Euro-BioImaging is organizing a third online User Forum on Tuesday, April 5, 2022 from 14:00-17:00 CEST. The topic is “At the Forefront of Neuroscience.”

This event will highlight the importance of cutting-edge imaging technologies in support of brain research and showcase the specific expertise available at our Nodes across Europe through case studies presented in tandem with the research community. 

In addition, keynote presentations from Hervé Boutin, University of Manchester, and Selma Ding, Max Planck Institute for Animal Behavior, will further reveal the potential of biological and biomedical imaging technologies to boost brain research. 


Register here: https://us02web.zoom.us/meeting/register/tZ0ucuytpj8iE92A-JjvPbvpNnmTF4Z7iRaC

More information: https://www.eurobioimaging.eu/news/join-us-for-the-third-euro-bioimaging-user-forum-at-the-forefront-of-neuroscience/

Programme

Euro-BioImaging-User-Forum-program_March-7Download

Deadline: April 15th, 2022

The three national infrastructures ProFi, France-BioImaging and FRISBI along with the GIS IBiSA are pleased to announce a call for a funded access to IBiSA-labelled facilities.

Our aim is to promote IBiSA facilities networking through interdisciplinary research projects.

Applications should request access to at least two different IBiSA facilities from two disciplines (structural biology, Biological imaging and proteomics). The call is open to any academic laboratory.

The amount of the financial support will be up to 5000 € per application to cover facility costs.

Applications should be submitted to: Call-IBISA-FBI-FRISBI-PROFI@i2bc.paris-saclay.fr
using the template document https://mycore.core-cloud.net/index.php/s/VNLU2jgfcP4o0NN


The deadline for this inter-infrastructure access call is 15 April 2022.

All submitted proposals will be peer-reviewed by independent experts and the final funding will be approved by a committee comprising 2 representatives from each infrastructure as well as representatives
from GIS IBISA. We advise the project PI to contact the chosen facilities in order to set-up the optimal experimental design.

Call description

Access-call-inter-infra-IBiSA-2022Download

The France-BioImaging LSFM workgroup is pleased to announce an INSERM workshop on Light-Sheet Fluorescence Microscopy imaging technics. 

This workshop will be divided in two parts:

  • A first theoretical part in Bordeaux from the 16th to the 18th of May 2022 which will cover basic principles, applications and challenges of LSFM imaging through seminars,
  • A second practical part along June 2022 where you will have the possibility to choose the set-up on which you want to be trained amongst many systems available within the France-BioImaging community.

If you want to discover, learn and/or deepen your knowledge about this vast and powerful family of 3D imaging technics, this training is for you!


You will find all the information (program, registration details, venue and accommodations, …) about this event on the following poster and website (Atelier 268): https://evenium-site.com/site/atelier-de-l-inserm-268

REGISTRATION DEADLINE: March 4, 2022

Programme

Programme-268Download

Poster

Affiche-268Download

Understanding how development is coordinated in multiple tissues and gives rise to fully functional organs or whole organisms necessitates microscopy tools. Over the last decade numerous advances have been made in live-imaging, enabling high resolution imaging of whole organisms at cellular resolution. Yet, these advances mainly rely on mounting the specimen in agarose or aqueous solutions, precluding imaging of organisms whose oxygen uptake depends on ventilation.

Engineers from the Institut Curie and Institut Jacques Monod implemented a multi-view multi-scale microscopy strategy based on confocal spinning disk microscopy, called Multi-View confocal microScopy (MuViScopy).

MuViScopy enables live-imaging of multiple organs with cellular resolution using sample rotation and confocal imaging without the need of sample embedding. They illustrated the capacity of MuViScopy by live-imaging Drosophila melanogaster pupal development throughout metamorphosis, highlighting how internal organs are formed and multiple organ development is coordinated. They foresee that MuViScopy will open the path to better understand developmental processes at the whole organism scale in living systems that require gas exchange by ventilation.

3D fusion reconstruction of eight different angles at 10x magnification. Animation of a 3D reconstruction after fusion of images acquired from eight angles by the MuViScope of an Ecad:3xGFP Drosophila pupa at 28 hAPF with a 10x objective. The animation starts with a 180° rotation along the A-P axis and then sequentially shows the eight different angles from a top view: 0° (red), 45° (orange), 90° (yellow), 135° (green), 180° (light blue), 225° (dark blue), 270° (purple) and 315° (pink). Fusion was performed using Huygens Fuser (SVI) and 3D visualization with Imaris software. The acquisition parameters are detailed in Table S1. Scale bar: 200 μm.

