Light-sheet microscopy - from theory to practical aspects
• Description the general principle of light-sheet microscopy
• Practical workshop on the three main instrumentations that have been developed depending of the sample scale to be imaged (1 to 100 µm; 100 µm to mm; mm to cm)
• Overview of the sample preparation techniques:
• Presentation of the challenges raised by the data management and analysis of LSFM data
Rapid advances in live imaging of targeted cellular morphologies and functions underpin the emerging revolution in our understanding of synapses, circuits, and behaviour. In keeping with its long-established tradition, this Cajal course will assemble at its international faculty leading experts in developing and exploiting cutting-edge imaging techniques that have been propelling such advances. How to combine genetically encoded fluorescence labelling with behavioural designs, micro-circuit monitoring, or single-molecule tracking, how to avoid pitfalls of having false-positive observations and inherent noise, how to best analyse your multi-dimensional data will be, among others, the recurrent subjects of the Course.