Light-sheet based microscopy Symposium FBI Sponsored Events
October 4th, 2017, 9am-12pm
This Symposium is organized by the Réseau d’Imagerie Cellulaire Paris-Saclay.
Address: Hôpital Paul-Brousse, Bâtiment Inserm 15/16, Villejuif (see program for detailed map)
This is a free event.
Registration is free (mandatory) at : feuilldelum[at]gmail.com
Light sheet fluorescence microscopy (LSFM) is an emerging technology that combines optical sectioning with multiple-view imaging to observe tissues and living organisms with a remarkable resolution. Unlike conventional techniques of widefield and confocal fluorescence microscopy, the light sheet technique illuminates on the region surrounding the focal plane of the detection objective in a twin objective configuration.
Light sheet methods exhibit a reduced photobleaching and a lower phototoxicity. By rotating the specimen, the technique can image virtually any plane with multiple views obtained from different angles. LSFM is ideal for examining of both large (animals) and small (cells) specimens labeled with fluorescent proteins and other fluorophores.