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Winning images – FBI Image Contest 2021

Winning images – FBI Image Contest 2021

AnnouncementFBI Special Events

France BioImaging and all the French community aims to develop and promote innovative imaging technologies and methods. But microscopy images can also take an artistic, creative look and make the invisible world beautiful, allowing people to see the visual appeal of the life sciences. 

We enjoyed the diversity of the images submitted with many different microscopy techniques, models and applications represented. A big thank you to all the participants!

The National Coordination Team and the Executive Board are proud to announce the winners of the FBI Image Contest 2021:

  • 1st Place: Léna Meneux, Eye Team, Institut des Neurosciences de Montpellier

“The eye of the storm”

Sensory fibers of a mouse cornea imaged with a confocal microscope. The corneal nerves converge toward the centre forming a vortex. This particular transgenic mouse model allows stochastic expression of fluorescent proteins, unravelling the heterogeneity of the fiber origines inside the corneal epithelium.
Acknowledgements to Karine Loulier for the mouse model and Laetitia Hudececk for her help during the acquisition.

Confocal microscopy

  • 2nd Place: Eunice HoYee Chan, Muscle Dynamics Team, Developmental Biology Institute of Marseille (IBDM)
Myofibrils isolated from Drosophila indirect flight muscle labelled with titin (yellow) and actin (blue). Image captured from confocal microscope. We are studying the role of titin protein in muscle mechanics and organisation during development

“Sarcomeric bouquet”

Myofibrils isolated from Drosophila indirect flight muscle labelled with titin (yellow) and actin (blue). Image captured from confocal microscope. We are studying the role of titin protein in muscle mechanics and organisation during development.

Confocal LSM880
  • 3rd Place: Camille Boutin, Biology of multiciliated cells Team, Developmental Biology Institute of Marseille (IBDM) & Nicolas Brouilly, PICsL Imaging facility, Electron Microscopy department
Lamellar structure in a differentiating multiciliated cell observed by transmission electron microscopy with a Tecnai G2 200kV FEI.

“Clown”

Lamellar structure in a differentiating multiciliated cell observed by transmission electron microscopy with a Tecnai G2 200kV FEI.

Transmission Electron Microscopy, Tecnai G2 200kV FEI

Congratulations to the winners!

Explore all the images submitted here:

Fluorescence microscopy image illustrating skeletal muscle fibers (Desmin, green) in co-culture with motor neurons (SMI-32) forming active Neuromuscular Junction in a cell culture method using murin primary cells in vitro. Nuclei are stained by DAPI (blue).
Embryonic murine lacrimal gland (E17), with fluorescent staining showing Actin, Fibronectin, phospho-MLC and nuclei. The acquisition was performed using a confocal microscope Zeiss LSM 980, with a 25X air objective.
Confocal microscopie picture of a Drosophila larva's gut. Cyan: nuclei. Magenta: reporter for the activity of the protein GCN2. Yellow: the symbiotic bacterium Lactiplantibacillus plantarum. L. plantarum is a symbiont of Drosophila. We showed that it can communicates with its host by stimulating the activity of GCN2 in enterocytes. GCN2 may then trigger a physiological change of the gut that promotes larval growth despite malnutrition.
Representation of PALM technology on a rat hippocampal neuron. The low resolution image corresponds to the synaptic marker, Homer-GFP. Using PALM superresolution technology, it is possible to visualise NMDAR trafficking and clusters in high resolution. This approach can be used in the study of autoimmune neuropsychiatric diseases that disrupt both the organisation of NMDAR and their diffusion at the neuronal surface.
Neuronal spheroids of rat primary neurons are grown in hydrogel templates. The cells are electroporated with GFP and tdtomato. Then the spheroids are fixed and imaged with a confocal microscope. Cells in 3D structure mimic better functions and morphology properties of in vivo.
Myofibrils isolated from Drosophila indirect flight muscle labelled with titin (yellow) and actin (blue). Image captured from confocal microscope. We are studying the role of titin protein in muscle mechanics and organisation during development
The image shows the movements of Dopamine Receptors at the surface of hippocampal neurons and was acquired on a spinning disk. We took 1000 frames at 50ms exposition before reconstructing the trajectories of single receptors with PalmTracer. This allow us to understand, down to the nanometric scale, how the interaction between receptors modulate their surface diffusion.
Phages from vibro bacteria - Transmission electron microscopy
Patchwork of 4 maximum projections with different LUTs of a z-stack acquired in 2-photon microscopy. Neurons express the GCaMP6f calcium sensor following a viral infection of an organotypic mouse brain slice.
Negative staining artifact. Transmission electron microscopy.
In plants, the male gametes are represented by the microscopic round structures called the pollen. The anthers are the flower organs that produce and encase the developing pollen. This confocal microscope image is the cross-section of an anther of Arabidopsis thaliana. The developing pollen can be seen inside the anther. These are the haploid round cells containing protective pollen wall on their surface. The pollen are surrounded by diploid cell layers of the anther which nourish the young pollen supporting their development. This image was taken with the aims to understand how the growing pollen communicate with the surrounding nourishing tissues.
Aedes albopictus (Mosquito) leg imaged with a scanning electron microscope and digitally colored.
Deuterosomes producing centrioles during multiciliated cells differentiation.
Lagerstroemia sp. (Crepe Myrtle) blossom imaged with a stereomicroscope and digitally colored.
TEM observation of extracellular-vesicles from bacteria
PD1 membrane receptors super-resolution imaging in a PD1 stably expressing Jurkat cell. This acquisition has been performed with our soSPIM (single-objective Selective Plane Illumination Mocroscopy) system combined with an Adaptive Optics system (MicAO, Imagine Optique). The whole volume is composed of 35 Z-sections acquired every 500 nm, with each section consisting of 22,500 frames for a total acquisition time of 13h handled fully automatically. On each frame every molecule was precisely localized in 3D through an astigmatism-based process. The final cell exhibited more than 300,000 localizations and was 10 µm wide. To assess the spatial organization of clusters on the cell membrane, we used their centroids to compute a Voronoi diagram on the sphere approximating the cell shape.
Rat hippocampal neuron filled with a fluorescent marker and observed by dSTORM. The image shows details of dendritric spine morphology as well as surrounding axons in which we can observe the membrane-associated periodic skeleton.
Lamellar structure in a differentiating multiciliated cell observed by transmission electron microscopy with a Tecnai G2 200kV FEI.
Multiciliated cells at the surface of Xenopus embryo (Scanning Electron Microscopy)
A triple-negative cancer cell navigating its path through a dense 3D collagen matrix network. Collagen is labeled in green; Actin in magenta, and plasma membrane structure, caveolae, component in red. The image was acquired through a spinning disc confocal microscope for a 3D migration time-lapse experiment.
Ageratum bloom imaged with a scanning electron microscope and digitally colored.
Transmission electron microscopy observation of cells infected with Yersinia pseudotuberculosis in order to see their intra-vacuolar localization.
Two salivary glands from Drosophila simulans larva at L3 wandering stage. They have been stained with DAPI and rhodamine phalloidin. The upper gland is reconstructed in 3D on Imaris, nuclei and cells volumes are represented with a color scale from blue to red.

As stated in the Terms & Conditions of the contest, foreign participants non-affiliated to a French institution are featured in the gallery, but were not evaluated as part of the contest.

bioimagingImage contestMicroscopy
• December 7, 2021

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