Dear All,

The deadline for application to the Jacques Monod Conference on “Optical imaging of brain connectivity: from synapses to networks in action” is extended to March 14, 2016.

Don’t hesitate to send an abstract, there are still some possibilities to attend. Note that up to 6 participants will be selected for a short talk, based on their abstracts.

Best regards,
Christophe Mulle
Fritjof Helmchen

Laure BAILLY-CUIF (Gif sur Yvette, France) Imaging neural progenitor cells dynamics during behavior
Haruhiko BITO (Tokyo, Japan) Labeling, monitoring and manipulating active ensembles
Alain CHEDOTAL (Paris, France) Development of new imaging methods to study the organization of sensory systems
Daniel CHOQUET (Bordeaux, France) Nanoscopic organization of synapses
Rosa COSSART (Marseille, France) Imaging ripple events in the awake mouse hippocampus
Valentina EMILIANI (Paris, France) Two-photon optogenetics by spatio-temporal shaping of ultrafast pulse
Andreas FRICK (Bordeaux, France) Neuronal circuits probed with recombinant rabies virus technology
Viviana GRADINARU (Pasadena, USA) Brain control with light; development and application to mental disorders
Fritjof HELMCHEN (Zürich, Switzerland) Imaging cortical circuit dynamics in behaving mice
Sonja HOFER (Bazel, Switzerland) Imaging function and structure of the visual system
Anthony HOLTMAAT (Geneva, Switzerland) Neural circuits in the mammalian neocortex
Ehud Y. ISACOFF (Berkeley, USA) Design of novel probes for the optical detection and manipulation of neuronal signaling
Jinny KIM (Seoul, South Corea) mGRASP for mapping connectivity at multiple scales
Arthur KONNERTH (Tum, Germany) Impaired neuronal network function in mouse models of Alzheimer’s disease
Troy MARGRIE (London, United Kingdom) Sensory processing in single cells, circuits and behavior
Hannah MONYER (Heidelberg, Germany) Connectivity in the entorhinal cortex
Valentin NÄGERL (Bordeaux, France) Super-resolution imaging of spine plasticity
Elly NEVIDI (Cambridge, USA) Visualization of synapse assembly and disassembly in vivo: multispectral tracking of distinct circuit elements
Thomas OERTNER (London, United Kingdom) Controlling the strength and lifetime of synapses with light
Julie PERROY (Montpellier, France) Imaging plasticity at synapses
Dmitri RUSAKOV (London,United Kingdom) Mapping nanomolar calcium landscapes inside neurons and astroglia with FLIM
Angus SILVER (London, United Kingdom) Investigating the role of temporal coding using high speed 3D 2-photon imaging
Mark SCHNITZER (Stanford, USA)Development of fiber-optic fluorescence microendoscopy for studies of learning and memory
Scott STERNSON (Ashburn, USA) Neural processes that underlie hunger studied with reverse engineering of neural circuits
Katrin WILLIG (Göttingen, Germany) In vivo STED microscopy of the living mouse brain
Claire WYART (Paris, France) Investigation of a novel sensory interface relaying information from the cerebrospinal fluid to motor circuits
Hongkui ZENG (Seattle, USA) Large scale analysis of mouse brain connectivity

The spirit of this Summer School is inspired by the most prestigious school ever founded in France, Saint Flour, the influence of which has spread to generations of researchers in Mathematics. Since its establishment in Brittany in 1994, this school has become a worldwide reference. It is resolutely international (participants from more than twenty countries have participated to the previous editions) and accessible to young scientists. It is an open yet privileged place for exchanges and discussions of major on-going work. Informal and warm, at a location where the sea and the land combine in a time varying relation, this school brings together, every two years for ten days, the world great teachers and researchers in Biomedical Imaging.
Lectures, seminars, and discussions are organized at the highest level, but with the freedom of spirit that is the tradition of Brittany. The school objective is to contribute without any exclusion to advances in a rapidly evolving field, and to foster participation in the adventure of research. It provides up-to-date, state-of-art knowledge on emerging areas and addresses important issues dealing with complex, multivariate systems, going from basic to applied research.

