Correlative Microscopy (CLEM) takes an increasing value in scientific research.

The second France BioImaging course on correlative microscopies is jointly organized by Institut Jacques Monod, Institut Pasteur and Institut Curie.

The first day (free access, registration required) will be composed of a conference series from national and international speakers about the latest CLEM technologies as well as by participants who will follow the practical session in the following 4 days.

Practical training will be organized in 2 sessions of 2 days for 3 groups between the 3 organizing institutes (12 positions available).

The 2 sessions will be chosen among the 3 available:
– EMPACT2 CLEM / Tokuyasu (Institut Jacques Monod)
– Live Cell to HPF, Quick Freeze Substitution/fluorescence on sections and eC-CLEM alignment (Institut Curie)
– Live Cell to FIB-SEM / Cryo-CLEM (Institut Pasteur)

Registration on the FBI website, dead-line 31 March 2017

First day: free entrance
Practical sessions: 250.00 euros in total, selection on application form.

FBI Grand

On Friday April 14 2017, we will have the pleasure to welcome you to our Annual Meeting, to be held at the Curie Institute (Paris, France). This event, open to all members of the bioimaging community, aims to provide a platform to discuss pivotal subject matters in our field. This year, the France BioImaging Annual meeting will focus on the topic of “Future challenges in BioImaging”.

This main topic will be divided into six “challenges”, as described below:

CHALLENGES

Challenge 1: “QUANTIFICATION OF THE MOLECULAR DYNAMICS AND COORDINATION IN CELLS AND SMALL ORGANISMS, INCLUDING AT THE NANOMETER SCALE”
This session will focus on emerging in super-resolution, single-molecule and fluctuation microscopies. The session will bring together scientists working on new technological developments, on new probes, and new acquisition schemes including three-dimensional and in depth imaging. Specifically, we will aim to cover the following topics:

  • Quantification of dynamics in Super-Resolution, single-molecule, and fluctuation based microscopies.
  • Use of adaptive optics for in depth imaging in tissues or small organisms
  • New technologies for 3D imaging, including single-molecule localization and STED.
  • New probes for super-resolution and fluctuations techniques.
  • Micro-scale dynamics and molecular interactions by fluctuation techniques.
The main purpose will be to discuss and brainstorm on the key methodological challenges in this field in the near future (5 years).
Challenge 2: “IMAGING ARCHITECTURES AND PROCESSES OF LIFE, FROM MOLECULAR COMPLEXES TO MULTI CELLULAR SYSTEMS”
Imaging Microscopy to explore biological systems in space and time and across scales, including the observation of development, growth and aging of organisms, is a major challenge. This session is dedicated to future challenges in microscopy imaging to integrate molecular architecture, spatial and temporal control of gene expression, sub cellular dynamics and cell behaviors in morphogenesis and organogenesis.
Keywords: Multimodal an multiscale imaging; developmental biology; in toto 3D+time imaging; super resolution in correlative approaches; CLEM.

Challenge 3: “NEW FRONTIERS FOR IMAGING, SENSING, AND CONTROLLING BIOMOLECULES”
Methods for remote control of cellular processes by means of a trigger (light, magnetic field,…) have seen explosive growth. Recently, efforts have led to the engineering of powerful approaches in which fusing biomolecules with triggerable modules enables to turn their function on and off in response to the trigger action. Associated with intrumental tools (e.g. optical methods of active illumination or excitation beam shaping), these approaches have yielded an outstanding alternative  to standard genetic or pharmacological methods with much improved spatiotemporal resolution to analyze cell signaling and other molecular regulation in cells and multicellular complexes. On the other hand, new  probes and methods allow for visualization and sensing of molecules in live cell and during the development of multicellular organisms. This challenge should reflect the latest progresses on how the integration of chemistry and biology will frame our ways to investigate Life Sciences.
Challenge 4: “UNCONVENTIONAL IMAGING”
The combination of  imaging techniques with spatially and temporally tailored beams and reconstruction algorithms provide new approaches to cell imaging. These approaches exploit the properties of electromagnetic fields, such as polarization, spectrum or wavefront diversity, and new measurement schemes. We will discuss the implementation of such unconventional imaging techniques and the specific challenges that they face in terms of sensing, collection, data processing, and interpretation.

