The France-BioImaging CLEM working group is pleased to announce the organization of the next CryoCLEM Workshop, to be held from October 14 to 15, in Bordeaux.
This workshop is the ideal event to be introduced to, and to gain knowledge of, the cryoCLEM technology, with the aim of applying it to your research project.
It will be composed of a theoretical part, presented by experts in this technology, and a practical part covering the entire cryoCLEM workflow (from sample preparation to correlative microscopy in cryogenic conditions).
Banner picture copyrights: images 1 & 3 R. Anger, image 4 P. Lapios, image 2 N. Pied
The first meeting of the FBI Mechanobiology WG will take place on the 2 and 3 of December 2024 at the Centre for Integrative Biology (https://cbi-toulouse.fr/fr/) in Toulouse..
The programme will include a seminar by Matthieu Piel (Institut Curie/Institut Pierre Gilles de Gennes), presentations by participants and practical mechanobiology workshops (a choice of 3 workshops from a dozen: optical tweezers, bioprinting, microfluidics, AFM, Brillouin, finite element modelling, mechanical confinement, force measurements, etc.).
Registration is free, but places are limited and priority will be given to contributors whose abstracts have been selected.
The choice of workshops will be made at a later stage.
Preliminary Programme:
Monday, December 2nd
1pm: Welcome and seminar by Matthieu Piel
2-6pm: workshops
6pm: poster session and buffet
Tuesday, December3rd
9am-12pm: presentations by participants (and coffee break)
12pm: buffet
1.30-4pm: workshops
Call for abstracts
Abstract submission open until 15/09
TYPES OF PRESENTATIONS
Oral presentations: If you are selected for an oral presentation, you will have 15 minutes, including discussion (12 minutes presentation + 3 minutes Q&A).
Posters: If you are selected to present a poster, you will present your work at the dedicated poster session on December 2nd, afternoon.
Selected contributors will be notified at the end of September.
Registration/Abstract submission form
Advanced microscopy workshop in Bordeaux from November 4th to 7th, 2024.
This advanced training course aims to (1) present the theoretical foundations, (2) clarify and synthesize the various existing approaches to both sample and instrument preparation, and (3) provide an overview of solutions for handling and processing the data acquired. These objectives will be addressed through the prism of two important biological fields of application: Neuroscience and 3D Cell Cultures. Indeed, the versatility of light sheet methods means that questions in these two fields can be addressed at a wide range of scales, from the whole brain or organoid, to the study of the nervous system of small living organisms or brain slices, right down to the single molecule inside spheroids. To address these themes, we will draw on the expertise of the Bordeaux FBI site, whether in neuroscience or in the growth and imaging of 3D cell cultures.
The course will be structured around 4 main thematic tracks, addressing the issues of sample preparation and data analysis for given samples. Participants will have the choice of following one of these tracks, or navigating between them according to their interests. The tracks will be :
P1: Large sample imaging – Clearing & Expansion
P2: 3D cellular models Culture & Imaging
P3: Neuronal network imaging
P4: Image Analysis
The format of the course will include lectures and seminars in the morning, providing a theoretical grounding in the various areas covered (sample preparation, imaging, image processing) and presenting the latest developments in these fields, and practical workshop in the afternoon on the various sites of the Bordeaux node (IINS, BIC, VoxCell).
Ihssane Idrissi / Rémi Galland (Interdisciplinary Institute for Neurosciences, Bordeaux, France)
Vincent Studer (Interdisciplinary Institute for Neurosciences, Bordeaux France)
Gustavo de Medeiros (Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland)
Georges Debrégeas (Jean Perrin Laboratory, Paris France)
Thai Truong (University of Southern California, Los Angeles USA)
Angela Getz / Mathieu Ducros (Bordeaux Imaging Center, Bordeaux France)
Alexandra Fragola (Institut des Sciences Moléculaires d’Orsay, Orsay France)
Emmanuel Faure (Laboratory of Computer Science, Robotics and Microelectronics, Montpellier France)
Johannes Roos (Johannes Kepler University, Linz Austria)
Philippe Girard (IJM, Paris, France)
Carole Siret (CIML, Marseille, France)
Guillaume Maucort (BIC, Bordeaux France)
Workshops on
Whole brains imaging by Ultramicroscopy
3D imaging of neuronal expanded samples by Ultramicroscopy
3D entire small animal imaging
3D Cellular models culture and imaging using the soSPIM technology
Micro-niche creation for 3D cell culture and 3D imaging using the HS-ISM technique
Neurospheres culture and imaging using the MuViSPIM
Brain slices imaging using a Lattice Light Sheet Microscope
Single Cell electroporation for Brain slices labelling
Functional neuronal network imaging in ZebraFish
Orchestrating complex bioimage workflows using the Arkitekt solution
Napari for 3D data handling
How to segment a 3D dataset in just a few clicks?