The annual Meeting of ELMI is an internationally recognized meeting on Light Microscopy combining lectures and hands-on industry workshops on cutting edge microscopy techniques and innovative applications in life sciences. In 2016 it will take place in Debrecen, Hungary, from the 24th to the 27th of May. In addition to plenary scientific lectures, ELMI 2016 offers a fantastic opportunity for users to enjoy the hands-on workshops in the latest developing instrumentation, and the chance to gain insight into the next generation of technologies. It also provides an excellent opportunity for networking with the industry and other devoted microscopists.

The Education Department of the Institut Pasteur will be running a symposium on the same topic on Tuesday March 8, 2016, with the “Principles and Applications of Fluorescence Microscopy” course directors : David DiGregorio, Gael Moneron and Jost Enninga. This event will include a series of seminars to explore the latest technological developments in fluorescence microscopy. The symposium, which will take place in the auditorium of the François Jacob building, represents a unique opportunity to hear from the leading experts in fluorescence microscopy about their latest research findings. It is open to all scientists on campus. The event is free but prior registration is required.

The school aims at providing the researchers the numerical tools enabling the acquisition, quantification and the modelling of multimodal and multi-scale biophysical data. The school will include introductory courses on spectroscopies and image acquisition methods together with conventional processing methods. They will be followed by advanced courses on recent data processing methods, such as compressed sensing, and the illustration of their applications in different fields such as mass-spectrometry, NMR and different imaging methods. Theoretical courses will be completed by extensive practical labs on personal computer.

Softwares: Ipython Nump/Scipy and ImageJ
Registration deadline: October 12th 2015
Registration cost: 450 Euros including food and accomodation.
Poster presentation opportunity.
Audience: PhD, post-doc, researchers and technical staff.

La Mission pour l’Interdisciplinarité du CNRS et les réseaux CALCUL, DEVLOG, RBDD, RCCM, REMISOL, RTMFM organisent le 15/12/2015 à Paris une journée d’exposés et de rencontres autour du traitement d’images en microscopie.

Cette journée traitera de l’ensemble du cycle de vie des images, au vu des évolutions techniques majeures récentes. En effet, le travail aux interfaces entre différentes disciplines est une des clefs pour lever les verrous technologiques.
 
Le programme s’articulera autour des 4 thèmes suivants :

T1 : Workflow de traitements sur big data : extraction d’information, gestion de flux de tâches, fouille de données, codes de calcul
T2 – Génie logiciel: Quel logiciel (développement sur mesure ou utilisation de solutions existantes) ? Comment développer, valider, diffuser ?
T3 – Infrastructures de calcul et de stockage adaptées aux données images de microscopie
T4 – Outils et méthodes d’enrichissement sémantique des images (quels standards de métadonnées et/ou ontologies pour le partage, l’indexation/le référencement de l’information ?)

Symposium on Super Resolution Microscopy, Functional Imaging, Correlative Light Electron Microscopy (CLEM), High Throughput & High Content Screening, BioImage informatics – Image Processing and Data Management, Training, Dissemination & Technological Transfer

 

The strength of this meeting lies in the mixture of scientific lectures on state-of-the-art, high-end microscopy combined with “hands-on” workshops and exhibition of the latest technology, organized by the leading companies in the field. This year we will maintain the format and focus on leading edge developments and its implementation to life science.

The goal of this workshop is to help mobilise and liven up the interest of theoreticians and modellers and present them with the most recent experimental results and new functional questions brought about by correlation (FCS, FCCS,…) or supra-resolution microscopies (STORM, PALM…), related to how biomolecules or vesicles move in the cell and its membranes. Our objective is to promote interactions between experimentalists and theoreticians/modellers working on the spatial or spatiotemporal dynamics of: + the transcriptional machinery + synaptic receptors + viral assembly + intracellular transports

OMICS International, (conference series) the World Class Open Access Publisher and Scientific Event Organizer is hosting “International Conference on physics” which is going to be the biggest conference dedicated to Physics. The theme “Highlighting innovations and challenges in the field of Physics” and it features a three day conference addressing the major breakthroughs, challenges and the solutions adopted. The conference will be held during June 27-29, 2016 at New Orleans, USA.

