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Imaging and Cytometry Platform (PFIC) – Institut Gustave Roussy

Type: Facility

Business services: No

Head title: Director

Head name: Tudor MANOLIU

Contact: tudor.manoliu@gustaveroussy.fr

Website: https://www.gustaveroussy.fr/fr/imagerie-et-cytometrie-microscopie-activites

Node: Île de France Sud

Location: 114 Rue Edouard Vaillant, Villejuif, France

Available service offers on this site

Confocal microscopy

2 Leica SP8 confocal microscopes, equiped with HyD detectors and classical PMT detectors. The facility has expertise in practical training, counseling users in IF protocols, sample mounting and preparation.
3D imaging

GR dorsal chamber: implantation surgery and confocal/multiphoton imaging

Multi-modality dorsal chamber (photonics, MRI, ultrasound) enabling long-term longitudinal monitoring (several weeks). Performance (in collaboration or team training) of surgical transplants and high-resolution image acquisition using macroscopes/confocal/multiphoton microscopes.
Intravital microscopy

High-resolution imaging + EXM

Confocal microscopy or/and spinning disk microscopy combined with sample expansion technique. Boyden and al.
3D imaging Super-resolution microscopy

Holotomography (label-free, long-term imaging)

Holotomography merges holography and tomography to solve the challenge of recovering light phase shifts to generate image contrast in Quantitative Phase Imaging. HT illuminates a cell with very low power…
Label-free imaging

Intravital imaging (dorsal chamber, exposed organs)

Acquisition of images using confocal/multiphoton macroscopes/microscopes in dorsal chambers or on surgically exposed organs (without chamber).
Intravital microscopy

Intravital multiphoton imaging

3D imaging Intravital microscopy Molecular live imaging Others

Light-sheet imaging

Strong expertise in sample preparation, transparisation, handling and imaging for Lightsheet microscopy
3D imaging

Spinning disk microscopy

We are equiped with one Nikon +Yokogawa W1 spinning disk, 6 wavelenghts. We propose technics like TIRF, FRET, FRAP and LiveSR (Gataca solution). The facility has strong expertise in dynamic…
Molecular live imaging

Super-resolution imaging (STED)

STEDYCON Microscope Confocal and STED techniques all in one system. 775 STED laser + 405-488-560-640 lasers
Super-resolution microscopy

Widefield microscopy

Molecular live imaging

Widefield microscopy and deconvolution

2 Widefield microscopes are available for our users. A Leica DMI8 Video-microscope and a Leica Thunder microscope. The Thunder is equiped with 8 Led sources of excitation:390,440,475,510,555,575,635,747 nm. Two Quad…
Molecular live imaging