Full pipeline support for single molecule RNA FISH
On demand from basic training for image acquisition to full pipeline support in multi modal imaging
High-Throughput Light Microscopy for Organoids: Preparation, Imaging (spinning disk; CLSM; 2-photon) and Analysis
Numerous tests to monitor the performance of photonic microscopy systems, including light source power, observation field homogeneity, detector stability, etc. Training at PRIMACEN or at the applicants’ site.
Workflow including sample preparation, 2P microscopy, confocal microscopy, light sheet microscopy, ophthalmic imaging/OCT/ERG and image analysis.
Workflow including sample preparation, light sheet microscopy, confocal microscopy, intravital microscopy, and image analysis.
Workflow from sample preparation, MET and cryo-confocal, advanced photonic microscopy
WF/confocal/FLIM, CLEM, image analysis (AMIRA)
Inscoper module for control, Nikon FNE stand, Yokogawa CSU W1 head, two cameras
Stellaris 8, WLL, FALCON, Leica Microsystems
In vivo visualization of plant tissues cellular development, as well as of their response to climate variations
(Key-words : PICPLANT facility with greenhouses (S2), 3 growth chambers for small and large plants, CONVIRON climatic chamber, histology, stereomicroscope, Spectral-FLIM confocal microscope.
Live Imaging and molecular dynamics for cell, organoids, tissue and embryo : from sample preparation with access to a L2 cell culture room, to microscopy and data analysis
(Key words : spinning disk, lattice light sheet, widefield, LightSheet, FRAP, photoconversion, FCS, spot variation FCS, TIRF, single molecule, FRET, FLIM))
Single Molecule mRNA Imaging : smFISH in 96 plates and low-troughput multiplexed smFISH in collaboration with Edouard Bertrand’ lab, from probe design and handling to data pre-processing and training for analysis.
(Keywords : smFISH, multiplexed FISH, HCS microscope, Microfluidics coupled to widefield and STORM microscope)