Programme:

• Astrophysics and Cosmology
• Atomic, Molecular and Optical Physics
• Plasma Physics
• Chemical physics
• Particle physics & Phenomenology
• Nuclear physics
• Applied Physics
• Computational Physics and Numerical Simulation
• Mathematical Physics
• Condensed Matter Physics

Dear friends of imaging,

The BioAxial company and the Imagopole (the imaging platform of the Institut Pasteur) has collaborated for several years around the development of an entirely new approach to super-resolution imaging based upon conical diffraction. It is now a microscopy system, available commercially (http://www.bioaxial.com/products/codim100/product-description), the CODIM100.

We host such a system, and it will be made available at the Imagopole to all scientists, following the Imagopole access policy (as any regular system) by the end of September 2015. The CODIM100 is very simple to use, and can be operated by autonomous users, following a short training session.

We organize an open day for the system Thursday the 24th of September. You are cordially invited to bring your sample and try the CODIM100. We will be there all day to help you setting up the microscope to harvest the best images.

If you are interested in coming to this open day, please write to Jean-Yves Tinevez (tinevez@pasteur.fr) and state your needs. We will allocate a slot depending on the time you need. We have the facilities to host and prepare live samples.

If you are interested in super-resolution microscopy, keep reading on.
While there already exist several super-resolution modalities, this system is distinguished by the unique combination of several performance features. In brief,:

• it is ideal for live-cell super-resolution imaging (even long term time-lapse imaging);
• it works with any fluorescent sample without any special preparation;
• it has a very good sensitivity even to weakly labelled samples.

It is attached to a normal laser scanning confocal microscope. The typical usage is to image a sample with normal LSCM mode, then draw a ROI over the acquired image to rescan it in super-resolution. Here are some quick specifications of the super-resolution module:

• Excitation lines: 488, 561 and 640nm
• Objectives: 40x 0.95 air / 60x 1.2 water / 60x 1.49 oil.
• Acquisition speed: a few seconds for a 25µm2 ROI.
• Labels: any that can be excited with the wavelength mentioned above
• Sample prep: any confocal or live sample with 0.17mm glass bottom coverslip. No restrictions on mounting media for fixed samples.
• XY resolution: 110 nm with 488nm laser/0.95 NA, 90 nm with 488 nm laser/1.20 NA
• Very low excitation power: less than 1 µW/µm2.

Some examples:

New trends in super-resolution optical microscopy: unraveling the ultra-structure and dynamics of molecular assemblies in cells and tissues

PHASE I
September 30 to October 2, 2015
Bordeaux

Website – Affiche – Inscriptions

 
This Workshop will present the theoretical basis, the latest developments and the acquisition and data analysis procedures on super-resolution microscopy techniques used to investigate the ultrastructure of molecular assemblies and their dynamics in living cells and tissues.

Registration Deadline: March 27, 2015
Organizers: Maxim DAHAN (Institut Curie, Paris), Orestis FAKLARIS (Institut Jacques Monod, Paris) and Marcelo NOLLMANN (Centre de Biochimie Structurale, Montpellier)
 

Phase I : critical assessment

• Introduction to super-resolution techniques
• Application and new developments in single-molecule super-resolution microscopy
• Labeling, Theoretical and computational methods
• In-depth super-resolution microscopy, STED, SIM microscopies

Phase II : technical workshop

18 trainees will be selected among phase I participants.
 

Renseignements et inscriptions
Pôle des Ateliers – Inserm
ateliers@inserm.fr
Tél : 33(0)1 44 23 62 03 ou 04