The team aims at developing techniques and methodologies in fluorescence microscopy to study dynamics of protein-protein interactions and biochemical activities in live sample. Team approaches are mainly driven by methodological and technological development, its transfer and its applicability in biology to answer relevant new questions. Recently, the team is investigating the possibility to implement smart and autonomous microscopy approches to be able to develop robust and automated high content microscopy pipelines of cutting-edge methods such as F-microscopy or photoperturbative experiments.