Organizing committee
Coordinators: Mathieu Ducros & Rémi Galland
& FBI Work Group on « Multiscale Light-Sheet Microscopy »
Sponsors
On November 20th and 21th 2024, we have the pleasure to invite you to our Annual Meeting, to be hosted by our brand new FBI Alsace Node at the Bibliothèque Nationale et Universitaire (BNU) de Strasbourg (6 Pl. de la République, 67000 Strasbourg).
Registration is now open! Fill out the form below to register.
We will be happy to celebrate yet another year of achievements and developments in bioimaging with all the members of the community.
With a focus on “Live functional imaging: From chemical synthesis of the probes to instrumentation”, this edition aims to bring together chemists, microscope builders and biologists developping tools to probe life.
The scientific sessions will explore the development of new probes (organic, inorganic, nanoparticles, fluorescent proteins, hybrid materials), how to leverage their optical properties (spectra, quantum yield, lifetime, photostability, switching between dye states, etc…) and new instrumentations taking advantage of these new probes for life science.
France-BioImaging Mission Officers, Technology WGs, facilities or R&D teams are invited to present news, innovations or any achievements to the community with a poster. We strongly encourage you to submit an abstract for a poster presentation during your registration!
Registration is free but mandatory. Please note that the BNU capacity is limited and registrations will be accepted on a first come first served basis.
As part of the scientific activities that it wishes to carry out and in continuation of its 1st seminar (Dec. 2022), the FBI “Preclinical Microscopy” Working Group is organizing its 1st webinar on February 1 from 2:00 pm to 4:00 pm. The objective of these scientific meetings is to present research projects, technologies and regulatory frameworks related to light microscopy from the organ/organoid to the living animal.
Program
2:00 pm – Welcoming & Introduction
2:15 pm – “Visualization of the ticking of circadian clock cells in freely moving mice” by Xavier Bonnefont (Institute of Functional Genomics, Montpellier).
3:00 pm – “Regulatory aspects of the use of animals for scientific purposes” by Isabelle Bardou (Cyceron, Biomedical Imaging Platform, Caen).
On December 13th and 14th 2023, we have the pleasure to invite you to our Annual Meeting, to be hosted by our brand new FBI Toulouse Node at the Centre de Biologie Intégrative of the Université Toulouse 3 – Paul Sabatier.
We will be happy to celebrate yet another year of achievements and developments in bioimaging with all the members of the community.
This year, the Annual Meeting will focus on “Multiscale mechanobiology of cells and cell systems“, a topic specially selected for being one of Toulouse node’s expertise.
Mechanobiology aims to apply biophysical approaches to measure and perturb forces in complex physiological systems, and to develop in vitro systems including different types of organoids, enabling the controlled manipulation of cells and tissues and the measurement of mechanical forces to study cell mechanics and morphogenesis.
This “mechanobiology” theme is including (i) how cells generate and transmit forces, (ii) the impact of forces on cell/tissue dynamics, (iii) the impact of extrinsic forces on cells and tissues, and (iv) the development of new tools for manipulating and measuring forces in cells and tissues in a controlled, non-invasive way.
We invite the France-BioImaging Community to present their mechanobiology-related projects during the second day of the Annual Meeting around a dedicated session with selected talks. We strongly encourage you to submit an abstract for a talk or a poster presentation during your registration!
The winner of the best talk and the best poster presentation will win their registration fees for one 2024 microscopy related event of their choice!
Core facilities will also have an opportunity to present a poster.
En venant de Bordeaux, contourner Toulouse par l’Est (direction Montpellier) ; avant le péage prendre la sortie no 23 direction Rangueil ou la no 19 direction Ramonville, puis suivre Université Paul Sabatier.
En venant de Montpellier, après le péage, prendre la sortie no 19 direction Ramonville, puis suivre Université Paul Sabatier.
Une navette et le tramway relient l’aéroport et le centre ville de Toulouse (Transports en communs)
De là, il est possible de prendre le métro (lignes A ou B) pour rejoindre la station « Ramonville St Agne » (ligne B, direction Ramonville) : environ 1h de trajet
Le CBI est à 500 mètres à l’Ouest de la station et accessible à pieds ou en bus.