Olivier Leroy, Eric van Leen, Philippe Girard, Aurélien Villedieu, Christian Hubert, Floris Bosveld, Yohanns Bellaïche, Olivier Renaud; Multi-view confocal microscopy enables multiple organ and whole organism live-imaging. Development 15 February 2022; 149 (4): dev199760. doi: https://doi-org.insb.bib.cnrs.fr/10.1242/dev.199760

The MuViScope was co-funded by France-BioImaging.

Le Réseau d’Imagerie Cellulaire Paris-Saclay organise en avril prochain une visioconférence de 2 heures portant sur le thème de l’imagerie des organoïdes.

Titre : Pipeline Organoïdes de A à Z : Fabrication, Transparisation, Acquisition et Analyse d’images.

Thématique : Le RIC Paris-Saclay vous propose une sorte de pipeline sur l’imagerie des modèles organoïdes qui vous permettra de mieux appréhender l’ensemble du processus de leurs fabrications, leurs modes de transparisation, l’analyse des images 3D associées à ce type d’échantillon épais, et pour finir, l’importance de l’étude de ces modèles chez l’homme pour développer de nouveaux outils thérapeutiques.

Date : 14 avril 2022
Horaire : 14h à 16h

Programme : Auto-organisation cellulaire en systèmes 3D par imageries optiques — Tissue clearing : Adapted protocols for small samples — Analyses d’images et reconstruction 3D — Applications en Recherche translationnelle (Organoïdes hépatiques humains).

Inscription gratuite et obligatoire sur www.ric-paris-saclay.fr

Programme

Programme-version-31-marsDownload

Affiche

RIC_affiche_organoides_14avril2022_Version-30-mars-12h20Download

The thematic institute of molecular and structural basis of life sciences of the French National Alliance for Life Sciences and Health (Aviesan) has identified cellular (cryo) electron microscopy as a priority area to be developed in the future. To better understand the functional behaviour of living organism, it becomes increasingly important to describe its structural organization from atomic scales to integrated, multi-component biological systems. Recent developments in imaging have drastically transformed our perception of the molecular organization and of the dynamic interactions of molecules in cellular systems.

That is why, they are organizing a 2-days meeting entitled “Symposium on recent developments in cellular electron microscopy”, and dedicated to the latest progresses in the field of cellular electron microscopy at the interface with fluorescent microscopy and structural biology. The meeting is built around 4 scientific sessions, namely volumetric imaging, cryo-electron tomography, correlative light and electron microscopy, and new technological developments. The meeting is for molecular and cellular biologists to have an overview of the latest results and concepts that develop at this interface.

Due to the sanitary situation, vaccination pass and face masks are mandatory to attend the meeting.

Download the provisional program

programme_EM_16032022Download

ORGANIZING COMMITTEE

Yves Gaudin, I2BC

Carine Giovannangeli, AVIESAN

Bruno Robert, CEA

Patrick Schultz, IGBMC

France-BioImaging is launching a call to have up to two new Nodes joining the infrastructure.

In 2022, France-BioImaging gathers 21 biological imaging facilities and 54 R&D teams specialized in biological imaging (see https://france-bioimaging.org/). It is structured in 6 local Nodes (Bordeaux, Bretagne-Loire, Ile-de-France-Sud, Marseille, Montpellier, Paris) and one transversal Node dedicated to bioimage informatics (IPDM). France-BioImaging is also the French node of Euro-BioImaging ERIC (see https://www.eurobioimaging.eu/).

The objectives of France-BioImaging are to promote the dissemination of the latest advances in all technologies and methods related to biological imaging, and their adoption by the users of the facilities that constitute the infrastructure.

In order to expand its technology and expertise portfolio and to provide the best possible geographical coverage to life scientists across France, France-BioImaging invites node candidates to submit their letter of intent here before May 31st, 2022.

