The translational Cardiovascular Research team develops fluorescence-based 3D imaging techniques and intravital microscopy approaches, in order to apply them for solving key questions related to the molecular and cellular functions, notably related to inflammatory responses in hearts and vessels. The researchers and technicians of the team, in collaboration with PRIMACEN, have implemented and developed set-ups for macroconfocal lymphangiography, vascular thrombosis evaluations as well as light sheet imaging, which are regularly optimized for new applications and projects. The team implemented in 2016 the first cardiac lymphangiography in rodents. Later, the team developed approaches for light sheet 3D imaging. These protocols are accessible to the community through their integration into the platform PRIMACEN (IBISA).