Published on August 23rd, 2022 in EMBO reports, this article questions the way that core facilities should be recognized in the scientific literature and their key contributions to data lifecycle. An initiative endorsed by France-BioImaging.

Core facilities are an integral part of the life science research landscape as providers of centralised access to technological resources and expertise. This article’s working group has estimated that between 40 and 80% of imaging, proteomics and genomics data at their institutes are generated at core facilities. The contribution of core facilities to scientific research and innovation must thus be accordingly recognised. In that respect, the most straightforward way is an acknowledgement. Unfortunately, the lack of formal rules still leaves core facilities being inadequately recognised. 

This article proposes that the recognition of core facilities should be deployed via two actions and implemented in two phases: first, with the systematic acknowledgement of core facilities in all scientific publications, and second, by including core facilities and their staff in data citations (Cousijn et al, 2018).

The first step can be accomplished at the manuscript-submission stage by asking the corresponding author to confirm if any data (and associated metadata) used in the manuscript originated from a core facility, and if yes, to identify the associated core facility. EMBO Press has recently included a question in the author checklist to confirm whether the work in the publication “benefited from core facilities” and that the core facility be acknowledged accordingly.

The next step would be to make it compulsory for authors to respond to such a query and explicitly identify the core facility and relevant data (and associated metadata). The MDAR (Materials, Design, Analysis, Reporting) form (Macleod et al, 2021), wherein one needs to provide information about data availability in the Analysis section, could likewise include a question to explicitly identify core facilities involved. Eventually, the information in the author checklist could be automatically fed into the acknowledgement section.

Acknowledging will have two key positive consequences: on the sustainability of core facilities and on their staff careers. In the absence of a high number of publications, particularly as lead or corresponding authors, acknowledgements are used as a measure of a core facility and its staff’s output and impact. Second, it further motivates and incentivizes core facility staff to actively contribute to scientific research. 

The acknowledgement of a core facility goes beyond professional courtesy: identifying the origin of data (and associated metadata) is essential for data traceability and reproducibility particularly since core facilities are major generators of data in life science research. 

Thanks to Jean SALAMERO, our “Action inter-infrastructures” mission officer, for contributing to this article. 

Full article on:

Acknowledging and citing core facilities

Katja Kivinen, Henri G A M van Luenen, Myriam Alcalay, Christoph Bock, Joanna Dodzian, Katerina Hoskova, Danielle Hoyle, Ondrej Hradil, Sofie Kjellerup Christensen, Bernhard Korn, Theodoros Kosteas, Mònica Morales, Krzysztof Skowronek, Vasiliki Theodorou, Geert Van Minnebruggen, Jean Salamero, Lavanya Premvardhan

EMBO reports (2022) 23: e55734
https://doi.org/10.15252/embr.202255734

Developed by the Serpico Inria-CNRS-Institut Curie Joint Team, member of the IPDM-BioImage Informatics node of France-BioImaging (FBI), this open-source framework could be a huge step forward in bioimaging management and analysis.

Bioimaging has a broad range of applications, addressing a variety of biological models at diverse scales of life. Thus, descriptions of novel computational approaches are often focused on target case studies. To tackle any scenario in biological imaging is a major challenge, that needs the conception and the development of a unified solution.

With this in mind, the BioImageIT project aims at providing a middleware that integrates data management with analysis using existing softwares (Omero, BioFormats, Fiji, napari, Scipy, pytorch…). The mission of BioImageIT was to design a graphical user interface (GUI) that allows any scientist without coding skills to annotate and analyze datasets using various software. By being user-centered, open-source and cross-platform (Windows, MacOS, Linux), BioImageIT created a management tool that is definitely accessible and well documented.

Started in late 2019, the project, funded by France-BioImaging, is now being deployed in 10 FBI imaging facilities. As it is a first step, the BioImageIT project have the ambition to expand the dissemination of the middleware throughout France and even further, Europe.

BioImageIT overview. a, Schematic view of BioImageIT architecture. The BioImageIT core is composed of data management and data processing functionalities. Users can access plugins by a script editor, Jupyter or the BioImageIT graphical interface (GUI). Data management functionalities exploit local files, remote files or databases such as OMERO. Data processing can perform computations in remote jobs, containers, or local runners. Image analysis is provided by plugins written in different languages. Developers can implement their own plugins in BioImageIT and design their own Graphical Interface. (b-i) LLSM processing workflow gathered in BioImageIT. Hela cell line expressing CD-M6PR-eGFP were stained with Tubulin TrackerTM Deep Red for Microtubules. b, Due to the geometry of LLS scanning, raw 3D images are skewed. c, g, First, realignment (deskew) of raw stacks is performed using Pycudadecon. d, h, Richardson Lucy deconvolution is performed using Pycudadecon. e, CD-M6PR-eGFP vesicles are tracked using Trackmate(FiJi). f, i, Deconvolved stacks and tracks are rendered using napari.

