Recap of FBI-AT 2024: “Light-Sheet Microscopy: Principle and Applications to Neuroscience and 3D Cell Culture”
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Category: FBI Special Events
From November 4 to 7, the Broca Center (Neurocampus Bordeaux) hosted the fourth edition of the advanced microscopy training organized by France-BioImaging Work Group on « Multiscale Light-Sheet Microscopy ».
FBI-AT 2024 brought together experts, engineers, researchers, and industry professionals to present the main applications of light-sheet microscopy to neuroscience and 3D cell culture. Participants also had the opportunity to put their newly acquired knowledge into practice through workshops at various sites and facilities of the Bordeaux node (IINS, BIC, VoxCell).
During these four days of intensive training, four major themes were addressed to cover the wide range of applications in neuroscience and 3D cell culture:
Large Sample Imaging – Clearing & Expansion
3D Cellular Models Culture & Imaging
Neuronal Network Imaging
Image Analysis
“The theoretical knowledge covered during the conferences was perfectly useful in the workshops, and I would even say the workshops allowed us to go even further!”
Aurelien Debonne, PRIMACEN platform
“This congress was very thorough, with presentations in the morning and workshops in the afternoon. The selected speakers covered the entire scope of microscopy with biological samples: the approach, sample preparation, and even microscope selection!”
Nadege Le Roy, TreeFrog Therapeutics
Finally, participants enjoyed convivial moments to share their experiences and discover new technologies offered by the industry professionals present during these days.
As for the next edition, see you in 2026!
The France-BioImaging Image Contest is back for its 6th edition!
This image contest is open to all within the imaging community: core facility staff and users, R&D labs teams and co-workers, students… Submit your best microscopy images for a chance to showcase your skills, research and creativity to the French bioimaging community and beyond, allowing people to see the visual appeal of the life sciences. Images from the contest will be featured on France-BioImaging communication tools, online and in print.
France-BioImaging and all the French community aims to develop and promote innovative imaging technologies and methods. But microscopy images can also take an artistic, creative look and make the invisible world beautiful.
We are all eager to see your work !
Prizes
1 to 3 images will be awarded depending on the quantity and quality of the entries submitted. France-BioImaging will cover the registration fees for one 2024 microscopy related event of the winners’ choice (FOM, ELMI, EMC, COMULIS conference, etc.).
Important: Only French or foreign participants affiliated to a French institution can enter the contest. Foreign participants non-affiliated to a French institution can submit images and will be featured in the gallery, but will not be evaluated as part of the contest.
Submission deadline: Friday, November 8th, 2024, 23h59 UTC+2.
This workshop will take place at the Institut Fresnel (FBI Marseille node) from November 12th to 14th , 2024.
Fluorescence polarization microscopy allows measurements of the orientation of molecules probed by various techniques. Through hands-on experiments, we will demonstrate their suitability for biological applications on a range of sample types, including cells and organisms. The Institut Fresnel is a pioneer in this field, developing unique microscopes to probe molecular orientation – an intrinsic property of molecules that is measurable with all types of microscopes but often remains inaccessible.
The workshop aims to offer (1) a theoretical basis for understanding how one can probe molecular orientation, (2) ample time for practical experiments, and (3) opportunities for formal and informal discussions on technical details and biological applications.
Free registration includes accommodation, transport, and meals (except for the evening of November 13th).
Candidates whose research projects align best with the workshop’s focus will be selected, with preference given to members of the France-BioImaging (FBI) infrastructure.
The France-BioImaging CLEM working group is pleased to announce the organization of the next CryoCLEM Workshop, to be held from October 14 to 15, in Bordeaux.
This workshop is the ideal event to be introduced to, and to gain knowledge of, the cryoCLEM technology, with the aim of applying it to your research project.
It will be composed of a theoretical part, presented by experts in this technology, and a practical part covering the entire cryoCLEM workflow (from sample preparation to correlative microscopy in cryogenic conditions).
Banner picture copyrights: images 1 & 3 R. Anger, image 4 P. Lapios, image 2 N. Pied
The first meeting of the FBI Mechanobiology WG will take place on the 2 and 3 of December 2024 at the Centre for Integrative Biology (https://cbi-toulouse.fr/fr/) in Toulouse..
The programme will include a seminar by Matthieu Piel (Institut Curie/Institut Pierre Gilles de Gennes), presentations by participants and practical mechanobiology workshops (a choice of 3 workshops from a dozen: optical tweezers, bioprinting, microfluidics, AFM, Brillouin, finite element modelling, mechanical confinement, force measurements, etc.).
Registration is free, but places are limited and priority will be given to contributors whose abstracts have been selected.
The choice of workshops will be made at a later stage.
