L’INRA (unité Ingenum), le PEPI MACS (Mathématiques Appliquées – Calcul Scientifique) et le Groupe de Travail Galaxy de l’Institut Français de Bio-informatique proposent une formation pour l’intégration d’outils sous Galaxy. Cette formation s’adresse principalement aux ingénieurs travaillant dans les domaines autour de l’imagerie : traitement d’images, phénotypage, reconnaissance de formes, histologie quantitative, etc.

Cette formation se déroule en deux temps. Une première journée de cours présentera les bonnes pratiques d’intégration. La seconde journée sera consacrée à l’intégration de ses outils sous Galaxy. A partir des outils à intégrer chaque participant sera en mesure de construire un/des workflows sous Galaxy.

Dates: 16 au 17 mai
Lieu : Nantes (Westotel)
Frais d’inscription HT (incluant l’hébergement et la restauration)
personnels INRA : 150 €
autres académiques : 250 €
industriels : 500 €

Clôture des inscriptions : 31 mars 2017

Pour plus d’informations : http://www.france-bioinformatique.fr/g4i

Pour le comité d’organisation : Eric Biot, Anthony Bretaudeau, Christophe Caron, Olivier Inizan, David Legland, Valentin Marcon, Nicolas Parisey

We are pleased to announce the 5th EMBL Course for Bioimage Data Analysis to be held from Sunday, 14 May – Saturday , 20 May 2017. The deadline for application is March 15th, 2017.

This course will focus on computational methods for analysing images of proteins, cells and tissues, to boost the learning process of participants who have an immediate need to analyze images in their own research. For this, the course extends from basic foundations of image processing and programming to the actual implementation of workflows using scripting in ImageJ macro and MATLAB languages. We aim to gather expert knowledge to organize a world-leading course for bioimage analysis in the fields of cell & developmental biology, biochemistry and biophysics. A report written by a student in 2014 could give you some idea about the course.

The course will take place in Heidelberg, Germany at the EMBL Advanced Training Centre. Registration and motivation letter deadline is March 15, 2017. Please visit our course website for more details.
We look forward to welcoming you to Heidelberg, Germany.

Scientific Organisers,
Kota Miura, University of Heidelberg, Germany, NIBB, Okazaki Japan
Sébastien Tosi, Institute for Research in Biomedicine – IRB Barcelona, Spain
Perrine Paul-Gilloteaux, CNRS SFR F. Bonamy University of Nantes, France

If you have any questions, please do not hesitate to contact:
Bronagh Carey
Administrative Organizer, BIAS 2017
Conference Officer
European Molecular Biology Laboratory

The Executive Board of France-BioImaging has decided to support the following projects:

AAP Support to Events
  • Fourth Edition of Super-Resolution techniques in practice – focus on two-colour PALM/STORM imaging, Marseille, March 23, 2017 – by Sébastien Mailfert (CIML). Funding by Bordeaux Node.
  • 16èmes journées de formation du RCCM : Les cryo-méthodes en microscopie électronique, Roscoff, May 22-24, 2017 – by Sophie Le Panse (Station Biologique Roscoff, CNRS UPMC). Funding by FBI Coordination.
  • 14èmes Assises Nationales des Plates-formes du réseau RTmfm, Marseille (IBDM, Parc Scientifique de Luminy), March 20-22, 2017. Funding by FBI Coordination.
  • 2nd FBI CLEM Course, Paris, May 29th – June 2nd, 2017 – by Xavier Heiligenstein (Curie Institute). Funding by FBI Coordination.
  • 5th International Frontiers in Neurophotonics Symposium – FINS 2017, Bordeaux, October 15, 2017 – by Daniel Choquet (CNRS-IINS). Funding by FBI Coordination.
  • WG2 meeting, Montpellier, January 25, 2017 – by Thomas Walter (Curie Institute). Funding by Paris-Centre Node.
AAP User Access
  • High resolution quantitative imaging of voltage-gated calcium influx in the dendrites of cerebellar Purkinje cells: identifying a mechanistic path for GIRK channels modulation of synaptic plasticity, by Pellegrino Lippiello (University of Naples – Department of Pharmacy), hosted by Paris-Centre Node (IBENS).

