The scientific project of the BioImage Analysis (BIA) unit is to develop image analysis and computer vision tools for the processing and quantification of multi-channel temporal 3D sequences in biological microscopy. The topics are centered about the development of new algorithms for multi-particle tracking, deformable models, mathematical imaging and spatial distribution analysis. The group has produced powerful tools for spot detection and counting in real-time imaging of virus and genes, movement and shape analysis in 3D+t microscopy and cell growth analysis. These methods and algorithms have now been regrouped under the open-source and free platform Icy (http://icy.bioimageanalysis.org), which provides a comprehensive framework for extended reproducible research in bioimage informatics. They have been instrumental for the successful achievement of a large number of collaborative biological projects.