Node candidates should:

  • Be made up of imaging facilities and R&D teams specialized in biological imaging, not necessarily grouped on the same campus but with a territorial logic.
  • Demonstrate readiness to provide open user access. The facilities must be open to the outside world beyond the regional level, i.e. to the national and international levels. Each Facility must have a valid IBiSA label, or must be in the process to have their integration in an already existing IBiSA facility validated in 2023, or must obtain this label in 2023. Each facility should have a structured organization and cost accounting. The facilities must have a reservation website for access to the equipment and a user training program. The facilities will be required to have or commit to a data management plan.
  • Gather R&D teams with a high degree of innovation and demonstrate a willingness to collaborate with the facilities. They must explain and demonstrate their technology and/or know-how transfer methods, particularly towards the local platforms, but not exclusively.
  • Bring real added value in relation to the current status of France-BioImaging, whether in terms of technologies, methodologies, scientific themes, novel user communities,… (for more information, visit the FBI website and consult the service offer). This is a key criteria.
  • Have an integrated and effective governance model.

More information on the selection criteria, expected commitments and documentation that needs to be provided can be found here.

Node selection will be done via a two-step process:

  • a letter of intent (deadline May 31st, 2022): the form to be completed is available here
  • a full application (deadline September 30th, 2022).

Applications will be reviewed at both steps by an International Board and decisions will be communicated at the end of 2022.

Submit your letter of intent here

If at any time of the application process you have questions, please feel free to contact the France-BioImaging coordination Team by sending an email to contact@france-bioimaging.org.

We are looking forward to receiving your letter of intent!

Explore the beauty of the invisible world through the 2022 FBI digital calendar!

Enjoy the diversity of microscopy techniques, models and applications represented, one image at a time.

All 12 images used for this calendar were submitted to France-BioImaging image contest 2021. A big thank you again to all the participants!

You can download the A4 print version (one month per page) 2022 FBI digital calendar here:

France-BioImaging-digital-calendar-2022-vf-1Download

If you wish to use it as your computer desktop, you can download a PNG version of each month here:

January-22Download
February-22Download
March-22Download
April-22Download
May-22Download
June-22Download
July-22Download
AugustDownload
September-22Download
October-22Download
November-22Download
December-22Download

The France BioImaging Team wishes you a happy new year and all the best for 2022!

May 2022 bring new microscopy breakthroughs that will take biological research to new depths!

To start this new year, here is a beautiful & festive fluorescence microscopy image illustrating skeletal muscle fibers (Desmin, green) in co-culture with motor neurons (SMI-32) forming active Neuromuscular Junction in a cell culture method using murin primary cells in vitro. Nuclei are stained by DAPI (blue).

This image was submitted by A. Ghasemizadeh, from the Institut NeuroMyoGene, to France-BioImaging Image Contest 2021.

France BioImaging and all the French community aims to develop and promote innovative imaging technologies and methods. But microscopy images can also take an artistic, creative look and make the invisible world beautiful, allowing people to see the visual appeal of the life sciences. 

We enjoyed the diversity of the images submitted with many different microscopy techniques, models and applications represented. A big thank you to all the participants!

The National Coordination Team and the Executive Board are proud to announce the winners of the FBI Image Contest 2021:

  • 1st Place: Léna Meneux, Eye Team, Institut des Neurosciences de Montpellier

“The eye of the storm”

Sensory fibers of a mouse cornea imaged with a confocal microscope. The corneal nerves converge toward the centre forming a vortex. This particular transgenic mouse model allows stochastic expression of fluorescent proteins, unravelling the heterogeneity of the fiber origines inside the corneal epithelium.
Acknowledgements to Karine Loulier for the mouse model and Laetitia Hudececk for her help during the acquisition.

Confocal microscopy

  • 2nd Place: Eunice HoYee Chan, Muscle Dynamics Team, Developmental Biology Institute of Marseille (IBDM)
Myofibrils isolated from Drosophila indirect flight muscle labelled with titin (yellow) and actin (blue). Image captured from confocal microscope. We are studying the role of titin protein in muscle mechanics and organisation during development

“Sarcomeric bouquet”

Myofibrils isolated from Drosophila indirect flight muscle labelled with titin (yellow) and actin (blue). Image captured from confocal microscope. We are studying the role of titin protein in muscle mechanics and organisation during development.