FBI Grand

On Friday April 14 2017, we will have the pleasure to welcome you to our Annual Meeting, to be held at the Curie Institute (Paris, France). This event, open to all members of the bioimaging community, aims to provide a platform to discuss pivotal subject matters in our field. This year, the France BioImaging Annual meeting will focus on the topic of “Future challenges in BioImaging”.

This main topic will be divided into six “challenges”, as described below:

CHALLENGES

Challenge 1: “QUANTIFICATION OF THE MOLECULAR DYNAMICS AND COORDINATION IN CELLS AND SMALL ORGANISMS, INCLUDING AT THE NANOMETER SCALE”
This session will focus on emerging in super-resolution, single-molecule and fluctuation microscopies. The session will bring together scientists working on new technological developments, on new probes, and new acquisition schemes including three-dimensional and in depth imaging. Specifically, we will aim to cover the following topics:

  • Quantification of dynamics in Super-Resolution, single-molecule, and fluctuation based microscopies.
  • Use of adaptive optics for in depth imaging in tissues or small organisms
  • New technologies for 3D imaging, including single-molecule localization and STED.
  • New probes for super-resolution and fluctuations techniques.
  • Micro-scale dynamics and molecular interactions by fluctuation techniques.
The main purpose will be to discuss and brainstorm on the key methodological challenges in this field in the near future (5 years).
Challenge 2: “IMAGING ARCHITECTURES AND PROCESSES OF LIFE, FROM MOLECULAR COMPLEXES TO MULTI CELLULAR SYSTEMS”
Imaging Microscopy to explore biological systems in space and time and across scales, including the observation of development, growth and aging of organisms, is a major challenge. This session is dedicated to future challenges in microscopy imaging to integrate molecular architecture, spatial and temporal control of gene expression, sub cellular dynamics and cell behaviors in morphogenesis and organogenesis.
Keywords: Multimodal an multiscale imaging; developmental biology; in toto 3D+time imaging; super resolution in correlative approaches; CLEM.

Challenge 3: “NEW FRONTIERS FOR IMAGING, SENSING, AND CONTROLLING BIOMOLECULES”
Methods for remote control of cellular processes by means of a trigger (light, magnetic field,…) have seen explosive growth. Recently, efforts have led to the engineering of powerful approaches in which fusing biomolecules with triggerable modules enables to turn their function on and off in response to the trigger action. Associated with intrumental tools (e.g. optical methods of active illumination or excitation beam shaping), these approaches have yielded an outstanding alternative  to standard genetic or pharmacological methods with much improved spatiotemporal resolution to analyze cell signaling and other molecular regulation in cells and multicellular complexes. On the other hand, new  probes and methods allow for visualization and sensing of molecules in live cell and during the development of multicellular organisms. This challenge should reflect the latest progresses on how the integration of chemistry and biology will frame our ways to investigate Life Sciences.
Challenge 4: “UNCONVENTIONAL IMAGING”
The combination of  imaging techniques with spatially and temporally tailored beams and reconstruction algorithms provide new approaches to cell imaging. These approaches exploit the properties of electromagnetic fields, such as polarization, spectrum or wavefront diversity, and new measurement schemes. We will discuss the implementation of such unconventional imaging techniques and the specific challenges that they face in terms of sensing, collection, data processing, and interpretation.

