Confocal microscopy or/and spinning disk microscopy combined with sample expansion technique. Boyden and al.
Expertise in Leica SP8 multi-position HCS confocal imaging in 98-well plates for spatial analysis of microbiological biofilms.
Acquisition of images using confocal/multiphoton macroscopes/microscopes in dorsal chambers or on surgically exposed organs (without chamber).
Multi-modality dorsal chamber (photonics, MRI, ultrasound) enabling long-term longitudinal monitoring (several weeks). Performance (in collaboration or team training) of surgical transplants and high-resolution image acquisition using macroscopes/confocal/multiphoton microscopes.
Light microscopy enables biosensors to be used to characterize the cellular environment (pH, ROS, calcium, etc.) in order to highlight differences between populations or identify heterogeneities within tissues or cells themselves. Cytometry allows multiparametric analyzes offering the possibility of measuring several activities simultaneously cell by cell. It is thus easy to compare the results obtained, to deduce their interdependence or not, which makes it an essential tool for the study of cell signaling.
In order to characterise dynamic phenomena at the molecular level, we have developed expertise in evaluating their diffusion (FRAP) and highlighting interactions (FRAP/FRET-FLIM).
The study of membrane-less compartmentalisation in cells is a growing field. At Imagerie-Gif, we have developed methods to identify these phenomena and quantify their dynamics using light microscopy techniques (DIC/FRAP/tracking, etc.).
Multimodal microscopy, by combining fluorescence, harmonic and electron microscopy signals, is therefore of real interest for taking advantage of complementary information and increasing the sensitivity of pathology detection to qualify a complex tissue state.
Ex: Imaging of stimulated Raman scattering (SRS), coherent anti-Stokes Raman scattering (CARS), and two-photon (2P) fluorescence (or autofluorescence). We will thus be able to offer multimodal analysis on a single sample section in order to obtain spatial mapping of molecular compounds of interest, such as CH2 groups for lipid analysis (steatosis assessment) or CH3 for protein analysis.
spatial super resolution by ISM (Image Scanning Microscopy) with AiryScan and NSpark in confocal (Zeiss and Nikon); by structured illumination with NSIM and RIM (Nikon and prototype); by SMLM with the STORM system (Abbelight).
High content microscopy with Nikon’s confocal LIPSI system combines automated image acquisition with integrated analysis, making it particularly powerful for high-content screening and live-cell studies. By reducing manual steps such as focusing, exposure adjustment, and data processing, it ensures reproducibility and standardization across experiments. This system is equipped with a robotic arm for scanning up to 20 multi-well plates or 80 slides on both fixed and live samples. A colour camera is also available for combining fluorescence acquisition with histological staining.
makes it possible to observe a large number of biomarkers simultaneously and to identify the diversity of cell populations and their interactions within a sample. Immunofluorescence imaging offers high spatial resolution over large regions, but is traditionally limited to a few biomarkers that can be observed simultaneously. The new so-called multiplex approaches make it possible to carry out several dozen markings on a single section of biological sample with the spatial resolution of conventional immunofluorescence. These approaches require real expertise in the design of the labelling protocol and image acquisition. The TSA-OPAL and CODEX/Phenocycler methods are based on fluorescent immunolabelling techniques using several cycles of labelling and/or observation, and are available on the MicroPICell platform. Cell Dive is available on H2P2. The 2 platforms have advanced automatic marking machines. Acquisition of new equipment is underway on PIA4 and will be availalble early in 2026.
support from sample preparation to image analysis – including visualisation of organoids/spheroids in 3D imaging. Pool of systems and expertise for these studies (transparization, staining, light sheets, etc.).