A National Research Infrastructure for Biological Imaging

Ultrapole – Pasteur

Contact: Jacomine Krijnse-Locker

ultrapole Member of CITECH (Center for Innovation & Technological Research), the Ultrapole at Pasteur provides technical and scientific support in electron microscopy, mainly, but not exclusively, to the Pasteur community, analyzing host-pathogen interactions at the ultra-structural level. Our equipment enables us to do state-of-the-art EM for life sciences that includes three dimensional EM by tomography and focused ion beam SEM. The ultrapole is run by ten members (eight engineers and two technicians), each specialized in certain techniques. We put a big emphasis on correlate light- and electron microscopy to study key (rare events) of host-pathogen interactions and we have designed several protocols to combine room temperature LM and cryo-LM with FIB-SEM, cryo-SEM and cryo-tomography.
As from the fall of 2015 the ultrapole will also have a small research group focusing on membrane acquisition of large DNA-viruses. Through this research theme we wish to establish and develop robust, new protocols for various EM-techniques.