Unconventional imaging techniques include imaging from polarization/phase/aperture diversity, imaging using ultrafast pulses or wavefront control, imaging through turbid media

Challenge 5: “BIOIMAGE INFORMATICS, IMAGE PROCESSING AND MICROSCOPY DATA MANAGEMENT”
This session will focus on emerging technologies in life science regarding microscopy, informatics & applied mathematics. The purpose is to bring together scientists working in the area of informatics and scientists working in life sciences who uses microscopy as a main tool in their research. Specifically, this session will cover some of the following themes:
– Visualization, manipulation and analysis of large multidimensional images
– Image correlation and fusion of multimodal and multiscale images
– Data mining and machine learning in biological studies from microscopy images
– Spatiotemporal Modeling and simulation of biological mechanisms
– Information Technologies for distributed computing, smart data storage and interactive image data bases.
Challenge 6: “NEW BIOLOGICAL MODELS AND APPROACHES: HOW WILL THEY FRAME THE NEXT CHALLENGES IN BIOIMAGING?”
In recent years, the Life Sciences have experienced an impressive number of methodological revolutions, and many new fields of investigation have opened up. This is reflected in a diversification of our models of studies (new model organisms, plant and marine; stem cells hiPCs, IPCs…), new approaches of genetic engineering (genome editing, CRISPR/Cas), new large-scale programs (Brain imaging initiatives…) and new biological concepts (epigenetics…).
These discoveries and research directly impact or will necessarily impact current or future biological imaging technologies. The purpose of this session will be to reflect on and discuss the challenges ahead. It will allow, through the multi-disciplinarity represented within FBI and consistent examples, to define the major issues that our community will have to confront in the next 5 to 10 years.

Of the six challenges, four were selected to be part of the final program : Challenges 1, 2, 3 & 5. Challenges 4 & 6 will be represented during the poster session.

SCHEDULE & PROGRAM

Oral Sessions Schedule

Poster Session Program

CALL FOR ABSTRACTS

The call for abstracts is now closed.

 

However, you can still find specifications for your poster in the following document:

Abstract Submission & Guidelines

REGISTRATION

Registration is now closed. Thank you to all who submitted abstracts.

FREQUENTLY ASKED QUESTIONS

Who can participate?

The meeting is open to all members of the bioimaging community (within and outside the France BioImaging community) who are eager to share their thoughts, ideas and research on the challenges described above.

How can I participate?

Anyone can participate by submitting an abstract for an oral presentation as part of the sessions, or for a poster presentation.

How can I submit an abstract?

Abstracts are to be submitted at the time of registration.
Abstract Submission Guidelines
Abstract Template

Can I choose the kind of presentation I want to give?

During the registration process, one can choose whether to give an oral or a poster presentation. However, due to the limited amount of slots available for oral presentations, submissions that have not been selected to be part of a session can still be presented during the poster session. Candidates also have the option to apply for an oral presentation only, or a poster presentation only.

How much does the meeting cost?

Registration is free, and lunch and coffee breaks will be provided on site. However, travel and accommodation expenses are to be covered by participants.

LOCATION

Institut Curie, 12 rue Lhomond, 75005 Paris, France

CNRSLogoCurie

mmc2017 is the flagship event of the Royal Microscopical Society and presents the very best in microscopy and imaging.
Abstract Submission and registration are now open, find out more at www.mmc-series.org.uk

The mmc2017 conference will consist of six daily parallel sessions, bringing together the Frontiers in Biomaging Meeting as well as EMAG, SPM, Microscopy Society of Ireland and Scottish Microscopy Group meetings with the Congress talks, allowing the scientific programme to cover an incredibly wide range of microscopy and imaging topics and techniques.

Each day will welcome world-class Plenary Speakers, with Monday 3 July boasting two as well as a number of Pre-Congress Workshops focusing on the ‘hot topics’ of the field which will not only prepare you for the exciting week ahead but also enhance your overall experience.

