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UTechS PBI – Pasteur

UTechS PBI – Pasteur

Director: Spencer Shorte

pbi.contact@pasteur.fr
Institut Pasteur, Rue du Docteur Roux, Paris, France

Photonic BioImaging is a Unit of Technology and Service (UTechS) providing optical imaging expertise in life sciences and especially their application in studies on infectious biology.

Our activities include service rendering, training, technology-driven research and technology development. They are highly multi-disciplined, and collaborative, with the mission goal focused on the use of quantitative imaging and analysis to understand the processes of cell/tissue-biology, and their usurpation by infection and disease. The R&D is founded upon the need to develop optical imaging methods that bring new understanding of host-pathogen interactions and in situ high-content imaging techniques and their application to infection, cell biology, cellular microbiology, and microbiology. We work on novel techniques extrapolating quantitative information on spatiotemporal dynamics in situ and we push the limits of existing approaches aiming to enhance their performance thereby broadening their experimental utility.

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PICT-IBiSA Curie

PICT-IBiSA Curie

Director: Olivier Renaud (PICT-LM) & Daniel Levy (PICT-EM)

Info.pict@curie.fr
Institut Curie, Rue d'Ulm, Paris, France

The Cell and Tissue Imaging Platform (PICT-IBiSA) of the Institut Curie brings together advanced microscopy technologies. Its main objective is to provide researchers in cell biology, development, structural, chemistry and biophysics with imaging approaches at different spatial and dynamic scales ranging from the molecule, to the cell, to the organism in healthy or pathological contexts.

The platform is organized around 3 poles: photonic microscopy, high-content screening, electron microscopy and cryo-electron microscopy. Photonic microscopy extends from dynamic imaging to high resolution. High throughput microscopy allows cellular screening of chemical and siRNA banks. Electron microscopy and cryo-microscopy provide the molecular structure and cellular ultrastructure of biological samples. The platform also offers expertise in data processing and analysis.

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Montpellier Ressources Imagerie

Montpellier Ressources Imagerie

Director: Patrick Lemaire

patrick.lemaire@crbm.cnrs.fr
Université Montpellier 1, Montpellier, France

Montpellier Ressources Imagerie (MRI) is a distributed imaging facility present on six sites in Montpellier (www.mri.cnrs.fr). MRI is labeled IBiSA and certified ISO9001-2008 LQRA. It has a staff of 30 engineers and is directed by P. Lemaire (CNRS). MRI manages numerous microscopes (36 photonic and 2 electron microscopes) and 14 analysis workstations, and especially microscopes for long term or short live experiments. MRI offers a complete set of state-of-the-art technologies, from single molecule to small organism imaging. The platform offers and develops 3D-SIM, SPIM, FCS/FCCS, CLEM and 2photons microscopies, and also develops a new service of High Content Screening, with a specific emphasis on gene expression analysis by smFISH techniques. MRI organizes regular training sessions with theoretical presentations and practical sessions about advanced light microscopy and image analysis. Once trained, a user can freely access microscopes on a pay-per-use basis. For the screening facility, the access is evaluated on a project-by-project basis.

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