Imagerie-Gif core facility, from our Ile-de-France Sud node, is pleased to announce the acquisition of a Scanning Ion Beam Electron Microscope (FIB-SEM) and a Lattice Structured Illumination Microscope (SIM) Elyra 7. For the occasion, the core facility is organizing “3D Res/volution“, a scientific event on high-resolution 3D imaging on December 15, 2022 from 2:00 pm to 5:00 pm at B21 amphitheatre. This event will be a great opportunity to introduce to you the possibilities of these 2 new systems available at Imagerie-Gif.

Free but mandatory registration: https://evento.renater.fr/survey/3d-res-volution-day-8pdzetbi

Ranging in size from 20 microns to 1 milimetre, Meiofauna is a crucial link between micro- and macro- marine ecosystems. Valentin Foulon, a research engineer in marine biology, is part of the Blue Revolution program that aims to develop a taxonomic identification protocol for these tiny creatures. He approached the Bretagne-Loire node of France-BioImaging, in Nantes, where a Single Plane Illumination Microscopy (SPIM) light-sheet available in open access helped him to further reach his goal.

Valentin Foulon loves imaging and microscopy – and he loves meiofauna. He works on the Blue Revolution program, whose goal is to accelerate the taxonomic identification of meiofauna using artificial intelligence algorithms. But while the confocal-microscopy-based protocol worked well for small plankton, the researchers quickly realized that it had to be adapted to work with meiofauna. 

Choosing the right microscopy approach

Identification of Meiofauna is usually operated manually on a classical microscope, which is “incredibly challenging” says Valentin. “And we only have one focal plane. To see important details, we need a 3D view of organisms.” explains Valentin. “That’s why we got curious about light-sheet imaging, because we would be able to see the organisms in 3D.” As France-BioImaging provides this type of microscopy in open-access in Nantes, “we clearly saw an opportunity there,” explains Valentin. His project proposal, which was submitted to the Euro-BioImaging pilot User Access fund, was fully funded by France-BioImaging, in a generous initiative introduced by France-BioImaging to fund all user projects to its facility received by Euro-BioImaging for the pilot User Access fund. 

“I had never tried light sheet microscopy before,” says Valentin, “And in Nantes, the lab didn’t have experience with marine organisms. Together, we found a compromise between different imaging parameters such as the resolution or the acquisition time.” Once the set-up was fine-tuned, he sent his samples, and the engineer at the lab did the imaging work. “It was a very nice experience,” expresses Valentin.  “I discovered a new technique, which is always enriching. I also think the lab was happy to work with a new sample type. And finally, we validated our proof-of-concept, proving that it is possible to image these organisms in 3D.

Image data management

But the project doesn’t end there. “The next step is data management. We imaged between 200-300 different organisms. It’s not enough for automatic classification, but nevertheless, we have 6-7 TB of data. Now we must process the data for this classification with machine learning. It’s an essential part of the project, to make the link between sample imaging, and data processing. We are working to close that gap.” 

“The outcome of this project is to validate a proof-of-concept, from the sample collection to the image classification. With this new taxonomic identification protocol, we will be one step forward in the comprehension of meiofauna, these mysterious marine organisms, who may hold the key to understanding the impact of human activity on marine ecosystems,” concludes Valentin. 

Thank you Marianna Childress, communication officer of Euro-BioImaging, for the original article.

SPAOM is a joint effort from Red Española de Microscopía Óptica Avanzada (REMOA) and the Portuguese Platform of Biomedical Imaging (PPBI), to organize an annual congress covering new applications in optical microscopy and image analysis.
This year’s programme offers 5 plenary sessions + 5 community workshops + numerous workshops and tech bites from commercial partners. 

REGISTRATION AND ATTENDANCE IS FREE FOR ALL PARTICIPANTS

The topics:

  • Open Microscopy, 3D/Live imaging, Super-resolution, Multiplexing/Multimodal, &  Networks/communities  (https://igc.events.idloom.com/spaom2021#section-speakers)
  • 5 community workshops: Frugal bioimaging, High-throughout/screening analysis; Machine Learning in Bioimage analysis; Phototoxicity; Remote Access…)
  • Several workshops by our sponsor partners: Agendo/Cirklo, Bruker, Zeiss | ILC, Izasa, Leica, Myltenyi Biotec, Olympus, Thermofisher  | 3i Intelligent Imaging Innovations, PhotonLines.

There will be also flash-talks, and a prize for the best communication from the submitted abstracts! The full list of speakers and scientific programme can be found here: https://igc.events.idloom.com/spaom2021

(the event will be online, through the Hopin platform, transmitted from Oeiras @IGCiencia)

Registration is here: https://igc.events.idloom.com/spaom2021/register

Euro-BioImaging is pleased to announce that the next special edition of the Virtual Pub, organized by Euro-BioImaging and the Euro-BioImaging Industry Board, will take place on December 10, 2021. In line with the season, this edition will focus on the theme of “COLD” in imaging.