By registering for the mmc2017 conference, you will also have access to the huge mmc2017 exhibition which will take place from 4 – 6 July and will house over 100 companies offering equipment and services to improve and assist in the measurement process and the many operations and procedures stemming from it. There are plenty of training opportunities throughout the free exhibition with 4 commercial workshops areas and the Learning Zone from the Royal Microscopical Society, all boasting their own daily programme to teach you new skills or build on existing ones. The exhibition is also where you can find the enormous Poster Village and the shortlisted images from the RMS Scientific Imaging Competition (which is now accepting entries.)

Both Congress Delegates and Exhibition Visitors are able to attend the mmc2017 Satellite Events taking place each day which target more specific areas and gather peers to discuss these areas and share experiences and ideas. Meetings confirmed so far are; NEUBIAS Bioimage Analyst Community Meeting, Cross Disciplinary EM and LM Meeting and the FIB & EM Prep User Group Meeting.

The “Next generation training in biological imaging” Symposium at Focus On Microscopy 2017, 12th of April in Bordeaux

In the frame of the Euro BioImaging PPII project, France BioImaging and its partners in the international landscape will organize for the first time at the FOM conference a symposium focused on the crucial topic of innovative trainings in biological imaging. In the last years, the training offer in terms of latest development in biological imaging has become more and more diversified and specialized, to meet the need of high level expertise in core facilities and technical support for user and the broad scientific audience. The event will gather the main European actors in biological imaging training to present and discuss training strategies, innovative training models and training organization. All FOM participants will be welcome to attend this morning parallel session. Do not forget to register to the FOM meeting, held for the first time in France.

Symposium Program: Click here
FOM General Information & Registration: FOM website

Reporting on the 1st international training course on “Management and Operation of Imaging Core Facilities”, Global BioImaging H2020 Project November 16-18, EMBL, Heidelberg

20 participants attended this first course, representing European and international facilities (Argentina, Australia, India, South Africa). The course was organized around 4 sessions (Soft Skills for Core Facility Staff; Administrative Skills, Quality Management, Metrology; How to set-up an Imaging Core Facility (exchange workshop); E-learning/hands-on in virtual training platform) with a strong participation of the French Community members as speakers. All sessions were overall well received, as suggested by the feedback collected via survey. Some adjustments were however proposed that should frame the next GBI Training Course (planned in Australia, summer 2018). In the meantime, GBI will also organize the 2nd workshop “Exchange of Experience” for core facility managers, 15-16 September 2017 in Bangalore, India and will continue to develop its “shadowing” program (personal exchanges between imaging facilities worldwide; second session March  – September 2017). Stay tuned! For details, read more on the Euro BioImaging website.

France BioImaging was present at the ASCB 2016 meeting in San Francisco (December 3-7).  It was a great occasion to present our infrastructure on our booth and to draw future strategies in BioImaging for Cell and Development Biology.

The France BioImaging booth at ASCB 2016
(Click the image to view)

We also interacted with scientific organizations and funders, such as the Howard Hughes Medical Institute,  the National Science Foundation and the Gordon and Betty Moore Foundation to whom we presented our R&D programs as well as the overall FBI organization. Most of them were amazed by what we could propose and surely other meetings and new programs could emerge from those informal although in depth discussions.

Beyond the above type of contacts, young scientists were attracted by FBI possibilities in terms of training capacity, accessibility to the most emerging technologies, software platform and lots of them were asking about FBI PhD and Post-doc programs. Certainly a prospect we could mine in the future.

As a general feedback on what were the active new fields of interest in the scientific area of the congress, let us mention the obvious developments in the Biology of Induced Pluripotent stem Cell (IPCs), CRISPR/Cas genome editing techniques, cell mechanics approaches and structure/function of macromolecular complexes in living cell and organisms. It is clear that FBI anticipation in developing super-resolution, optogenetics, multi-scale and highly sensitive imaging techniques will best serve our research teams focusing their interest in these diverse fields.

