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Bordeaux
146 Rue Léo Saignat, 33076 Bordeaux, France
16/12/2019 12:00 am
23/03/2020 12:00 am
10/04/2020 12:00 am
English
Description of the training activity
Rapid advances in live imaging of targeted cellular morphologies and functions underpin the emerging revolution in our understanding of synapses, circuits, and behaviour. In keeping with its long-established tradition, this Cajal course will assemble at its international faculty leading experts in developing and exploiting cutting-edge imaging techniques that have been propelling such advances. How to combine genetically encoded fluorescence labelling with behavioural designs, micro-circuit monitoring, or single-molecule tracking, how to avoid pitfalls of having false-positive observations and inherent noise, how to best analyse your multi-dimensional data will be, among others, the recurrent subjects of the Course.
Biological
Optics, Microscopy, Biological skills (Sample preparation, probes…), Image processing and data analysis
PhD students, Post-docs, Early career scientists (less than 8 years from the date of the PhD/doctorate), Core Facility Staff, Researchers
Laser scanning confocal microscope (LSCM / CLSM), Spinning disc confocal microscopy (SDCM), Deconvolution widefield microscopy, Multiphoton microscopy systems, Total internal reflection fluorescence microscopy (TIRF), Stimulated emission depletion microscopy (STED), Photo activated localization microscopy (PALM), Stochastic optical reconstruction microscopy (STORM), Reversible saturable optical fluorescence transitions (RESOLFT), Ground state depletion microscopy (GSD) / Ground state depletion, microscopy followed by individual molecule return (GSDIM), Fluorescence-lifetime imaging microscopy (FLIM), Fluorescence resonance energy transfer (FRET), Fluorescence recovery after photobleaching (FRAP)
Hard Skills - Metrology and Calibration From regular advanced LM up to High Res LM ( SIM, STED, STORM), Hard Skills - Evaluation and test on imaging software (per categories: coloc, 3D recons, registration, tracking...), Hard Skills - 3D reconstruction
Basic, Intermediate
Contact for registration/administrative aspects:
Antonella Caminiti