They are inviting abstracts for 5 minute Flash talks about “COLD” in imaging. Be creative! Possible interpretations of “COLD” include:

  • Biology of the cold – from cold receptors and arctic organisms to brain reactions to cold and infections with common cold viruses
  • Cold-related metabolism including fat metabolism
  • Cold technologies – from cooling and freezing samples to low-temperature and cryo-imaging techniques
  • Keeping your sample or equipment cool – from reducing laser output and keeping high-power magnetic coils cool to incubating your sample at controlled low temperatures
  • Cold isotopes in molecular imaging

Please submit your abstract by November 22! https://www.surveymonkey.de/r/SpecialEditionCold

Prizes will be awarded to all speakers!

For more information: https://www.eurobioimaging.eu/news/submit-your-abstract-for-a-special-cold-edition-of-the-virtual-pub/

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Le Réseau d’Imagerie Cellulaire Paris-Saclay a le plaisir de vous inviter à une visioconférence de 2 heures portant sur le thème de l’imagerie préclinique.

Intitulé : Applications de l’imagerie in vivo dans la recherche et le développement préclinique.
Date : 21 octobre 2021
Horaire : 14h à 16h
Programme : Imagerie en transplantation du foie — Imagerie CT et PET-CT sur les modèles primate d’infection SARS-CoV-2 — Imagerie 3D des infections virales respiratoires — TEP TSPO pour l’imagerie de la neuroinflammation et du gliome.
Accès au lien Visio pour la conférence : https://www.ric-paris-saclay.fr

The FOM2021 online  conference will start on Sunday morning, March 28 at 11:30 hrs, and is preceded by free tutorials starting Sunday, 9:15 hrs. Please note that these are local times in Amsterdam which is Central European Time (CET).

Both lectures and posters will be presented “live” by the presenting author. Whole oral sessions will be recorded and be accessible by participants during and after the conference for approximately two months. Contributions will not be recorded and accessible individually.

If you wish to be kept informed on updates, please leave your email address at FocusOnMicroscopy.org/stayinformed.

Key subjects for the conference series are the theory and practice of 3D optical imaging, related 3D image processing, and especially developments in resolution and imaging modalities. The conference series covers also the rapidly advancing fluorescence labeling techniques for confocal and multi-photon 3D imaging of -live- biological specimens.

Typical topics of the upcoming FOM2021 online conference include:

  • Theory and practice of confocal and multiphoton-excitation microscopy
  • Super-resolution/ nanoscopy imaging including: PSF engineering (4pi, SIM, STED), fluorescence activation/quenching, stochastic/centroid (PALM, STORM, GSDIM, SOFI and related techniques), TIRF
  • 3D and 4D live cell and tissue imaging
  • Adaptive optics for microscopy
  • Light sheet microscopy
  • Phase/interference based microscopies
  • OCT, holographic, endoscopy
  • Advanced fluorescence imaging/spectroscopy: FRET, FRAP, FLIM, FCS, SOFI
  • New fluorescent probes, proteins, quantum dots, single molecule imaging
  • Clearing and expansion techniques
  • Coherent non-linear microscopies: SHG, THG, SFG, CARS.
  • Multi-dimensional fluorescence and Raman spectroscopy imaging
  • Correlated microscopies, like light/electron
  • Laser manipulation and tracking, photo-activation
  • Fast acquisition, automated and high-content microscopy
  • 3D image processing and visualization of multidimensional data.

Programme: http://focusonmicroscopy.org/

Registration Deadline: March 25th, 2021

Biogenouest propose une journée d’animation le 19 mars 2021 sur le thème « Symbiose et microscopie chez les microorganismes et les végétaux marins » . Les associations vont du mutualisme au parasitisme et lient intimement deux partenaires sur du long terme pour le meilleur comme pour le pire. La microscopie permet de révéler et d’étudier ces interactions au plus près des organismes, avec des techniques en pleine mutation. Cette journée leur est dédiée, écologues du milieu terrestre plus que bienvenus !

INSCRIPTION

Programme 

9:00 | TESTS

9:10 | Présentation des plates-formes de Bio-imagerie de Biogenouest par Perrine PAUL-GILLOTEAUX, plate-forme MicroPICell, Nantes
9:20 | Investigating the biodistribution of mCherry labelled Staphylococcus aureus in the porous wood material using confocal spectral laser microscopy par Muhammad TANVEER MUNIR, Ecole supérieure du bois, LIMBHA, Nantes
9:40 | Rôle des microRNA dans l’interaction plante-Rhizomicrobiome par Abdelhak EL AMRANI, UMR 6553 « Ecosystèmes, Biodiversité, Evolution » (ECOBIO), Rennes
10:00 | Régulations immunitaires impliquées dans le contrôle des bactéries endosymbiotiques et l’homéostasie de l’hôte par Abdelaziz HEDDI, UMR 0203 « Biologie Fonctionnelle Insectes et Interactions » (BF2I), Lyon
10:30 | Pause
10:50 | Apport de la microscopie photonique, électronique, tomo, CLEM pour l’étude des mécanismes cellulaires impliqués lors du processus de greffage chez Arabidopsis par Lysiane BROCARD, Bordeaux Imaging Center, Bordeaux
11:10 | Décryptage fonctionnel d’un virus mutualiste de guêpes parasitoïdes par Marc RAVALLEC & Anne Nathalie VOLKOFF, UMR 1333 « Diversité, Génomes & Interactions Microorganismes – Insectes » (DGIMI), Montpellier
11:40 | Etude du parasitisme au sein du plancton marin par des techniques d’imagerie haute résolution par Johan DECELLE, laboratoire physiologie cellulaire & végétale, CEA-Grenoble / Laure GUILLOU, UMR 7144 « Adaptation et Diversité en Milieu Marin », responsable scientifique de la plate-forme Merimage, Roscoff
12:00 | Conclusion