Finally, as many of our foreigner colleagues mentioned, attendance of French researchers was quite remarkable at ASCB this year. A number of them gave talks that were highly appreciated. A majority of them were coming from the direct perimeter of the FBI Nodes and clearly benefited from or even participated to the development of advanced technologies in imaging provided by France BioImaging.

The Executive Board of France-BioImaging has decided to support the following projects:

AAP Support to Events
  • Workshop super résolution sur échantillons végétaux, Bordeaux Imaging Center, UMS 3420, 1-2 décembre 2016 – by Lysiane Brocard, Pôle d’imagerie du végétal, domaine de la grande Ferrade INRA, Villenave d’Ornon. Funded by FBI Coordination.
  • ANF “Statistiques pour l’imagerie – STATIMAGE -”, Sèvres, 19 juin 2017 – by Claude-Marie Bachelet, IR1 Inserm. Funded by FBI Coordination.
  • 9th Imaging the Cell, Rennes, 28-30 juin 2017 – by Funded by FBI Coordination.
  • Colloque national de la Société Française des Microscopies, 4-7 juillet 2017 – by Funded by FBI Coordination.
AAP User Access
  • Identification and characterization of unknown blinking molecules present in Deinococcusradiodurans by single molecule spectral imaging, by Dominique Bourgeois (IBS, Grenoble), hosted by Bordeaux Node (BIC).
AAP Tech & Meth Transfer
  • Ievgeniia Gazo (Observatoire Océanologique de Villefranche sur mer), 3 weeks, Ile de France Sud Node (BioEmergences).

The Microscopy and Image Analysis Platform (MIAP) Freiburg invites you to a “Core Facility Management Software Workshop” (7.-8.2.2017).
It is primarily designed to meet the requirements of Core Facility managers, looking for the optimal solution, as well as team or IT members, who will have to setup such a management software in their home institutions. We also encourage major opinion leaders in the field to use this workshop as an opportunity to discuss and define the future needs in the field.

Please find the workshop program and registration form on the following webpage:
http://www.imaging.uni-freiburg.de/

NEUBIAS2020 conference is a new yearly meeting point for the BioImage Analysis community, and we, the organizers from the NEUBIAS network, are very glad to invite you to participate in the first appointment, to happen in Lisbon (Portugal) on 12-17th of February 2017, hosted and co-organized by the Gulbenkian Institute of Science.

The conference aims to promote knowledge- and technological- transfer between all the players of the BioImage Analysis Community: Life scientists, Image Analysis and Software Developers, Microscopists, BioImaging Facility staff, and most importantly: Biomage Analysts, a new profession in Life Science that NEUBIAS aims to support and promote.

In brief, the event articulates in 4 events:

  • A Training School for Early Career Investigators. 25 seats available, on selection.
    (12-15 Feb. Oeiras, Portugal). Application deadline 30/11/2016. Travel Grants Available.
  • A Training School for BioImage Analysts, 25 seats available, on selection.
    (12-15 Feb. Oeiras, Portugal). Application deadline 30/11/2016. Travel Grants Available.
  • A Taggathon (working meeting of NEUBIAS to develop repositories and benchmarking), on invitation.  (13-15 Feb. Oeiras, Portugal)

AND

  • A BioImage Analysis Symposium: 15-17th February, 2017 – in Lisbon, Portugal.

OPEN TO REGISTRATION NOW!

The Symposium aims to offer a broad view over the latest developments and updates in Bioimage analysis, including a Showcase giving exposure to Open software packages and tools updates, companies products, community round tables, career path debates and community progress report.
Bioimage Analysis will be covered as a broad field of Research, Technology-development and Service-for-Data-Producers (i.e. researchers in Life Science) in the context of many types of BioImage data: Optical Microscopy, Electron Microscopy, Medical Imaging, among others.

Please visit our webpage for more information: www.neubias.org

We hope to attract many of you to Lisbon in February.