Unconventional imaging techniques include imaging from polarization/phase/aperture diversity, imaging using ultrafast pulses or wavefront control, imaging through turbid media

Challenge 5: BIOIMAGE INFORMATICS, IMAGE PROCESSING AND MICROSCOPY DATA MANAGEMENT”
This session will focus on emerging technologies in life science regarding microscopy, informatics & applied mathematics. The purpose is to bring together scientists working in the area of informatics and scientists working in life sciences who uses microscopy as a main tool in their research. Specifically, this session will cover some of the following themes:
– Visualization, manipulation and analysis of large multidimensional images
– Image correlation and fusion of multimodal and multiscale images
– Data mining and machine learning in biological studies from microscopy images
– Spatiotemporal Modeling and simulation of biological mechanisms
– Information Technologies for distributed computing, smart data storage and interactive image data bases.
Challenge 6: NEW BIOLOGICAL MODELS AND APPROACHES: HOW WILL THEY FRAME THE NEXT CHALLENGES IN BIOIMAGING?”
In recent years, the Life Sciences have experienced an impressive number of methodological revolutions, and many new fields of investigation have opened up. This is reflected in a diversification of our models of studies (new model organisms, plant and marine; stem cells hiPCs, IPCs…), new approaches of genetic engineering (genome editing, CRISPR/Cas), new large-scale programs (Brain imaging initiatives…) and new biological concepts (epigenetics…).
These discoveries and research directly impact or will necessarily impact current or future biological imaging technologies. The purpose of this session will be to reflect on and discuss the challenges ahead. It will allow, through the multi-disciplinarity represented within FBI and consistent examples, to define the major issues that our community will have to confront in the next 5 to 10 years.

Of the six challenges, four were selected to be part of the final program : Challenges 1, 2, 3 & 5. Challenges 4 & 6 will be represented during the poster session.

SCHEDULE & PROGRAM

Oral Sessions Schedule

Poster Session Program

CALL FOR ABSTRACTS

The call for abstracts is now closed.

 

However, you can still find specifications for your poster in the following document:

Abstract Submission & Guidelines

REGISTRATION

Registration is now closed. Thank you to all who submitted abstracts.

FREQUENTLY ASKED QUESTIONS

Who can participate?

The meeting is open to all members of the bioimaging community (within and outside the France BioImaging community) who are eager to share their thoughts, ideas and research on the challenges described above.

How can I participate?

Anyone can participate by submitting an abstract for an oral presentation as part of the sessions, or for a poster presentation.

How can I submit an abstract?

Abstracts are to be submitted at the time of registration.
Abstract Submission Guidelines
Abstract Template

Can I choose the kind of presentation I want to give?

During the registration process, one can choose whether to give an oral or a poster presentation. However, due to the limited amount of slots available for oral presentations, submissions that have not been selected to be part of a session can still be presented during the poster session. Candidates also have the option to apply for an oral presentation only, or a poster presentation only.

How much does the meeting cost?

Registration is free, and lunch and coffee breaks will be provided on site. However, travel and accommodation expenses are to be covered by participants.

LOCATION

Institut Curie, 12 rue Lhomond, 75005 Paris, France

CNRSLogoCurie

The “Next generation training in biological imaging” Symposium at Focus On Microscopy 2017, 12th of April in Bordeaux

In the frame of the Euro BioImaging PPII project, France BioImaging and its partners in the international landscape will organize for the first time at the FOM conference a symposium focused on the crucial topic of innovative trainings in biological imaging. In the last years, the training offer in terms of latest development in biological imaging has become more and more diversified and specialized, to meet the need of high level expertise in core facilities and technical support for user and the broad scientific audience. The event will gather the main European actors in biological imaging training to present and discuss training strategies, innovative training models and training organization. All FOM participants will be welcome to attend this morning parallel session. Do not forget to register to the FOM meeting, held for the first time in France.