Prigent, S., Valades-Cruz, C.A., Leconte, L. et al. BioImageIT: Open-source framework for integration of image data management with analysis. Nat Methods (2022).
https://doi.org/10.1038/s41592-022-01642-9

Going to Rendez-Vous Carnot 2022? Drop by our booth and say hello! 12 & 13 October – Paris

In a few days, we will be travelling to Paris to attend the Rendez-Vous Carnot 2022! This is the fourth time that we will attend the forum as an exhibitor, in the Research Infrastructures Village. We are going to present France-BioImaging R&D ecosystem and the multiple advanced biological imaging technology developments taking place on FBI imaging platforms and R&D teams.

If you are in Paris between October 12 and October 13 attending the Rendez-Vous Carnot as well, be sure to drop by our booth and meet some of our colleagues at the venue:

  • Caroline Thiriet , France-BioImaging External Affairs Manager
  • Etienne Henry, France-BioImaging R&D and Tech-Transfer mission Officer
  • Jean Salamero, France-BioImaging Inter-Infrastructures Activities mission Officer

This year, we are pleased to share our booth with the French Infrastructure for Integrated Structural Biology (FRISBI). This infrastucture provides integrative structural biology approaches, from the molecular to the cellular level, integrating multi-resolution data from X-ray crystallography, small angle X-ray scattering, NMR, Cryo-EM and functional data including development for protein expression and crystallization.

On December 13th and 14th 2022, we will have the pleasure to invite you to our Annual Meeting, to be hosted by FBI Bretagne-Loire Node at the Health Research Institute of the University of Nantes (Nantes, France).

2022 is an important landmark for France-BioImaging and its community, as the infrastructure is celebrating 10 years of operation and scientific advances. We will be happy to celebrate this milestone with all the members of the bioimaging community (within and outside the France-BioImaging community).

The Annual meeting will highlight France-BioImaging’s development as a research infrastructure and its node community accomplishments during these last 10 years, and the role they play in boosting innovation in bioimaging. Imaging scientists and users from the infrastructure’s nodes will present their key projects and demonstrate how they have profited from France-BioImaging and its community.

We look forward to meeting you there!

Gallery

Videos

Programme

Registration

Deadline to register: 04/12/2022 at 23:59

This form is currently closed for submissions.

Poster

INFOS PRATIQUES:

Adresse: Bâtiment IRS-UN 8 quai Moncousu 44000 Nantes

Plan d’accès https://sfrsante.univ-nantes.fr/medias/fichier/plan-acces-irs-un-1_1561112224023-pdf?ID_FICHE=1562669&INLINE=FALSE 

(arrivée directement à l’accueil, toutes les salles sont au rez de chaussé et seront fléchées)

Comment venir?

Arrêt de tram le plus proche: ligne 2 ou 3 du tramway Aimé Delrue

En train: 

Gare de Nantes à 20 minutes à Pied (préférez la sortie sud pour venir à pied). En bus ou tram compter 15 minutes.

Ligne de bus accessibles depuis arrêt sortie gare Sud C2, C3, 54 arrêt Hotel Dieu

Ligne de tram depuis la sortie Gare Nord : prendre la ligne 1 direction François Mitterand/Jamet et descendre à Commerce, continuer à pied (10 minutes de marche environ) 

En avion:

Navette aéroport  Navette aéroport Nantes Atlantique : horaires, tarif

Le départ et l’arrêt se font à cause de travaux de Hotel Dieu ( plus proche de l’IRS) et non pas Commerce. 

Taxis Nantais:

02 40 69 22 22 http://www.taxis-nantes.com/ 

02 40 85 40 85. https://heptaxis.com/ 

VTCs: 

LocalCab 09 80 66 62 82   TAXI / VTC – Réservez votre Chauffeur au Meilleur Prix ! (localcab.fr) 

Quelques Hôtels conseillés :

L’Hôtel  L’HÔTEL NANTES – Votre Boutique Hôtel idéalement placé centre-gare (nanteshotel.com) (135 à 195 euros la nuit avec petit déjeuner) 

Hôtel Amiral Hotel Nantes centre-ville – Hotel 3 étoiles – Hôtel Amiral | Nantes (hotel-nantes.fr) (128 à 150 euros avec petit déjeuner)

Hotel ibis Nantes Centre Gare Sud   ibis Nantes Centre Gare Sud – Hôtel 3 étoiles à Nantes – ALL (accor.com)  (110 à 130 euros)  ibis Styles Nantes Centre Place Royale 

Hôtel  ibis Styles Nantes Centre Place Royale Hôtel à Nantes – ibis Styles Nantes Centre Place Royale – ALL (accor.com) (85 à 95 euros) 

Prise en charge des missions: 

Se rapprocher de votre noeud FBI (fonds mission), sauf pour les intervenants qui seront directement contactés pour la prise en charge de leur missions. 