On behalf of all NEUBIAS members

Gaby Martins (Event Host, co-organizer)
Sebastian Munck and Arne Seitz (Event Co-organizers)
Jean-Yves Tinevez (Training School co-Organizer)
Fabrice Cordelières and Paulo Aguiar (Training School Organizers)
Perrine Paul-Gilloteaux, Chong Zhang, Sébastien Tosi and Graeme Ball (Taggathon Organizers)
Julia Fernandez Rodriguez (STSMs coordinator)
Kota Miura (Vice Chair, Training School co organizer)
Julien Colombelli (Chair)

More About NEUBIAS: Mobility Grants, Careers, Action.
NEUBIAS is a network Action funded by COST (www.cost.eu), aiming to maximize the impact of advances in imaging technology on the Life Sciences, and to boost the productivity of bioimaging-based research projects in Europe. The Action intends to provide a stronger identity to Bioimage Analysts by organising a new type of meeting fostering interactions between all stakeholders (i.e. NEUBIAS 2020).

NEUBIAS also provides mobility funds for scientists willing to expand their knowledge in Bioimage Analysis and/or to develop Image Analysis capabilities for their research project. Check the next Call for Short Term Scientific Missions, closing on November 15th, 2016. http://eubias.org/NEUBIAS/mobility-grants/

NEUBIAS also needs the input from the whole community to devise best-practice guidelines for the career path of BioImage Analysis, a new profession still not fully recognized in the field. If your work activity embraces Bioimage Analysis to support Life Science (as a service, collaboration etc…), please take 5 minutes to fill our new “Career Consultation”: http://eubias.org/NEUBIAS/careers/

The BIC is setting up in a brand new space

In the last weeks of October 2016, the BIC has settled in a brand new building, constructed by the Regional Council of Aquitaine as part of the Neurocampus project. This building, of around 13 000 m2, is shared with the Interdisciplinary Institute for Neuroscience (IINS) and the Institute for Neurodegenerative Disorders (IMN). This building, constructed in two years, cost 47 M€ and is part of a large project to develop Neuroscience and imaging in Aquitaine. The new building is conveniently located and connected by footbridges between the Magendie Neuroscience center and the Center for functional genomics (CGFB) that hosts several core facilities.

buildingbic1

In total, the BIC will occupy 1000 m2, split between the CGFB and the new building. The major part in the new building is dedicated to photonic microscopy. Electron microscopy instruments, including two brand new ones coming in 2017, will be dispatched between the CGFB and Neurocampus building. In these new spaces, users have access to a culture room and also a room with analysis stations. Other rooms are dedicated to each kind of microscopy (one room for live cells imaging, one room for multiphoton, one room for confocal, one room for new scanning electron microscope etc…). Special rooms are dedicated to host R&D projects as well as confidential collaborations with industry.

Development of training capacities at the BIC – joint projects with the Cajal School of Neuroscience

buildingbic2

The BIC has engaged for many years in active training programs for imaging at all levels (beginners to advanced training) for local, national and transnational users. The BIC personnel also participates extensively to various theoretical and hands on training/showcase activities in France and abroad (MifoBio, NeuBias, etc…). Within the strategy to develop the BIC-FBI training, we are engaging a partnership with the Cajal Advanced Neuroscience Training Program to develop special ima ging training for Neuroscience. The Cajal school is a European FENS and IBRO initiative in partnership with Bordeaux Neurocampus and the Champalimaud Foundation, which offers state-of-the-art hands-on training courses in neuroscience.

Construction of a light sheet microscope for super resolution imaging inside living samples

Fast and non-damaging imaging of single molecules inside live organisms is essential to study physiologically relevant biochemical mechanisms occurring at the subcellular level. For example, the dynamic organization of transmitter receptors at the membrane of excitatory neurons should, ideally, be studied in vivo in the brain of animal models. Unfortunately super resolution techniques such as PALM1, STORM23 and uPAINT4 are mostly restricted to the sample external surfaces and are unable to image inside live samples.

