The Normandie Node is composed of one imaging facility so called PRIMACEN (HeRacLeS, US 51-UAR 2026, with user service, research and development activities), and six highly visible R&D teams (SFR IRIB, NORVEGE, INC3M, SCALE and BB@C) expert in microscopy techniques, controls and tools. Mainly located in Rouen and distributed to Caen and Le Havre, the Normandie node is offering high level technical and innovative methodological expertise in multi-scale imaging (TEM, STED, FLIM, TIRF, 2P, LSM…) at the interface between physiology, biology, chemistry, bioimage analysis, from the molecule to the small animal/plant. The Normandie Node has expertise in vascular sciences, microalgal biosciences and intercellular communication. Moreover, we have founded the International master program in cell imaging (IMAC) where students are intensively trained on PRIMACEN equipment and have the opportunity to go abroad including Finland thanks to a tight cooperation.

2022 in numbers

  • 109 hosted projects
  • 42 publications
  • 4 training programs
  • 11 patents since 2011

Technological Innovations

  • Combination of STED and FLIM
  • Multimodal probes and new organic fluorophores
  • Quality control

Tech transfer

  • STED, FLIM and STED-FLIM combination
  • Cryomethods in sample preparation
  • Lymphangiography through LSM
  • Angiography and OCT

Most innovative systems available for booking

  • Live imaging (automated, TIRF, spinning disk)
  • Spectral confocal imaging
  • In vivo multiphoton microscopy
  • Light-sheet microscope for cleared samples
  • Macroscopy for live and fixed samples
Facility

PRIMACEN

↗ Website ↗ Service + Info

Facility: PRIMACEN

Head: Ludovic GALAS

ludovic.galas@univ-rouen.fr
PRIMACEN, CURIB, Université de Rouen, Rue Lucien Tesnière, Mont-Saint-Aignan, France

Services on this Facility

Service

Microscopy Technologies

R&D team

Endothelium, Valvulopathy, Heart Failure – Laboratory EnVI

↗ Website + Info

R&D team: Endothelium, Valvulopathy, Heart Failure – Laboratory EnVI

Head: Jeremy BELLIEN

inserm.u1096@univ-rouen.fr
UFR Santé, 22 Bd Gambetta, 76000 Rouen, France
R&D team

Bioorganic Chemistry team – COBRA lab

↗ Website + Info

R&D team: Bioorganic Chemistry team – COBRA lab

Head: Xavier FRANCK

xavier.franck@cnrs.fr
1, rue Tesnière 76821 Mont Saint Aignan cedex, France.
R&D team

Epigenetics and pathophysiology of neurodevelopmental disorders team – CBG

↗ Website + Info

R&D team: Epigenetics and pathophysiology of neurodevelopmental disorders team – CBG

Head: Bruno GONZALEZ

Bruno.gonzalez@univ-rouen.fr
22 Boulevard Gambetta, Rouen, Normandy, France
R&D team

Study of glycosylation pathways in microalgae team – GlycoMEV

↗ Website + Info

R&D team: Study of glycosylation pathways in microalgae team – GlycoMEV

Head: Elodie RIVET & Muriel BARDOR

elodie.rivet@univ-rouen.fr
CURIB, Rue Lucien Tesnière, Mont-Saint-Aignan, France
R&D team

tPA and Neurovascular Disorders – PhIND

↗ Website + Info

R&D team: tPA and Neurovascular Disorders – PhIND

Head: Denis VIVIEN

vivien@cyceron.fr
Cyceron, Boulevard Henri Becquerel, Caen, France
R&D team

Environmental Stresses and Aquatic Biomonitoring team – SEBIO

↗ Website + Info

R&D team: Environmental Stresses and Aquatic Biomonitoring team – SEBIO

Head: Céline BOULANGE-LECOMTE

frank.lefoll@univ-lehavre.fr
25 Rue Philippe Lebon, Le Havre, France

Key publications in 2022

  • Boujut M, Chevalier A, Schapman D, Benard M, Galas L, Gallavardin T, Franck X. (2022) Indazole versus indole-based cationic merocyanines with red shifted in-cellulo emission for selective mitochondria imaging. Dyes and Pigments 198, 109988. doi.org/10.1016/j.dyepig.2021.109988.
  • Brosolo M, Lecointre M, Laquerrière A, Janin F, Genty D, Lebon A, Lesueur C, Vivien D, Marret S, Marguet F, Gonzalez BJ. In utero alcohol exposure impairs vessel-associated positioning and differentiation of oligodendrocytes in the developing neocortex. Neurobiol Dis. 2022, 171:105791. doi: 10.1016/j.nbd.2022.105791
  • Faklaris O, Bancel-Vallée L, Dauphin A, Monterroso B, Frère P, Geny D, Manoliu T, de Rossi S, Cordelières FP, Schapman D, Nitschke R, Cau J and Guilbert T. (2022) Quality assessment in light microscopy for routine use through simple tools and robust metrics. J Cell Biol 221: e202107093
  • Heron C, Dumesnil A, Houssari M, Renet S, Lemarcis T, Lebon A, Godefroy D, Schapman D, Henri O, Riou G, Nicol L, Henry JP, Valet M, Pieronne-Deperrois M, Ouvrard-Pascaud A, Hagerling R, Chiavelli H, Michel JB, Mulder P, Fraineau S, Richard V, Tardif V, Brakenhielm E. Regulation and impact of cardiac lymphangiogenesis in pressure-overload-induced heart failure. Cardiovasc Res. 2023;119(2):492-505. doi:10.1093/cvr/cvac086
  • Lebas H, Guérit S, Picot A, Boulay AC, Fournier A, Vivien D, Cohen Salmon M, Docagne F, Bardou I. (2022) PAI-1 production by reactive astrocytes drives tissue dysfibrinolysis in multiple sclerosis models. Cell Mol Life Sci. 2022 May 28;79(6):323. doi: 10.1007/s00018-022-04340-z.