The webconference organized by ITMO Molecular and Structural Bases of Life (BMSV) of the Aviesan Alliance entitled “Physics-Biology interface” will take place on Thursday, December 17th 2020.

Advances in biology opened up new areas of research that are developing at the interface with physics.

Physicists make a major contribution to this interface, with the development of new instruments allowing the massive data acquisition, the manipulation of single molecules and the observation of biological phenomena at increasingly higher temporal and spatial resolutions. These developments offer new perspectives for solving previously unaffordable biological questions.

Furthermore, theoretical approaches from physics are now necessary to tackle new problems: those linked to cellular heterogeneity, to the way it is regulated, filtered or even exploited; or those linked to newly identified cell compartments, devoid of membranes and very dynamic, which are revolutionizing our vision of cellular organization and functioning.

A detailed understanding of all these complex phenomena requires new conceptual frameworks developed in physics (quantum, statistics, signal theory, thermodynamics out of equilibrium, study of polymers and colloids, etc.) which will make it possible to model living things from the atomic scale to integrated cellular systems.

This conference is therefore dedicated to the interface between biology and physics and aims to promote interactions between the two communities by illustrating the currently emerging questions and new scientific challenges.

INVITED SPEAKERS

  • Patricia Bassereau – Institut Curie – Paris
  • Martin Blackledge – Institut de Biologie Structurale – Grenoble
  • Céline Boutin – IRAMIS-NIMBE
  • Pascal Hersen – Institut Curie – Paris
  • Eric Hosy – Institut Interdisciplinaire de Neurosciences – Bordeaux
  • Arnaud Hubstenberger – Institut de Biologie Valrose – Nice
  • Martin Lenz – Laboratoire de Physique Théorique et Modèles Statistiques – Orsay
  • Marcello Nollmann – Centre de Biochimie Structurale – Montpellier

Registration deadline: December 3rd, 2020

Le programme de ce WEB-JST (Flyer téléchargeable ici) comprend quatre présentations orales (20’ + 10’ de questions) en microscopies photonique et électronique, en traitement d’image et en imagerie chimique ainsi que L’AG du réseau présentant les actions menées durant l’année écoulée.

Registration is now open for the Virtual Early Career European Microscopy Congress 2020!

Following the cancellation of emc2020, this virtual meeting will provide an opportunity for Early Career Scientists who would have attended and presented at the congress, to still present their work at an International Meeting this year. 

Registration Fees
The registration fees can be found below.

 Price
Early Career
You are a student or an Early-Career Researcher (less than 3 years since the completion of your PhD by November 2020).
Thank you to the European Microscopy Society for subsidising to enable free registration for Early Career Researchers.		FREE
EMS Member
You are a member of the European Microscopy Society. Member fees are applicable only if your membership status is active at the time of registration. Your membership status will be verified, so you must be sure that your annual subscription is current.		€60
Non-Member€75

To purchase your tickets, please visit www.emc2020.eu

For help and queries
If you have any questions regarding this event, please do email katejermey@rms.org.uk or visit the website www.emc2020.eu for further information.

The pathways leading from cells to embryo are highly complex. They involve among others steps of cell determination, patterning, cellular and organ morphogenesis, processes that are tightly controlled and coordinated in space and time.

However, during the last decade, this field has benefited from major findings in the understanding of epigenetics, gene regulatory networks, mechanobiology, mechanisms of cell specification among others, and also from technical advances such as single cell omics.

This symposium will address state-of-the-art research in this field, both in animals and plants. It will feature keynote and invited lectures, and selected short talks and posters.

The symposium is organized at the initiative of the Multi-Organization Thematic Institute Cell Biology, Development & Evolution (ITMO – BCDE), jointly with the French Society of Developmental Biology (SFBD) and with the French Society of Cell Biology (SBCF). See the composition of the Scientific Organization Committee. 

The meeting will take place as scheduled on November 16 – 17, 2020. Given the sanitary situation, it will be organized using a mixed format, partly online and partly on site, on the Institut Jacques Monod campus, located in the center of Paris.  Many short talks will be selected by the organizers (applicants will be informed around October 15th). The call for abstracts is now open. Download the submission form on the registration page. Abstract submission by October 1st, 2020.

See the invited faculty

Download the poster of the meeting.

Free registration before October 31st HERE