For these reasons the Bordeaux Imaging Center is developing a new light sheet microscope specially dedicated to image single molecules into live samples. Light sheet fluorescence microscopy (LSFM) is recognized as the method of choice to image thick live samples. Compared to other fluorescence imaging modalities such as wide field, confocal, structured illumination, two-photon or STED, LSFM strongly reduces out of focus fluorescence, decreases photobleaching and phototoxicity, and improves temporal resolution. Among the numerous technical implementations of LSFM 5, we decided to build a lattice light sheet microscope (LLS) because it has been specifically designed to perform super resolution imaging in thick live samples 6. Indeed In LLS the illumination beam is shaped by a spatial light modulator (SLM) to produce a < 1 µm thick excitation plane over a length of  > 50 µm at the sample. A 1.1 NA detection objective ensures efficient light collection required for high localization precision. Illumination and detection objectives are both long working distance and water immersion, thus allowing observation of live samples up to 5 mm in diameter. (Fig 1 A)

Our LLS microscope is mostly based on the documentation freely and kindly shared by Eric Betzig’ group (HHMI Janelia Farms, USA).

fig1
Photo Credits: Mathieu Ducros

Fig 1. (A) The sample is placed at the intersection of the excitation and detection objective optic axes in a temperature controlled perfusion chamber. It is held at the tip of motorized arm on a 5 mm diameter cover slip (from 6). (B) The LLS microscope under construction in June 2016. (C) In blue and green the optical path of the excitation and detection beams respectively (from 6). A higher efficiency SLM, higher QE camera should improve the light budget compared to the original specifications. In addition, a targeted laser beam (red) will allow precise photo-conversion of light sensitive molecules.

We made a few modifications compared to the original specifications of the LLS as described in 6 : our microscope will be equipped with a laser combiner including 4 high power lasers at 405 nm (300mW), 488 nm (1 W), 560 nm (2 W), 642 nm (2W), a higher efficiency SLM (Fourth Dimension DD QXGA) and a sCMOS camera with improved quantum efficiency (Hamamatsu ORCA Flash V2). These improvements should mitigate the weak throughput of the LLS beam path, and, in turn, improve molecule localization precision and/or time resolution. In addition, a targeted photostimulation beam will be coupled through the detection objective to photo stimulate or photoconvert with a high spatial and temporal resolution photosensitive molecules.

STORM, PALM and PAINT imaging modalities will be fully compatible with the constructed LLS.

The microscope construction by Mathieu Ducros, INSERM research Engineer on the BIC, started in April (Fig 1B). First images are expected by the end of 2016. Once our LLS is fully operational and running, it will be accessible to all BIC users under the supervision of a local engineer.

For this project we are supported financially by the GIS IBiSA, LABEX brain and FBI.

References

  1. Betzig, E. et al. Imaging intracellular fluorescent proteins at nanometer resolution. Science 313, 1642–1645 (2006).
  2. Rust, M. J., Bates, M. & Zhuang, X. Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM). Nat. Methods 3, 793–795 (2006).
  3. van de Linde, S. et al. Direct stochastic optical reconstruction microscopy with standard fluorescent probes. Nat. Protoc. 6, 991–1009 (2011).
  4. Giannone, G. et al. Dynamic superresolution imaging of endogenous proteins on living cells at ultra-high density. Biophys. J. 99, 1303–1310 (2010).
  5. Santi, P. a. Light sheet fluorescence microscopy: a review. J. Histochem. Cytochem. 59, 129–138 (2011).
  6. Chen, B.-C. et al. Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution. Science (80-. ). (2014). doi:10.1126/science.1257998

 

Bordeaux Imaging Center: http://www.bic.u-bordeaux.fr/

UMS 3420 CNRS-Université de Bordeaux, US4 INSERM

Contact: bic[at]u-bordeaux.fr

Photo Credits: www.bordeaux-neurocampus.fr