Confocal LSM880
  • 3rd Place: Camille Boutin, Biology of multiciliated cells Team, Developmental Biology Institute of Marseille (IBDM) & Nicolas Brouilly, PICsL Imaging facility, Electron Microscopy department
Lamellar structure in a differentiating multiciliated cell observed by transmission electron microscopy with a Tecnai G2 200kV FEI.

“Clown”

Lamellar structure in a differentiating multiciliated cell observed by transmission electron microscopy with a Tecnai G2 200kV FEI.

Transmission Electron Microscopy, Tecnai G2 200kV FEI

Congratulations to the winners!

Explore all the images submitted here:

Fluorescence microscopy image illustrating skeletal muscle fibers (Desmin, green) in co-culture with motor neurons (SMI-32) forming active Neuromuscular Junction in a cell culture method using murin primary cells in vitro. Nuclei are stained by DAPI (blue).
Embryonic murine lacrimal gland (E17), with fluorescent staining showing Actin, Fibronectin, phospho-MLC and nuclei. The acquisition was performed using a confocal microscope Zeiss LSM 980, with a 25X air objective.
Confocal microscopie picture of a Drosophila larva's gut. Cyan: nuclei. Magenta: reporter for the activity of the protein GCN2. Yellow: the symbiotic bacterium Lactiplantibacillus plantarum. L. plantarum is a symbiont of Drosophila. We showed that it can communicates with its host by stimulating the activity of GCN2 in enterocytes. GCN2 may then trigger a physiological change of the gut that promotes larval growth despite malnutrition.
Representation of PALM technology on a rat hippocampal neuron. The low resolution image corresponds to the synaptic marker, Homer-GFP. Using PALM superresolution technology, it is possible to visualise NMDAR trafficking and clusters in high resolution. This approach can be used in the study of autoimmune neuropsychiatric diseases that disrupt both the organisation of NMDAR and their diffusion at the neuronal surface.
Neuronal spheroids of rat primary neurons are grown in hydrogel templates. The cells are electroporated with GFP and tdtomato. Then the spheroids are fixed and imaged with a confocal microscope. Cells in 3D structure mimic better functions and morphology properties of in vivo.
Myofibrils isolated from Drosophila indirect flight muscle labelled with titin (yellow) and actin (blue). Image captured from confocal microscope. We are studying the role of titin protein in muscle mechanics and organisation during development
The image shows the movements of Dopamine Receptors at the surface of hippocampal neurons and was acquired on a spinning disk. We took 1000 frames at 50ms exposition before reconstructing the trajectories of single receptors with PalmTracer. This allow us to understand, down to the nanometric scale, how the interaction between receptors modulate their surface diffusion.
Phages from vibro bacteria - Transmission electron microscopy
Patchwork of 4 maximum projections with different LUTs of a z-stack acquired in 2-photon microscopy. Neurons express the GCaMP6f calcium sensor following a viral infection of an organotypic mouse brain slice.
Negative staining artifact. Transmission electron microscopy.
In plants, the male gametes are represented by the microscopic round structures called the pollen. The anthers are the flower organs that produce and encase the developing pollen. This confocal microscope image is the cross-section of an anther of Arabidopsis thaliana. The developing pollen can be seen inside the anther. These are the haploid round cells containing protective pollen wall on their surface. The pollen are surrounded by diploid cell layers of the anther which nourish the young pollen supporting their development. This image was taken with the aims to understand how the growing pollen communicate with the surrounding nourishing tissues.
Aedes albopictus (Mosquito) leg imaged with a scanning electron microscope and digitally colored.
Deuterosomes producing centrioles during multiciliated cells differentiation.
Lagerstroemia sp. (Crepe Myrtle) blossom imaged with a stereomicroscope and digitally colored.
TEM observation of extracellular-vesicles from bacteria
PD1 membrane receptors super-resolution imaging in a PD1 stably expressing Jurkat cell. This acquisition has been performed with our soSPIM (single-objective Selective Plane Illumination Mocroscopy) system combined with an Adaptive Optics system (MicAO, Imagine Optique). The whole volume is composed of 35 Z-sections acquired every 500 nm, with each section consisting of 22,500 frames for a total acquisition time of 13h handled fully automatically. On each frame every molecule was precisely localized in 3D through an astigmatism-based process. The final cell exhibited more than 300,000 localizations and was 10 µm wide. To assess the spatial organization of clusters on the cell membrane, we used their centroids to compute a Voronoi diagram on the sphere approximating the cell shape.
Rat hippocampal neuron filled with a fluorescent marker and observed by dSTORM. The image shows details of dendritric spine morphology as well as surrounding axons in which we can observe the membrane-associated periodic skeleton.
Lamellar structure in a differentiating multiciliated cell observed by transmission electron microscopy with a Tecnai G2 200kV FEI.
Multiciliated cells at the surface of Xenopus embryo (Scanning Electron Microscopy)
A triple-negative cancer cell navigating its path through a dense 3D collagen matrix network. Collagen is labeled in green; Actin in magenta, and plasma membrane structure, caveolae, component in red. The image was acquired through a spinning disc confocal microscope for a 3D migration time-lapse experiment.
Ageratum bloom imaged with a scanning electron microscope and digitally colored.
Transmission electron microscopy observation of cells infected with Yersinia pseudotuberculosis in order to see their intra-vacuolar localization.
Two salivary glands from Drosophila simulans larva at L3 wandering stage. They have been stained with DAPI and rhodamine phalloidin. The upper gland is reconstructed in 3D on Imaris, nuclei and cells volumes are represented with a color scale from blue to red.