Advancing multimodal microscopy of thick samples for preclinical studies

The new France-BioImaging preclinical microscopy Working Group, whose aim is to advance multimodal microscopy of thick samples for preclinical studies, is organizing its first meeting! The event will take place on Monday December 12th 2022 in Nantes.
The event will present three aspects of preclinical microscopy:

  1. Imaging technologies (correlative microscopy, thick tissue or organoid microscopy, light sheet microscopy, intravital imaging and label free imaging)
  2. Image analysis (whole slide, correlative and 3D analysis)
  3. Regulatory frameworks (animal testing ethics and best practices in preclinical development)

Preliminary program

Registration Form

Deadline to register 04/12/2022 at 23:59

This form is currently closed for submissions.

The meeting will cover three aspects of preclinical microscopy with presentations of 30mn and a final round table:

  1. Imaging technologies
    • Correlative microscopy
    • Thick tissue imaging
    • 3D whole organ macroscopy
    • Intravital imaging (imaging chambers, exposed organs in living animal..)
    • Label free imaging
  2. Image analysis
    • Whole slide imaging analysis
    • Correlative image analysis
    • 3D reconstruction (distortion, alignment problems…)
  3. Regulatory frameworks
    • Animal testing ethics (3R, Ethics Committee…)
    • Preclinical developments (GMP, 21 CFR Part 11 Compliance…)
  4. Closing
    • Round table
    • Synthesis

Poster

France-BioImaging PreClinical Working Group

The PreClinical topic is usually connected to animal studies, but one needs to take into account the following aspects :

  1. Preclinical development, also termed preclinical studies or nonclinical studies, is a stage of research that begins before clinical trials (testing in humans) and during which important feasibility, iterative testing and drug safety data are collected, typically in laboratory animals.
  2. Preclinical imaging usually refers to whole animal imaging techniques allowing longitudinal studies such as high-frequency ultrasound, magnetic resonance imaging (MRI) and computed tomography (CT) which are usually used for anatomical imaging, while optical imaging (fluorescence and bioluminescence), positron emission tomography (PET), and single photon emission computed tomography (SPECT) which are usually used for molecular visualizations. This macro imaging scale is proposed within the infrastructure France Life Imaging.
  3. Multimodal imaging including the microscopy scale is largely developing. Technologies developed within FBI could benefit to the preclinical community
  4. The communities of cell, tissue and intravital microscopies need to know each other better.

Our Goal

The new France-BioImaging Working Group wants to focus on the microscopic aspect of the “preclinical imaging”. It aims to develop microscopy technologies for preclinical studies used to explore highly resolved information at the organ level. It includes, for example, whole slide quantitative tissue histology, correlative microscopy, 3D from sections as well as Light sheet fluorescence microscopy for 3D whole organ imaging, intravital organ imaging or multi photon microscopy. The goal is to bridge the gaps between communities dealing with either fixed sections or living organs.

Call for speakers

The Working Group would like to call on speakers interested in presenting one of the several aspects that will be highlighted during the meeting and contributing to the Working Group (open to everyone). If you are interested, please get in touch:

This form is currently closed for submissions.

Save the date! The Electron Microscopy facility of Imagerie-Gif (I2BC, France-BioImaging), the Cryo-Electron Microscopy facility (I2BC, FRISBI) and the Cimex facility of Ecole Polytechnique are organizing a 5-day workshop from October 3rd to October 7th, 2022 on Transmission Electron Microscopy to explore the architecture of a virus in all its forms.

The aim of this workshop is to propagate knowledge about transmission electron microscopy applications and to outline the advantages of transversal studies combining structural biology and cell biology. Indeed, structural biology and cell biology approaches both use TEM but are rarely merged in the same studies.

The workshop will focus on the advantages of combining both approaches, which can be easily performed with the same equipment. The workshop will focus on a biological object whose study requires such multiscale approaches: a virus. The virus will be studied in vitro to resolve its high-resolution 3D structure, and will be observed in infected cells to determine the infection and replication mechanisms in situ.

The workshop targets students and young researchers. The training will focus on a given biological object, a virus, which will be studied by two complementary approaches:

  • Single particle analysis by cryo-electron microscopy, allowing high-resolution 3D reconstruction of particles purified in vitro. This part will be performed on a 200kV TEM on the Cimex facility.
  • Cellular tomography of infected cells with observation of the virus replication sites in situ and analysis of its interaction with cellular membranes. This workshop will cover the workflow from sample preparation and resin sections realisation, to acquisition and analysis of tomograms with a 120kV TEM.