Preliminary Programme:
Monday, December 2nd
1pm: Welcome and seminar by Matthieu Piel
2-6pm: workshops
6pm: poster session and buffet
Tuesday, December3rd
9am-12pm: presentations by participants (and coffee break)
Thomas Dehoux: AFM and Brillouin light scattering for mechanobiology: beyond stiffness
Sylvain Landiech: Probing 3D tissue rheology with a high-throughput microfluidic aspiration pipette
Hervé Turlier: From microscopy images to mechanical models of tissues and back
Sylvie Coscoy: Study of the dynamics of cell-matrix interactions and mechanics in photopolymerized 3D fiber networks
Joseph d’Alessandro: Mechanical plasticity revealed by traction forces of migrating epithelial cell trains
Luisa Bruno: Lymph node mechanics and its impact on immune cells
Jean-Baptiste Manneville: Role of nuclear mechanics in glioblastoma aggressiveness
Nicolas Biais: A world of bacterial superheroes: an introduction to mechano-micro-biology
Olivier Rossier: Using single protein tracking and super-resolution microscopies to decipher the inner life of mechanosensitive subcellular structures.
Marcelina Cardoso Dos Santos: Quantum dot-based FRET nanosensors for talin membrane assembly and mechanosensing
12pm: buffet
1.30-4pm: workshops
Call for abstracts
Abstract submission open until 15/09
TYPES OF PRESENTATIONS
Oral presentations: If you are selected for an oral presentation, you will have 15 minutes, including discussion (12 minutes presentation + 3 minutes Q&A).
Posters: If you are selected to present a poster, you will present your work at the dedicated poster session on December 2nd, afternoon.
Selected contributors will be notified at the end of September.
Registration/Abstract submission form
This form is currently closed for submissions.
Advanced microscopy workshop in Bordeaux from November 4th to 7th, 2024.
This advanced training course aims to (1) present the theoretical foundations, (2) clarify and synthesize the various existing approaches to both sample and instrument preparation, and (3) provide an overview of solutions for handling and processing the data acquired. These objectives will be addressed through the prism of two important biological fields of application: Neuroscience and 3D Cell Cultures. Indeed, the versatility of light sheet methods means that questions in these two fields can be addressed at a wide range of scales, from the whole brain or organoid, to the study of the nervous system of small living organisms or brain slices, right down to the single molecule inside spheroids. To address these themes, we will draw on the expertise of the Bordeaux FBI site, whether in neuroscience or in the growth and imaging of 3D cell cultures.
The course will be structured around 4 main thematic tracks, addressing the issues of sample preparation and data analysis for given samples. Participants will have the choice of following one of these tracks, or navigating between them according to their interests. The tracks will be :
P1: Large sample imaging – Clearing & Expansion
P2: 3D cellular models Culture & Imaging
P3: Neuronal network imaging
P4: Image Analysis
The format of the course will include lectures and seminars in the morning, providing a theoretical grounding in the various areas covered (sample preparation, imaging, image processing) and presenting the latest developments in these fields, and practical workshop in the afternoon on the various sites of the Bordeaux node (IINS, BIC, VoxCell).
Ihssane Idrissi / Rémi Galland (Interdisciplinary Institute for Neurosciences, Bordeaux, France)
Vincent Studer (Interdisciplinary Institute for Neurosciences, Bordeaux France)
Gustavo de Medeiros (Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland)
Georges Debrégeas (Jean Perrin Laboratory, Paris France)
Thai Truong (University of Southern California, Los Angeles USA)
Angela Getz / Mathieu Ducros (Bordeaux Imaging Center, Bordeaux France)
Alexandra Fragola (Institut des Sciences Moléculaires d’Orsay, Orsay France)
Emmanuel Faure (Laboratory of Computer Science, Robotics and Microelectronics, Montpellier France)
Johannes Roos (Johannes Kepler University, Linz Austria)
Philippe Girard (IJM, Paris, France)
Carole Siret (CIML, Marseille, France)
Guillaume Maucort (BIC, Bordeaux France)
Workshops on
Whole brains imaging by Ultramicroscopy
3D imaging of neuronal expanded samples by AxL (3i) microscope
3D entire small animal imaging
3D Cellular models culture and imaging using the soSPIM technology
Micro-niche creation for 3D cell culture and 3D imaging using the HS-ISM technique
Neurospheres culture and imaging using the MuViSPIM
Brain slices imaging using a Lattice Light Sheet Microscope
Single Cell electroporation for Brain slices labelling
Functional neuronal network imaging in ZebraFish
Orchestrating complex bioimage workflows using the Arkitekt solution
Napari for 3D data handling
How to segment a 3D dataset in just a few clicks?