As stated in the Terms & Conditions of the contest, foreign participants non-affiliated to a French institution are featured in the gallery, but were not evaluated as part of the contest.

Euro-BioImaging first open call for user projects is open! If you have an idea for a biological or biomedical imaging project that you, your student or your close colleague could carry out in one of Euro-BioImaging Nodes, including France-BioImaging, now is the time to make this project come true with financial support from the Euro-BioImaging Access Fund.

How it works:

Submit your project proposal through the Euro-BioImaging web portal between October 20 and December 15, 2021, and indicate that you want to apply for the Euro-BioImaging Access Fund in order to be considered for a grant of up to 5.000 Euros to access the imaging services at a Euro-BioImaging Node. Projects will be evaluated by a committee of independent reviewers. Successful applicants will be notified by late January 2022 and successful projects should be started before July 2022.

What the funding covers:

The Euro-BioImaging Access Fund covers the user’s travel and accommodation costs as well as access and consumable costs at the imaging facilities that are part of Euro-BioImaging Nodes. For remote access projects shipment costs are also covered. Each successful applicant is eligible for up to 5.000 Euros of support.

Who is eligible:

All academic scientists, regardless of gender, nationality, home institution, career phase, or field of interest, are eligible to apply. We strongly encourage early career researchers to apply for this grant.

Projects should include transnational access to a Euro-BioImaging Node, i.e. the applicant’s home institution is in a different country than the Node where the project is to be performed. Due to the current sanitary situation, projects with non transnational access are elligible but transnational access will have priority.

All the external users/collaborators of France BioImaging facilities/labs are eligible:

  • any users from outside the institutional perimeter of France BioImaging nodes (i.e. from outside the following institutions: Aix-Marseille Université, Université de Montpellier, Université de Bordeaux, Université de Nantes, Université de Rennes 1, Université Paris-Saclay, Université de Paris, Université PSL -Paris Sciences & Lettres-, Généthon, Ecole Polytechnique, Institut Pasteur) who would like to use imaging technologies in one of FBI nodes: Paris Centre, Paris Ile-de-France-Sud, Marseille, Montpellier, Bordeaux, Bretagne-Loire. They can be French or international users – EU and non-EU
  • or users from a France BioImaging regional Node who want to access an equipment available in another FBI regional node.

Evaluation:

All applications will be evaluated for scientific excellence by a committee of independent reviewers. Selected projects will be assessed for technical feasibility and if needed receive technical advice from the Node providing the service.

How to apply:

Applicants are invited to visit our website to discover the range of technologies provided by Euro-BioImaging Nodes. Applicants will then follow the user access process described here: https://www.eurobioimaging.eu/about-us/how-to-access and indicate that they wish to apply for the Euro-BioImaging Access Fund in the application form.

The detailed procedure to apply to access one of France BioImaging facilities is available here: https://france-bioimaging.org/access/

Full details: https://www.eurobioimaging.eu/about-us/funding-user-access

Questions? Contact us!