Attendees will have a theoretical and practical overview of these two complementary techniques. The practical training will be particularly emphasised, to ensure that attendees will be able to apply the knowledge acquired in the workshop for their further research projects.


Susbscription here: https://www.azur-colloque.fr/DR04/inscription/preinscription/245

Preliminary programme

The first edition of “Digital Spaces for Research and Medicine” will take place at Institut Curie on July 7th, 2022. This event will be dedicated to providing scientists and clinicians insight into Virtual Reality, Augmented Reality and Mixed Reality based tools that offer novel and powerful ways for visualizing and analysing data in research and medicine. This day will include presentations of the tools followed by hands-on demonstrations. The day will close with an open forum for questions and discussing future perspective. The following tools will be presented:

  • MorphoNet-VR (Serpico-STED, Inria-UMR144 Institut Curie and LIRMM): Visualizing live microscopy data (3D+T) in VR
  • Genuage (Team LOCCO, UMR168, Institut Curie): visualization and analysis of multidimensional point cloud data, such as single molecule data, in virtual reality
  • DIVA (Institut Pasteur, Institut Curie): 3D reconstructions of raw experimental image stacks that are integrated in virtual reality for volumetric analysis
  • Tamed Cloud (ENSADLab): immersive VR experience to interact with cloud of images
  • HoloTracks (TEAM INRAE-Unité MaIAGE / AVIZ Inria, Paris Saclay): Immersive (AR) visualization of dynamic compounds in living cells
  • UnityMol A&VR (UPR 9080, IBPC) : deep inside molecules – digital twins at the nanoscale

Presentations of the tools will be at amphitheater Burg and online (click here to join)

Registration is mandatory for hands-on demonstrations : register here to book your slot!

Program

9 – 9.15 am Welcome coffee

9.15 – 11.30 am Presentations of the tools

11.30 – 1 pm Demonstrations part 1

2 – 5 pm Demonstrations part 2

5 – 6 pm Discussion “Future and perspectives”

6pm Cocktail

GRenoble OliGomérisation GROG will cover the latest innovations
in imaging techniques and analysis methods to quantify oligomerization and
clusterization in live cells.
The meeting will mostly deal about Single molecule localization microscopy and Fluorescence fluctuation methods and will be concluded with a round table.

Les inscriptions pour la seconde édition du TED ImaBio lundi 2 mai à Montpellier sont ouvertes.

Le GDR ImaBio vous propose de venir decouvrir les dernières innovations des partenaires industriels du GDR , et pour les etudiants un focus sur les métiers du domaine.

Programme

The 7th edition of OPTOGEN will bring developers and end-users in the field of Optogenetics and Neurophotonics together. It will be a great occasion to meet in person for stimulating discussions and to reignite collaborations.

Registration and abstract submission are open.

Early registration will close on April 15th.

Thanks to the support of the IHU Foresight and of the sponsors, inscription is free for students registered before April 15th and some travel grants will be available.

Euro-BioImaging is organizing a third online User Forum on Tuesday, April 5, 2022 from 14:00-17:00 CEST. The topic is “At the Forefront of Neuroscience.”

This event will highlight the importance of cutting-edge imaging technologies in support of brain research and showcase the specific expertise available at our Nodes across Europe through case studies presented in tandem with the research community. 

In addition, keynote presentations from Hervé Boutin, University of Manchester, and Selma Ding, Max Planck Institute for Animal Behavior, will further reveal the potential of biological and biomedical imaging technologies to boost brain research. 


Register here: https://us02web.zoom.us/meeting/register/tZ0ucuytpj8iE92A-JjvPbvpNnmTF4Z7iRaC

More information: https://www.eurobioimaging.eu/news/join-us-for-the-third-euro-bioimaging-user-forum-at-the-forefront-of-neuroscience/

Programme

Deadline: April 15th, 2022

The three national infrastructures ProFi, France-BioImaging and FRISBI along with the GIS IBiSA are pleased to announce a call for a funded access to IBiSA-labelled facilities.

Our aim is to promote IBiSA facilities networking through interdisciplinary research projects.

Applications should request access to at least two different IBiSA facilities from two disciplines (structural biology, Biological imaging and proteomics). The call is open to any academic laboratory.

The amount of the financial support will be up to 5000 € per application to cover facility costs.

Applications should be submitted to: Call-IBISA-FBI-FRISBI-PROFI@i2bc.paris-saclay.fr
using the template document https://mycore.core-cloud.net/index.php/s/VNLU2jgfcP4o0NN


The deadline for this inter-infrastructure access call is 15 April 2022.

All submitted proposals will be peer-reviewed by independent experts and the final funding will be approved by a committee comprising 2 representatives from each infrastructure as well as representatives
from GIS IBISA. We advise the project PI to contact the chosen facilities in order to set-up the optimal experimental design.

Call description