Il est également possible de prendre un taxi depuis l’aéroport : compter au moins 1/2h de trajet hors heures de pointe
En train
Depuis la Gare SNCF Matabiau:
Prendre le métro LIGNE A direction Basso Cambo, changer à la station “Jean Jaurès” et prendre la Ligne B pour rejoindre la station « Ramonville St Agne » : comptez environ 40 minutes depuis la gare.
Le CBI est à 500 mètres à l’Ouest de la station et accessible à pieds ou en bus.
Hôtels conseillés :
Nous vous conseillons de privilégier des hôtels en centre-ville.
Prise en charge des missions:
Se rapprocher de votre noeud FBI (fonds mission), sauf pour les intervenants qui seront directement contactés pour la prise en charge de leur missions.
France-BioImaging and all the French community aims to develop and promote innovative imaging technologies and methods. But microscopy images can also take an artistic, creative look and make the invisible world beautiful, allowing people to see the visual appeal of the life sciences.
We enjoyed the diversity of the images submitted with many different microscopy techniques, models and applications represented. A big thank you to all the participants!
The National Coordination Team and the Executive Board are proud to announce the winners of the FBI Image Contest 2023:
1st Place: Laurent LE, Lévêque-Fort Team, Institut des Sciences Moléculaires d’Orsay
“In the blink of an eye”
COS7 fixed cell. Alpha-tubulin labeled with DNA-PAINT and imaged with Atto 647N. Axial information is obtained by virtual-SAF measurement known as DONALD.
SMLM Fluorescence Microscopy with DNA-PAINT with DONALD detection
2nd Place:Gonzalo QUIROGA-ARTIGAS, Team Contrôle cytoplasmique de la stabilité du génome, Centre de recherche en Biologie Cellulaire de Montpellier
“Tardigrade embryos protected by mother’s molt”
Tardigrades commonly align the time of molting with egg laying. In this image we observe a tardigrade molt covering three developing embryos (DNA in white). The microscopy technology applied was confocal microscopy, and the research aimed to investigate the synchronization of embryo development in tardigrades.
Confocal microscopy
3rd Place:Hugues LELOUARD, Gorvel team, Centre d’Immunologie de Marseille Luminy
“Intestinal octopus”
Small intestine section from a LyzM-eGFP mouse containing one Peyer’s patch and stained for proliferative cells (Ki-67, yellow), Paneth cells (UEA-I, blue), epithelial cells (EpCAM, magenta), naive B cells (IgD, red), T cells (CD3, orange), helper T cells/macrophages (CD4, cyan), phagocytes (CD11c, turquoise), monocyte-derived phagocytes (GFP, green).
10-color spectral confocal microscopy
Congratulations to the winners!
Explore all the images submitted here:
As stated in the Terms & Conditions of the contest, foreign participants non-affiliated to a French institution are featured in the gallery, but were not evaluated as part of the contest.
The FBI Working Group Correlative Light-Electron Microscopy is organizing a workshop!
This workshop will take place at the Bordeaux Imaging Center (FBI Bordeaux node) from January 31st to February 2nd, 2024.
Correlative Light and Electron microscopy (CLEM) increases our capacity of biological investigation. By combining light microscopy and electron microscopy, this complementary approach takes advantages of both techniques. Light imaging provides valuable functional information thanks to its labeling power, whereas Electron microscopy excels at high resolution.
The Bordeaux Imaging Center has developed several workflows such as In-resin fluorescence and Array tomography. Both helps to determine 3D ultrastructure of a targeted area or a whole sample at different resolutions. In the framework of the FBI CLEM workshop, participants can choose one of these 2 different practicals, In-resin fluorescence and Array Tomography, in which a local specialist will show them the workflows.
Workshop 1: “In-Resin Fluorescence” – From HPF to tomography, by way of freeze-substitution and on-section fluorescence observation.
Workshop 2:“Array tomography” – From serial section to acquisition with confocal and SEM.
It is firstly intended to scientists with expertise in electron microscopy, who are expected to use the chosen technique.
2 workshops of 4 people each (you can apply only to one workshop)
A practical manual will be provided, covering every step.
You want to attend this workshop? Please fill the following form before December 22nd, 2023:
The candidates whose research projects suit the workshop the best will be selected. We will get in touch with you after the Christmas break to confirm your participation at this workshop.
This form is currently closed for submissions.
Nous vous invitons au premier webinaire sur l’Initiative Commune Afrique-France pour l’Imagerie Biologique qui se déroulera le 6 décembre 2023 à 11h00 CET!
