Hight-throughput and high content screening : from sample preparation, automation in sample preparation, data acquisition (plate reader or microscopy), image analysis and statistics analysis (list of hits)
(Key-words: chemical libraries, siRNA libraries, sgRNA CRISPR-Cas9 arrayed libraries covering entire genomes (human and mouse), automatic platform for sample preparation, HCS microscopes with low and high resolution, flow cytometer with images)
Forces (nN range) are applied or probed by atomic force microscopy, while samples are correlatively and simultaneously observed by fluorescence microscopy in time-resolved confocal microscopy, epifluorescence or TIRF. Imaging can be coupled to single molecule localisation superresolution microscopy
Measuring biomolecular structure, dynamics and interactions in vitro and in living cells. This includes distances and interactions measurements by single molecule FRET and FRET/FLIM and interactions and dynamics by FCS
3D SIM on microorganisms
We use Zeiss LSM 980 NIR with large possibilities of laser excitation. We propose panel design to acquiistion and imag analysis with QuPath
We use different imaging modalities to probe chemical fingerprinting in pharmalogical drug studies or cancer diagnosis. Many types of samples can be imaged such as liquids, powders, cells, tissues, organisms
We use multiple imaging systems to probe the polarization of fluorescent molecules. This gives an access to their molecular organization and orientation within cells and organisms.
2 Leica SP8 confocal microscopes, equiped with HyD detectors and classical PMT detectors. The facility has expertise in practical training, counseling users in IF protocols, sample mounting and preparation.
We are equiped with one Nikon +Yokogawa W1 spinning disk, 6 wavelenghts. We propose technics like TIRF, FRET, FRAP and LiveSR (Gataca solution). The facility has strong expertise in dynamic high resolution imaging. Sample preparation for long time lapse imaging.