The GlycoMEV team (research axis 3) aims in understanding the biosynthesis and secretion of glycoproteins with a special focus on N-glycosylation in microalgae models. As in all Eukaryotes, N-glycosylation occurs in the endoplasmic reticulum and the Golgi apparatus, where numerous enzymes act step-by-step to build and transfer oligosaccharides named N-glycans on secreted proteins. To decipher the sequence and regulation of these events, it has recently developed cryopreparation methods and imaging technologies (i.e. TEM) enabling the characterization the microalgae cell morphology and to analyse the subcellular localisation of distinct molecular actors. Furthermore, the team has acquired expertise in molecular biology techniques specific to these models allowing the generation of strains expressing various proteins fused to fluorescent reporters. They have also developed strategies based on confocal/FLIM imaging to characterize the targeting mechanisms responsible for the localization of glycoenzymes in the Golgi apparatus