En coordination avec l’African BioImaging Consortium et Africa Microscopy Initiative et dans le cadre du programme Horizon Europe, l’Initiative Commune Afrique-France pour l’Imagerie Biologique vise à étendre et renforcer les collaborations entre collègues africains et français intéressés par l’utilisation de microscopie avancées pour leurs propres projets de recherche. C’est dans cette optique que nous avons lancé deux appels à projet : l’un favorisant l’accès aux plateformes de bioimagerie de France-BioImaging, l’autre consistant à un programme de jumelage.
Ce webinaire sera notamment l’opportunité d’en apprendre plus sur les projets sélectionnés et sur les bénéfices de cette initiative pour les scientifiques africains et français.
Webinaire
The France-BioImaging Image Contest is back for its 5th edition!
This image contest is open to all within the imaging community: core facility staff and users, R&D labs teams and co-workers, students… Submit your best microscopy images for a chance to showcase your skills, research and creativity to the French bioimaging community and beyond, allowing people to see the visual appeal of the life sciences. Images from the contest will be featured on France-BioImaging communication tools, online and in print.
France-BioImaging and all the French community aims to develop and promote innovative imaging technologies and methods. But microscopy images can also take an artistic, creative look and make the invisible world beautiful.
We are all eager to see your work !
Prizes
1 to 3 images will be awarded depending on the quantity and quality of the entries submitted. France-BioImaging will cover the registration fees for one 2024 microscopy related event of the winners’ choice (FOM, ELMI, EMC, COMULIS conference, etc.).
Important: Only French or foreign participants affiliated to a French institution can enter the contest. Foreign participants non-affiliated to a French institution can submit images and will be featured in the gallery, but will not be evaluated as part of the contest.
Submission deadline: Friday, November 10th, 2023, 23h59 UTC+2.
We are happy to announce our 8th France-BioImaging Annual Meeting! Happening on December 13th and 14th 2023, this year’s edition will be hosted by our new Toulouse node at the Centre de Biologie Intégrative (Toulouse, France).
The Annual meeting will highlight France-BioImaging’s development and perspectives. Imaging scientists and users from the infrastructure’s nodes will present their key projects and demonstrate how they have benefited from France-BioImaging and its community.
More information about the program and the registration coming soon!
France BioImaging and all the French community aims to develop and promote innovative imaging technologies and methods. But microscopy images can also take an artistic, creative look and make the invisible world beautiful, allowing people to see the visual appeal of the life sciences.
We enjoyed the diversity of the images submitted with many different microscopy techniques, models and applications represented. A big thank you to all the participants!
The National Coordination Team and the Executive Board are proud to announce the winners of the FBI Image Contest 2022:
1st Place: Carole SIRET, Van de Pavert Team, Centre d’Immunologie de Marseille-Luminy
“Little Monster”
The embryonic formation of lymph nodes, small organs essential for the immune response, is now known. Using light sheet microscopy, scientists were able to determine the dynamics at work in this 13.5-day-old mouse embryo. In blue, the lymphoid cells (LTi), derived from the haematogenous endothelium, a specific tissue of the embryo. They pass into the liver where they proliferate before migrating through the body to give rise to lymph nodes. The 3D information obtained thus makes it possible to follow the interactions of lymph nodes with their environment, in particular with nerve cells, in green, and blood vessels, in white. The lymphatic endothelial cells and some macrophages are visible in red.
Lightsheet Microscopy
2nd Place:Magalie BENARD, Plateforme de Recherche en IMAgerie CEllulaire de Normandie (PRIMACEN), Research infrastructure HeRacLeS, Inserm US 51, CNRS UAR 2026,
“The communication link with others”
Image of a cellular interconnection between two human tumor cells whose cytoskeleton has been labeled with anti-tubulin (ATTO-647N), anti-vimentin (AlexaFluor594) antibodies and with Phalloidin probe (AlexaFluor488). Scale bar 1µm.
Confocal microscopy
3rd Place:Frédéric FERCOQ, Parasites et Protistes Libres (PPL), Museum National d’Histoire Naturelle
“Sepia”
Stage 25 cuttlefish embryo (Sepia officinalis) observed under a confocal microscope. The cuttlefish was cleared and the tissue autofluorescence was captured.
This image was produced in collaboration with Laure BONNAUD-PONTICELLI and Luis MOLINA from the BOREA laboratory.
Confocal microscopy
Congratulations to the winners!
Explore all the images submitted here:
As stated in the Terms & Conditions of the contest, foreign participants non-affiliated to a French institution are featured in the gallery, but were not evaluated as part of the contest.
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