal..)
Label free imaging
Whole slide imaging analysis
Correlative image analysis
3D reconstruction (distortion, alignment problems…)
Animal testing ethics (3R, Ethics Committee…)
Preclinical developments (GMP, 21 CFR Part 11 Compliance…)
The PreClinical topic is usually connected to animal studies, but one needs to take into account the following aspects :
Preclinical development, also termed preclinical studies or nonclinical studies, is a stage of research that begins before clinical trials (testing in humans) and during which important feasibility, iterative testing and drug safety data are collected, typically in laboratory animals.
Preclinical imaging usually refers to whole animal imaging techniques allowing longitudinal studies such as high-frequency ultrasound, magnetic resonance imaging (MRI) and computed tomography (CT) which are usually used for anatomical imaging, while optical imaging (fluorescence and bioluminescence), positron emission tomography (PET), and single photon emission computed tomography (SPECT) which are usually used for molecular visualizations. This macro imaging scale is proposed within the infrastructure France Life Imaging.
Multimodal imaging including the microscopy scale is largely developing. Technologies developed within FBI could benefit to the preclinical community
The communities of cell, tissue and intravital microscopies need to know each other better.
The new France-BioImaging Working Group wants to focus on the microscopic aspect of the “preclinical imaging”. It aims to develop microscopy technologies for preclinical studies used to explore highly resolved information at the organ level. It includes, for example, whole slide quantitative tissue histology, correlative microscopy, 3D from sections as well as Light sheet fluorescence microscopy for 3D whole organ imaging, intravital organ imaging or multi photon microscopy. The goal is to bridge the gaps between communities dealing with either fixed sections or living organs.
Save the date! The Electron Microscopy facility of Imagerie-Gif (I2BC, France-BioImaging), the Cryo-Electron Microscopy facility (I2BC, FRISBI) and the Cimex facility of Ecole Polytechnique are organizing a 5-day workshop from October 3rd to October 7th, 2022 on Transmission Electron Microscopy to explore the architecture of a virus in all its forms.
The aim of this workshop is to propagate knowledge about transmission electron microscopy applications and to outline the advantages of transversal studies combining structural biology and cell biology. Indeed, structural biology and cell biology approaches both use TEM but are rarely merged in the same studies.
The workshop will focus on the advantages of combining both approaches, which can be easily performed with the same equipment. The workshop will focus on a biological object whose study requires such multiscale approaches: a virus. The virus will be studied in vitro to resolve its high-resolution 3D structure, and will be observed in infected cells to determine the infection and replication mechanisms in situ.
The workshop targets students and young researchers. The training will focus on a given biological object, a virus, which will be studied by two complementary approaches:
Single particle analysis by cryo-electron microscopy, allowing high-resolution 3D reconstruction of particles purified in vitro. This part will be performed on a 200kV TEM on the Cimex facility.
Cellular tomography of infected cells with observation of the virus replication sites in situ and analysis of its interaction with cellular membranes. This workshop will cover the workflow from sample preparation and resin sections realisation, to acquisition and analysis of tomograms with a 120kV TEM.
Attendees will have a theoretical and practical overview of these two complementary techniques. The practical training will be particularly emphasised, to ensure that attendees will be able to apply the knowledge acquired in the workshop for their further research projects.
The first edition of “Digital Spaces for Research and Medicine” will take place at Institut Curie onJuly 7th, 2022. This event will be dedicated to providing scientists and clinicians insight into Virtual Reality, Augmented Reality and Mixed Reality based tools that offer novel and powerful ways for visualizing and analysing data in research and medicine. This day will include presentations of the tools followed by hands-on demonstrations. The day will close with an open forum for questions and discussing future perspective. The following tools will be presented:
MorphoNet-VR (Serpico-STED, Inria-UMR144 Institut Curie and LIRMM): Visualizing live microscopy data (3D+T) in VR
Genuage (Team LOCCO, UMR168, Institut Curie): visualization and analysis of multidimensional point cloud data, such as single molecule data, in virtual reality
DIVA (Institut Pasteur, Institut Curie): 3D reconstructions of raw experimental image stacks that are integrated in virtual reality for volumetric analysis
Tamed Cloud (ENSADLab): immersive VR experience to interact with cloud of images
HoloTracks (TEAM INRAE-Unité MaIAGE / AVIZ Inria, Paris Saclay): Immersive (AR) visualization of dynamic compounds in living cells
UnityMol A&VR (UPR 9080, IBPC) : deep inside molecules – digital twins at the nanoscale
GRenoble OliGomérisation GROG will cover the latest innovations
in imaging techniques and analysis methods to quantify oligomerization and
clusterization in live cells.