Organizing committee
Coordinators: Mathieu Ducros & Rémi Galland
& FBI Work Group on « Multiscale Light-Sheet Microscopy »
Sponsors
On November 20th and 21th 2024, we have the pleasure to invite you to our Annual Meeting, to be hosted by our brand new FBI Alsace Node at the Bibliothèque Nationale et Universitaire (BNU) de Strasbourg (6 Pl. de la République, 67000 Strasbourg).
We will be happy to celebrate yet another year of achievements and developments in bioimaging with all the members of the community.
With a focus on “Live functional imaging: From chemical synthesis of the probes to instrumentation”, this edition aims to bring together chemists, microscope builders and biologists developping tools to probe life.
The scientific sessions will explore the development of new probes (organic, inorganic, nanoparticles, fluorescent proteins, hybrid materials), how to leverage their optical properties (spectra, quantum yield, lifetime, photostability, switching between dye states, etc…) and new instrumentations taking advantage of these new probes for life science.
France-BioImaging Mission Officers, Technology WGs, facilities or R&D teams are invited to present news, innovations or any achievements to the community with a poster. We strongly encourage you to submit an abstract for a poster presentation during your registration!
Registration is free but mandatory. Please note that the BNU capacity is limited and registrations will be accepted on a first come first served basis.
As part of the scientific activities that it wishes to carry out and in continuation of its 1st seminar (Dec. 2022), the FBI “Preclinical Microscopy” Working Group is organizing its 1st webinar on February 1 from 2:00 pm to 4:00 pm. The objective of these scientific meetings is to present research projects, technologies and regulatory frameworks related to light microscopy from the organ/organoid to the living animal.
Program
2:00 pm – Welcoming & Introduction
2:15 pm – “Visualization of the ticking of circadian clock cells in freely moving mice” by Xavier Bonnefont (Institute of Functional Genomics, Montpellier).
3:00 pm – “Regulatory aspects of the use of animals for scientific purposes” by Isabelle Bardou (Cyceron, Biomedical Imaging Platform, Caen).
On December 13th and 14th 2023, we have the pleasure to invite you to our Annual Meeting, to be hosted by our brand new FBI Toulouse Node at the Centre de Biologie Intégrative of the Université Toulouse 3 – Paul Sabatier.
We will be happy to celebrate yet another year of achievements and developments in bioimaging with all the members of the community.
This year, the Annual Meeting will focus on “Multiscale mechanobiology of cells and cell systems“, a topic specially selected for being one of Toulouse node’s expertise.
Mechanobiology aims to apply biophysical approaches to measure and perturb forces in complex physiological systems, and to develop in vitro systems including different types of organoids, enabling the controlled manipulation of cells and tissues and the measurement of mechanical forces to study cell mechanics and morphogenesis.
This “mechanobiology” theme is including (i) how cells generate and transmit forces, (ii) the impact of forces on cell/tissue dynamics, (iii) the impact of extrinsic forces on cells and tissues, and (iv) the development of new tools for manipulating and measuring forces in cells and tissues in a controlled, non-invasive way.
We invite the France-BioImaging Community to present their mechanobiology-related projects during the second day of the Annual Meeting around a dedicated session with selected talks. We strongly encourage you to submit an abstract for a talk or a poster presentation during your registration!
The winner of the best talk and the best poster presentation will win their registration fees for one 2024 microscopy related event of their choice!
Core facilities will also have an opportunity to present a poster.
En venant de Bordeaux, contourner Toulouse par l’Est (direction Montpellier) ; avant le péage prendre la sortie no 23 direction Rangueil ou la no 19 direction Ramonville, puis suivre Université Paul Sabatier.
En venant de Montpellier, après le péage, prendre la sortie no 19 direction Ramonville, puis suivre Université Paul Sabatier.
Une navette et le tramway relient l’aéroport et le centre ville de Toulouse (Transports en communs)
De là, il est possible de prendre le métro (lignes A ou B) pour rejoindre la station « Ramonville St Agne » (ligne B, direction Ramonville) : environ 1h de trajet
Le CBI est à 500 mètres à l’Ouest de la station et accessible à pieds ou en bus.
Il est également possible de prendre un taxi depuis l’aéroport : compter au moins 1/2h de trajet hors heures de pointe
En train
Depuis la Gare SNCF Matabiau:
Prendre le métro LIGNE A direction Basso Cambo, changer à la station “Jean Jaurès” et prendre la Ligne B pour rejoindre la station « Ramonville St Agne » : comptez environ 40 minutes depuis la gare.
Le CBI est à 500 mètres à l’Ouest de la station et accessible à pieds ou en bus.
Hôtels conseillés :
Nous vous conseillons de privilégier des hôtels en centre-ville.
