Training

The aim of this workshop encompasses most of the workflow steps from the 3D-sample preparation (organoids, spheroids, encapsulated 3D cultures), how to process them (histology, staining), how to mount them (for upright, inverted etc…), and finally how to image them with various microscopy techniques (from super resolution microscopies -liveSR or STED- to microscopies dedicated to thick samples (two-photon, ultramicroscope), from fast optical scanning spinning-disk to High Content Screening -HiTech and LowTech-).

Bench work is possible thanks to the TBM Core Facility, and the microscopes are provided by the BIC facility and the LP2N (home-made setup).

The number of attendees will depend on the rules that will prevail at this time.
Seats will be limited.
Registration is free but mandatory https://wkshpimageoids.sciencesconf.org/
Registration deadline: 6th Sept at noon
The organisation committee will then select participants and contact them shortly after registration closure.

There will be 4 seminars with the following keynote speakers:
• Xavier Trepat -link- (IBEC, Barcelone)
• Fanny Jaulin -link- (Institut Gustave Roussy, Paris)
• Laura Broutier -link- (CRCL, Lyon)
• Charlotte Rivière -link- (ILM, Lyon)

Each seminar will be preceded by a talk from our industry partners: Corning, StemCell, TreeFrog Therapeutics and Idylle.
They will be accessible freely on a dedicated streaming platform, upon ad-hoc registration which will be set soon.

We presume there will be restrictions due to the sanitary circumstances, therefore we strongly encourage you to anticipate about your own protection and sanitary status.

CNRS Workshop on Expansion Microscopy

Programme available here: https://sites.google.com/view/microscopie-par-expansion/programme

The event will take place from November 15 to 19, 2021 at the PFIC UMS AMMICa imaging Facility of Gustave Roussy.

Registrations are free but required.

The number of places being limited to 15 people, we will select the candidates according to their motivations.

The registration deadline is September 15, 2021.

Fluorescence microscopy techniques play an essential role in biology and medicine. A very rapidly developing field concerns nanoscale imaging (“nanoscopy”), which today ties together different fields of biology such as structural and cell biology. Similarly, “single molecule” imaging and spectroscopy techniques are in rapid development and play a central role in life science research. All fluorescence microscopy methods are based on labelling samples with appropriate markers: fluorescent proteins, organic fluorophores or nanoparticles. All these type of markers are characterized by very complex photophysical behaviors, leading to blinking, chromatic conversion, photoreactivity, etc… However, nanoscopy and single molecule spectroscopy or imaging, specifically rely on the ability to understand and control these properties.

The school we offer is motivated by the fact that more and more laboratories around the world are committed to implementing advanced microscopy techniques, especially super-resolution, with an increasing number of dedicated platforms. However, there is a lack of overall knowledge about the selection of fluorescent markers, their properties and mechanisms, and the type of artifacts they can create. The school’s objective, through interdisciplinary teaching between physics, chemistry and biology, is to overcome this gap by transferring knowledge to the participants’ laboratories. Our school also aims to make participants aware of new developments in the field in order to prepare them to become major players in the breakthroughs that may result from them.