The meeting will mostly deal about Single molecule localization microscopy and Fluorescence fluctuation methods and will be concluded with a round table.
Les inscriptions pour la seconde édition du TED ImaBio lundi 2 mai à Montpellier sont ouvertes.
Le GDR ImaBio vous propose de venir decouvrir les dernières innovations des partenaires industriels du GDR , et pour les etudiants un focus sur les métiers du domaine.
The 7th edition of OPTOGEN will bring developers and end-users in the field of Optogenetics and Neurophotonics together. It will be a great occasion to meet in person for stimulating discussions and to reignite collaborations.
Registration and abstract submission are open.
Early registration will close on April 15th.
Thanks to the support of the IHU Foresight and of the sponsors, inscription is free for students registered before April 15th and some travel grants will be available.
Euro-BioImaging is organizing a third online User Forum on Tuesday, April 5, 2022 from 14:00-17:00 CEST. The topic is “At the Forefront of Neuroscience.”
This event will highlight the importance of cutting-edge imaging technologies in support of brain research and showcase the specific expertise available at our Nodes across Europe through case studies presented in tandem with the research community.
In addition, keynote presentations from Hervé Boutin, University of Manchester, and Selma Ding, Max Planck Institute for Animal Behavior, will further reveal the potential of biological and biomedical imaging technologies to boost brain research.
The deadline for this inter-infrastructure access call is 15 April 2022.
All submitted proposals will be peer-reviewed by independent experts and the final funding will be approved by a committee comprising 2 representatives from each infrastructure as well as representatives from GIS IBISA. We advise the project PI to contact the chosen facilities in order to set-up the optimal experimental design.
Understanding how development is coordinated in multiple tissues and gives rise to fully functional organs or whole organisms necessitates microscopy tools. Over the last decade numerous advances have been made in live-imaging, enabling high resolution imaging of whole organisms at cellular resolution. Yet, these advances mainly rely on mounting the specimen in agarose or aqueous solutions, precluding imaging of organisms whose oxygen uptake depends on ventilation.
Engineers from the Institut Curie and Institut Jacques Monod implemented a multi-view multi-scale microscopy strategy based on confocal spinning disk microscopy, called Multi-View confocal microScopy (MuViScopy).
MuViScopy enables live-imaging of multiple organs with cellular resolution using sample rotation and confocal imaging without the need of sample embedding. They illustrated the capacity of MuViScopy by live-imaging Drosophila melanogaster pupal development throughout metamorphosis, highlighting how internal organs are formed and multiple organ development is coordinated. They foresee that MuViScopy will open the path to better understand developmental processes at the whole organism scale in living systems that require gas exchange by ventilation.
Olivier Leroy, Eric van Leen, Philippe Girard, Aurélien Villedieu, Christian Hubert, Floris Bosveld, Yohanns Bellaïche, Olivier Renaud; Multi-view confocal microscopy enables multiple organ and whole organism live-imaging. Development 15 February 2022; 149 (4): dev199760. doi: https://doi-org.insb.bib.cnrs.fr/10.1242/dev.199760
The MuViScope was co-funded by France-BioImaging.
The IGDR (Institut de Génétique et de Développement de Rennes) and MRic facilities in Rennes organize an EMBO practical course “Sensing biophysical properties in living samples using light microscopy” from July 4th to July 9th 2022.