Thematic school 2021
All-optical interrogation of neuronal networks in vivo
Online, 25-28 May 2021
Thanks to the development of optogenetics, activity of neural networks can be recorded and modulated using optical methods. Thereby, these methods have become major tools for studying the neural mechanisms underlying perception, memory and behavior in animal models.
In this school, we will describe the different fluorescence microscopy techniques that have been developed and used to record neuronal activity in vivo, as well as the methods that enable modulating activity according to precise spatio-temporal patterns. We will first discuss the theoretical bases of these methods, and then we will present recent advances that have improved their speed, depth of penetration, field of view (2D and 3D), and applicability to the awake animal, both in head-fixed and unconstrained configurations. We will also give an overview of the palette of optogenetic tools available and the associated targeted labeling technologies. Finally, we will discuss the methods for analysis of raw functional signals, showing the wealth of information that these experiments can provide.
The school is designed for students and researchers in neurophysiology using optical methods and for physicists participating in their development.
Jerome Mertz (Boston Univ, USA)
Liangyi Chen (Peking Univ, CN)
Tommaso Fellin (IIT, IT)
Cathie Ventalon (ENS, FR)
Emiliano Ronzitti (Vision Inst., FR)
Isabelle Férézou (Neuro PSI, FR)
Jean-François Léger (ENS, FR)
Laurent Bourdieu (ENS, FR)
Sébastien Wolf (ENS, FR)
Stéphane Dieudonné (ENS, FR)
Vincent Villette (ENS, FR)
Volker Bormuth (UPMC, FR)