Symposium Program: Click here
FOM General Information & Registration: FOM website

Dear colleague,
Dear FBI community,

Following the decision of the Executive Board of June 30, 2016, the National Coordination is proud to announce that the winners of the FBI Image Contest 2016 are:

1. Sébastien Mailfert – Centre d’immunologie de Marseille Luminy – Aix Marseille Université with “Dalton”

Dalton © Hugues Lelouard, Mailfert Sébastien & Mathieu Fallet CIML CNRS-INSERM-AMU
Dalton © Hugues Lelouard, Mailfert Sébastien & Mathieu Fallet CIML CNRS-INSERM-AMU
Confocal microscopy
Revealing sub-population of immune cells on small intestine by 10 colors spectral imaging

AND with “Le Saint Pierre Méditerranéen”

© Noushin Mossadegh & Mailfert Sébastien - CIML, CNRS-INSERM-AMU
Le St Pierre Mediterraneen © Noushin Mossadegh & Mailfert Sébastien – CIML, CNRS-INSERM-AMU
A l’aise comme un poisson dans l’eau, les spermatozoïdes se reposent dans leur habitat, qui ressemble à un œil, avant leur grande migration. Coupe d’un testicule de nouveau-né de souris. Marquage en immunofluorescence des noyaux cellulaires (DAPI) représentés ici en cyan. L’actine représentée ici en jaune, révèle le «squelette de la cellule» (phalloïdine marquée avec le fluorochrome Alexa-647). L’image représente 256×256µm sur 4096×4096 pixels. La coupe est d’une épaisseur de 20µm. Image de microscopie confocale sur Leica SP5 ; laser 405nm et laser blanc à 633nm ; objectif 40X, O.N. 1.25, immersion à huile.

 

2. Michael Lang – Institut Jacques Monod – ImagoSeine with “Fly Monster”

Fly monster © Orestis Falklaris & Michael Lang – Institute Jacques Monod, CNRS UMR7592 - ImagoSeine
Fly monster © Orestis Faklaris & Michael Lang – Institut Jacques Monod, CNRS UMR7592 – ImagoSeine
Multifocal confocal microscopy (spinning disk, Spinning CSU W1)
Drosophila third instar larval head, nuclear-RFP, neronal-GFP and green autofluorescence, 25x magnification, scale bar is 100 μm.

Thank you to the participants for their great contribution:

  • Sébastien Mailfert – Centre d’immunologie de Marseille Luminy – Aix Marseille Université
  • Ariane Peyret – Laboratoire de Chimie des Polymères Organiques – Bordeaux Imaging Center
  • Melina Petrel – Bordeaux Imaging Center – Université Bordeaux Segalen
  • Patrice Mascalchi – Interdisciplinary Institut of Neuroscience – University of Bordeaux – Bordeaux Imaging Center
  • Michael Lang – Institut Jacques Monod – ImagoSeine
  • Olga Nagy – Institut Jacques Monod, Drosophila Evolution Group – ImagoSeine
  • Théophile Déjardin – Institut Jacques Monod – ImagoSeine
  • Liu Zeng Zhen – Institut Jacques Monod – ImagoSeine
  • Melina Heuze – Institut Jacques Monod – ImagoSeine
  • Orestis Faklaris – Institut Jacques Monod – ImagoSeine
  • David Pereira – Laboratoire Matière et Systèmes Complexes – ImagoSeine

Thank you also to the core facilities staff and heads for having forwarded the contest to their users and for providing them state of the art Bioimaging.

The next edition of our Image Contest will open early 2017. Get ready!

The National Coordination

Dear (es) colleagues,

Registration deadline May 28, 2016
Registration: http://gdr-miv.fr/mifobio2016/pre-inscription/
September 30 to October 7, 2016
Club Belambra Seignosse, Landes
See the Poster

As you know, our school MIFOBIO on biological and technological advances in live imaging will take place in Seignosse from 30 September to 7 October 2016 (http://gdr-miv.fr/mifobio2016). This school is organized by the CNRS GDR “microscopy and imaging of living” and RTmfm network with the support of INSERM, France BioImaging, ITMO AVIESAN and EMBRC infrastructure. This year, in addition to the developmental biology models already used in previous years, this year the EMBRC infrastructure (European Biological Resource Centre Marines) will provide some biological models marine (sea urchins, sea squirts, jellyfish, sponges, etc …) to different stages of development.

This school is not reserved for experts in microscopy, but also aims to help “beyond the borders” by bringing the skills and thus provide access to the tremendous potential of imaging and microscopy for biology. To this end, we propose this year a new “challenge bio”.