Prise en charge des missions:
Se rapprocher de votre noeud FBI (fonds mission), sauf pour les intervenants qui seront directement contactés pour la prise en charge de leur missions.
France-BioImaging and all the French community aims to develop and promote innovative imaging technologies and methods. But microscopy images can also take an artistic, creative look and make the invisible world beautiful, allowing people to see the visual appeal of the life sciences.
We enjoyed the diversity of the images submitted with many different microscopy techniques, models and applications represented. A big thank you to all the participants!
The National Coordination Team and the Executive Board are proud to announce the winners of the FBI Image Contest 2023:
1st Place: Laurent LE, Lévêque-Fort Team, Institut des Sciences Moléculaires d’Orsay
“In the blink of an eye”
COS7 fixed cell. Alpha-tubulin labeled with DNA-PAINT and imaged with Atto 647N. Axial information is obtained by virtual-SAF measurement known as DONALD.
SMLM Fluorescence Microscopy with DNA-PAINT with DONALD detection
2nd Place:Gonzalo QUIROGA-ARTIGAS, Team Contrôle cytoplasmique de la stabilité du génome, Centre de recherche en Biologie Cellulaire de Montpellier
“Tardigrade embryos protected by mother’s molt”
Tardigrades commonly align the time of molting with egg laying. In this image we observe a tardigrade molt covering three developing embryos (DNA in white). The microscopy technology applied was confocal microscopy, and the research aimed to investigate the synchronization of embryo development in tardigrades.
Confocal microscopy
3rd Place:Hugues LELOUARD, Gorvel team, Centre d’Immunologie de Marseille Luminy
“Intestinal octopus”
Small intestine section from a LyzM-eGFP mouse containing one Peyer’s patch and stained for proliferative cells (Ki-67, yellow), Paneth cells (UEA-I, blue), epithelial cells (EpCAM, magenta), naive B cells (IgD, red), T cells (CD3, orange), helper T cells/macrophages (CD4, cyan), phagocytes (CD11c, turquoise), monocyte-derived phagocytes (GFP, green).
10-color spectral confocal microscopy
Congratulations to the winners!
Explore all the images submitted here:
As stated in the Terms & Conditions of the contest, foreign participants non-affiliated to a French institution are featured in the gallery, but were not evaluated as part of the contest.
The FBI Working Group Correlative Light-Electron Microscopy is organizing a workshop!
This workshop will take place at the Bordeaux Imaging Center (FBI Bordeaux node) from January 31st to February 2nd, 2024.
Correlative Light and Electron microscopy (CLEM) increases our capacity of biological investigation. By combining light microscopy and electron microscopy, this complementary approach takes advantages of both techniques. Light imaging provides valuable functional information thanks to its labeling power, whereas Electron microscopy excels at high resolution.
The Bordeaux Imaging Center has developed several workflows such as In-resin fluorescence and Array tomography. Both helps to determine 3D ultrastructure of a targeted area or a whole sample at different resolutions. In the framework of the FBI CLEM workshop, participants can choose one of these 2 different practicals, In-resin fluorescence and Array Tomography, in which a local specialist will show them the workflows.
Workshop 1: “In-Resin Fluorescence” – From HPF to tomography, by way of freeze-substitution and on-section fluorescence observation.
Workshop 2:“Array tomography” – From serial section to acquisition with confocal and SEM.
It is firstly intended to scientists with expertise in electron microscopy, who are expected to use the chosen technique.
2 workshops of 4 people each (you can apply only to one workshop)
A practical manual will be provided, covering every step.
You want to attend this workshop? Please fill the following form before December 22nd, 2023:
The candidates whose research projects suit the workshop the best will be selected. We will get in touch with you after the Christmas break to confirm your participation at this workshop.
This form is currently closed for submissions.
Nous vous invitons au premier webinaire sur l’Initiative Commune Afrique-France pour l’Imagerie Biologique qui se déroulera le 6 décembre 2023 à 11h00 CET!
En coordination avec l’African BioImaging Consortium et Africa Microscopy Initiative et dans le cadre du programme Horizon Europe, l’Initiative Commune Afrique-France pour l’Imagerie Biologique vise à étendre et renforcer les collaborations entre collègues africains et français intéressés par l’utilisation de microscopie avancées pour leurs propres projets de recherche. C’est dans cette optique que nous avons lancé deux appels à projet : l’un favorisant l’accès aux plateformes de bioimagerie de France-BioImaging, l’autre consistant à un programme de jumelage.
Ce webinaire sera notamment l’opportunité d’en apprendre plus sur les projets sélectionnés et sur les bénéfices de cette initiative pour les scientifiques africains et français.
Webinaire
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