Service
Imaging systems for dynamic and high resolution measurements in living organisms
The Marseille node gathers two institutes in life sciences (CIML and IBDM with a shared facility: PICsL) and one in photonics (Institut Fresnel), which aim at fostering new technologies and photonic innovation for cell imaging. Founded on strong scientific and technological collaborations and dynamic industrial partnerships, the Marseille node offers a variety of state-of-the art imaging systems (e.g. multiphoton, confocal imaging, light sheet microscopy, super-resolution microscopy, electron microscopy) to a large community of scientists in the field of cell developmental biology, immunology and neurobiology. The Marseille node has a strong expertise in designing and building cutting edge optical microscopy instruments.
Key expertise and services:
- single molecule detection and fluorescence fluctuation analysis,
- ultra-resolution microscopy and nanoscopy (PALM & STORM),
- light sheet microscopy, (iv) optical manipulation (nanodissection and optical tweezers),
- polarization resolved microscopy
- nonlinear multiphotonic microscopy including 2P and 3P fluorescence, harmonic generations and coherent Raman (CARS, SRS).
2021 in Numbers
- 172 hosted projects
- 45 publications
- 10 training programs
- 9 patents since 2011
Technological Innovations
- Background free stimulated Rama spectroscopy (PRL, patent filled 2013)
- 2-Photon Lensless endoscope (Patents fille 2014-2016, Opt. Letters 2013)
- Laser manipulation of cells in vivo (PNAS 2015)
- Polarization resolved CARS (Nat. Comm., 2016)
- Polarization STORM (PANS 2016)
Tech transfer
- SRGold: Background free stimulated Raman (with APE GmbH)
Most Innovative Systems Available for Booking
- Polar resolved spinning disk
- Spot variation FCS
- Two-photon wavelength mixing and non-linear contrats (CARS-SHG-THG)
- STED 3D
- Light sheet DSLM bessel beam
- Serial Block Face – Scanning Electron Microscope
Facility
IMM Microscopy Core Facility – PICsL
Facility
InMAGIC (INMED Imaging Center) – PICsL
Facility
CIML – PiCsL
Facility
IBDM – PiCsL
R&D team
Physical approaches to cell dynamics and tissue morphogenesis team – IBDM
R&D team
Membrane dynamics and lymphocyte signaling Team – CIML
R&D team
MOSAIC Group – Fresnel Institute
Key Publications
- V. Curcio, L. A. Aleman-Castaneda, T. G. Brown, S. Brasselet, M. A. Alonso, Birefringent Fourier filtering for single molecule Coordinate and Height super-resolution Imaging with Dithering and Orientation (CHIDO). arXiv:1907.05828 Nat. Communications 11 (1) (2020) https://doi.org/10.1038/s41467-020-19064-6
- Heuke, S. Sivankutty, C. Scotte, P. Stockton, R. A. Bartels, A. Sentenac, and H. Rigneault, “Spatial frequency modulated imaging in coherent anti-Stokes Raman microscopy,” Optica 7, 417-424 (2020). https://doi.org/10.1364/OPTICA.386526
- Clara Taffoni, Shizue Omi, Caroline Huber, Sebastien Mailfert, Mathieu Fallet, Jean-Francois Rupprecht, Jonathan J. Ewbank, and Nathalie Pujol, « Microtubule plus-end dynamics link wound repair to the innate immune response“, eLife, 9, (2020) DOI: 10.7554/eLife.45047
- Sebastien Mailfert, Karolina Wojtowicz, Sophie Brustlein, Ewa Blaszczak, Nicolas Bertaux, Marcin Łukaszewicz, Didier Marguet, Tomasz Trombik, “Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells”, JoVE, 2020 http://doi.org/ 10.3791/61823
- Kaur S, Mélénec P, Murgan S, Bordet G, Recouvreux P, Lenne PF, Bertrand V. Wnt ligands regulate the asymmetric divisions of neuronal progenitors in C. elegans embryos. Development. 2020 Apr 6;147(7):dev183186. DOI: 10.1242/dev.183186 PMID: 32156756