“challenge bio”

Biologists, particularly those who do not usually use microscopy or wishing to test new imaging modalities can propose a biological problem for which they need imaging. It is not necessary to have a priori on imaging methods’ (acquisition, analysis and modeling) to implement. These are the people involved in imaging (acquisition and analysis) that can recognize the problems posed an interest or potential for their technical and propose experiments that will be conducted during MiFoBio.

The objective is to take advantage of the combination in one place of exceptional technological platform and cutting-edge expertise in the various disciplines, to clear new issues and pave the way for collaboration beyond school.

We invite you to register at the school through the website (http://gdr-miv.fr/mifobio2016/pre-inscription/) and if you wish to propose a “bio challenge” in imaging (localization, quantification , dynamic, interaction, multi-scale, …). Due to the large number of requests for participation at this school, selection of participants will be made on the basis of this information (300 seats).

 

cordially

Laurent Héliot, Serge Monneret, Tristian Piolot,

for the organizing committee MiFoBio

Affiche Mifobio2016 GB Dear all,

The second call for workshops in MiFoBio2016 is on!

To date, we have almost 80 workshops proposed, with 45 pre-selected. We thank all those who have brought these proposals. When selecting, a particular attention will be paid to the educational aspect, the innovative nature of the proposal, the close association of a biological problem addressed to the technological approach.

Pre-selected workshops will be validated once obtained the certainty that required material will be available during the school.
 

For this second call, we would like to reinforce certain themes, however not exclusively :

    • All type of home-made microscopy systems
    • optogenetics approaches
    • Imaging of scattering media, heterogeneous, outliers, …. (adaptive optics)
    • Application of biological models on small organisms (insects, fish, … but excluding mammals) for developmental biology
    • neuro-biological imaging
    • Photomanipulations
    • Bio-sensors
    • nonlinear imaging and non-conventional imaging (phase imaging, ….)
    • Microscopy with structured illumination
    • Contribution of microfluidics for microscopy
    • Modelling and simulation of molecular dynamics and cellular organization.

 

Procedure for proposing a workshop:

    1. Application to attend the thematic MiFoBio2016 school: go to the website of GDR-MIV: http://gdr-miv.fr/mifobio2016/ to get your identifiers.
    2. Then submit your workshop proposal via the dedicated website: http://ateliers-mifobio.fr
    3. Login using your GDR-MIV identifiers.
    4. Go in the “Workshops / Submit a new workshop” and fill in all the form fields.

In the “abstract” (1000 characters max.) Thank you to define the objective(s) of the workshop you propose. A “description” field (10000 characters) is available for a more detailed explanation. This will be used for evaluation.

The whole site is in English. Your proposals must be registered on the website before 23 April.

 

What changes from previous editions:

    • You can create and edit your workshop proposals online.
    • Workshops can have only two trainers/animators. For this purpose a specific mechanism has been put in place:

      o The main animator creates the workshop file.
      o Then goes in the “Workshops / List my workshops” section.
      o In the “Code for co-animator” he “copy / paste” the code and sends it by mail to his co-animator.
      o The co-animator connects to the site (after having pre-registred to MiFoBio). Then he goes in the “Workshops / List my workshops” section and in “Enter a co-animator code”

The workshops can be repeated two or more times during the school (the number of repetition is likely to be modulated up or down).

The workshops will be selected by the organizing committee.

The workshop team is at your disposal for any further information and can be reached at the following address
mifobio-co-atelier@services.cnrs.fr

 
The workshop team: Fabrice Cordelières, Sandrine Lecart , Christine Terryn.

MIFOBIO School 2016 pre-registration and call for Workshops are now opened !
Website: http://gdr-miv.fr/mifobio2016
Pre-registration: http://gdr-miv.fr/en/mifobio2016/pre-inscription/”
Call for Workshops: http://ateliers-mifobio.fr/
Deadline pre-registration: April 15th, 2016

Due to the growing number of demands every year, a selection process has been put in place:

  • MANDATORY pre-registration (http://gdr-miv.fr/en/mifobio2016/pre-inscription/)
  • NB: Pre-registration is only valid if you receive a confirmation email.

  • OPTIONAL submission of a workshop on the dedicated website http://ateliers-mifobio.fr
  • NB: You must be pre-registered here to access the workshop submission website.

  • Pre-registration will close on April 15th. Selection of the participants by the organising committee.
  • After selection selected person shall receive via nominative email an invitation to finalise their registration (payment).

Dear all, it is time to prepare the 7th MiFoBio School , 2016 !

Pilot committee: Serge Monneret, Tristan Piolot, Laurent Héliot.
Workshops committee: Sandrine Lécart, Christine Terryn, Fabrice Cordelières

This School is organized since 2004 by the GDR2588 within the CNRS training program, with the assistance of the vocational training unit of INSERM and supports from France-BioImaging , RTmfm and ITMO BCBDE (Aviesan). This school will take place from September 30 to October 7, 2016.
The number of places is limited to 350 participants and pre-registration will be open from February 2016 to May 2016. http://gdr-miv.fr/mifobio2016

A Call for Workshops will be launched soon !
With the support of our industry partners, but also from academic laboratories we anticipate an exceptional technology park available for you to participate and propose many practical workshops.

This year four types of workshops are solicited:

  • “Mastery” Workshops (AM): This is to illustrate the potential of a given technology to meet an identified biological questions; the experimental approach is mastered, the achievement being made on industrial devices, most often.
  • “Innovation” Workshops (AI) : the idea is to propose experiments on new technological developments laboratories (home-made experimental setups) or original operation of a set of imaging technologies
    (CLEM, multimodal microscopy, new imaging approaches, ..)
  • “Exploration” Workshops (AE): this is to experiment new approaches, onsite. For example exploring new types of samples by changing the type of analysis carried out or using other instrumental configurations, combine data obtained from different and complementary systems, in order to consider the question or biological hypothesis under several angles, make use of different analysis methods…We here encourage biologists to propose projects to explore, without insurance of results (in an exploratory way !) .The aim is to take advantage of the unique set of expertise and systems present during MiFoBio.
  • “Theoretical approach” Workshop (AT): This year we also want to foster more theoretical approaches, including modeling. We thus request the involvement of Project Leaders for such workshops, either based on real or simulated data.

The team responsible for the implementation of workshops will very soon open a registration web portal where you can propose your workshops. This first call will be closed on January 22. If necessary, a second call will be launched at the end February on more target areas to represent all school subjects, or yet to allow the improvement of certain projects presented first call requiring changes.
So if you intend to propose a workshop, it is important to get ready with your project (title, description, equipment needs, no co-host). No more than two facilitators for workshops.

FBI-AT 2016 The 3rd FBI-AT, an advanced, hands-on Training course on applications of cellular imaging to biological questions, co-organized by FBI with its genuine partner the GDR MIV, will take place in Montpellier January 18-22, 2016. One of the objectives of this 3rd session was to welcome young scientists from abroad. Among the 45 selected participants, about half foreigner colleagues and thirty PhD students and post-doc fellows will have the chance to listen and to be trained, by more than 20 experts, from seven countries. Thanks to the local organizers, we can anticipate a great success! We wish them all a productive BioImaging Advanced Training Course and an exciting meeting.

Abstract book FBI-AT 2016

an Advanced, hands-on Training course
on applications of cellular imaging to biological questions

Montpellier
January 18-22, 2016

FBI-AT 2016

Nota Bene

Website & registration:
FBI-AT 2016
Deadline registration:
October 31st, 2015
Download: Affiche FBIAT 2016

The aim of FBIAT is to show how key biological questions can be addressed with advanced cellular imaging techniques. Thus it is not only a training course on state-of-the-art imaging techniques, but additionally aims at training people on how building an experimental strategy that uses these techniques, alone or in combination, to address a specific biological problem.

In the morning, the course will have plenary lectures that introduce specific techniques and how these can solve biologically-relevant questions. In the afternoon, hands-on practicals will train attendants on these techniques, providing a complete view of the experimental flow, from sample preparation to data analysis, interpretation and, when relevant, modelization. Advanced microscopy techniques covered during the workshop include : super-resolution microscopies (PALM / STORM, Structured Illumination), Single Particle Tracking, RNA imaging, Correlation based microscopies and Atomic Force Microscopy. Trainees will choose a single module centered on their biological question of interest (see flyer)

affiche FBIAT_vf copie
Every module will have 3 practical sessions, each session being centered on a technique, plus one session dedicated to data analysis. To guaranty access to set-ups and proper training, each practical session will guest only 3 persons. The sessions will be run in parallel, such that each module will accept 8/9 participants (45 in total for the entire event).

By providing access to top training structures and lecturers, our aim to reach an international audience. Participants will thus be selected according to their previous training and adequation of the course to their needs. We will consider applications from PhD students, post-docs, staff scientists and young PIs.

logo GDR-MIV logo2 logo Univ Montpellier

FBI_logo_final_Haute resolution

From Live cell imaging to Electron microscopy

2 sessions in January and February 2016
Institut Curie, Paris, France
Correlative workflow

January & February 2016, Institut Curie
• Topics: CryoCapsule, Live cell CLEM, High Pressure Freezing, Serial sectioning, Section CLEM, Registration
• Speaker and teachers: Xavier Heiligenstein, Ilse Hurbain, Claire Boulogne, Lucie Sengmanivong, Graca Raposo
• Registration: CLOSED

Correlative Light and Electron Microscopy (CLEM) takes an increasing place in scientific investigation. In our workshop, we propose hands-on training using our innovative protocol, centered around the CryoCapsule® and our newly developed software eC-CLEM®. Practical sessions will include cell culture on the CryoCapsule® and live cell imanging, High Pressure Freezing (HPM100 and HPM Light μ), Freeze Substitution to preserve fluorescence, serial sectioning, fluorescence on section, electron microscopy and image registration.

  • It is firstly intended to scientists with advanced expertise in light microscopy and/or electron microscopy.
  • 2 sessions of 4 people each will be organized to ensure that each participant gets the chance to practice.
  • Evening discussions include presentation by each participant to have a change to discuss their own CLEM projects.
  • A practical manual will be provided, covering all the steps of the method.

FBI Grand

We will be pleased and honoured to welcome Eric Betzig during our Annual meeting 17th-18th of September, at the Institut Curie (12 Rue Lhomond, 75005 Paris).

Eric Betzig was awarded the 2014 Nobel prize in Chemistry on his work in the field of super resolution microscopy. He is going to talk about the newest developments in live cell imaging, amongst one is the revolutionary lattice light sheet microscopy technique. His talk is now scheduled within the Annual Meeting of France-BioImaging, the 17th from 11 to 12 a.m.

The scientific part of the meeting will follow the main topics below:

  • SPT-Super Resolution (WP1a)
  • Bioimage Informatics – Image Processing & Data Management (WP4)
  • HTP/HCS Imaging (WP2)
  • CLEM/Super CLEM (WP1c)
  • Inter-INBS session (invitation of Frisbi and Phenomin National Infrastructures members)
  • New Contrast & In-Depth Imaging (WP1d)
  • Multimodal & Quantitative Fluorescence Microscopies (WP1b)
  • Probe Development, Optomanipulation & Optogenetics (WP3)

* Do not hesitate to bring posters for the poster sessions (see program) !!!*

Other international invited speakers:
Dr. Rafael Carazo Salas,
Department of Pharmacology, University of Cambridge, United Kingdom
http://www.gen.cam.ac.uk/directory/rafael-carazo-salas
Prof. Clemens Kaminski,
Department of Chemical Engineering and Biotechnology, University of Cambridge, United Kingdom
http://www.ceb.cam.ac.uk/directory/clemens-kaminski
Prof. Gerard Marriott,
Department of Bioengineering, University of California – Berkeley, United States of America
http://www.marriott-lab.com/#!pi/ch5y
Dr. Erik Meijering,
Biomedical Imaging Group Rotterdam, Erasmus University Medical Center in Rotterdam, the Netherlands
http://www.imagescience.org/meijering/
Prof. Dan Oron,
Department of Physics of Complex Systems, Weizmann Institute of Science, Israel
http://www.weizmann.ac.il/complex/DOron
Dr. Yannick Schwab,
European Molecular Biology Laboratory – Heidelberg, Germany
https://www.embl.de/research/units/cbb/schwab/members/index.php?s_personId=CP-60016118

Programme FBI Annual Meeting 2015

    Registration closed

    Sorry, the FBI Annual Meeting 2015 is already full, so we have been forced to close the registration.

    Thank you for your understanding and for your interest.
    See you Next Year !

Location

CNRSLogoCurie