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Atelier de microscopie confocale 2024

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Duration
General Aim
  • Biological
  • Medical
  • Core Facility Staff Career
Specific topics covered
  • Optics
  • Microscopy
  • Biological skills (Sample preparation, probes…)
  • Animal handling
  • Image processing and data analysis
Target audience of the training
  • Msc students
  • PhD students
  • Post-docs
  • Early career scientists (less than 8 years from the date of the PhD/doctorate)
  • Core Facility Staff
  • Core Facility Head
  • Researchers
  • Medical Officers
Île de France Sud
Plateforme Imagerie-gif, Avenue de la Terrasse, Gif-sur-Yvette, France
20/09/2024 12:00 am
07/10/2024 9:00 am
11/10/2024 5:00 pm
1800€

Description of the training activity

OBJECTIFS
- Acquérir et renforcer les bases théoriques de la microscopie photonique
- Approfondir et maîtriser l'utilisation pratique d'un microscope plein champ et confocal
- Intégrer les principes et possibilités des nouvelles applications et développements en microscopie photonique
- Comprendre les complémentarités des techniques de la microscopie conventionnelle à la super-résolution

PUBLICS
Chercheurs, ingénieurs, techniciens
Prérequis : une expérience en microscopie photonique est essentielle.

PROGRAMME
Cours (40 % du temps)
- Rappels de microscopie conventionnelle, vidéomicroscopie
- Introduction à la microscopie confocale (physique de l'instrument, acquisition, numérisation, échantillonnage, multiples marquages)
- Microscope confocal rapide à disque de Nipkow (Spinning-Disk)
- Comprendre et utiliser les fluorochromes en microscopie confocale, protéines et sondes fluorescentes
- Préparation des échantillons biologiques vivants et fixés
- Traitement et utilisation d'images 2D et 3D appliqués à la microscopie
- Applications particulières en microscopie confocale, FLIM, FRET, etc.
- Résolution augmentée et super-résolution (PALM, STORM, STED, SIM)
- Séminaires d'application
Travaux pratiques (60 % du temps)
Un cycle de dix travaux pratiques sur vingt heures permettra de renforcer le lien entre théorie et applications, de reconnaître les éléments mis en œuvre, d'acquérir des images, de les traiter et de les analyser avec ImageJ notamment.
Une journée sera également dédiée à la découverte de techniques avancées telles que l'excitation biphotonique, la microscopie en feuillet de lumière, les F-techniques (FRAP, photoactivation) et la déconvolution.

Biological
Microscopy, Biological skills (Sample preparation, probes…)
Post-docs, Early career scientists (less than 8 years from the date of the PhD/doctorate), Core Facility Staff, Researchers
Laser scanning confocal microscope (LSCM / CLSM), Spinning disc confocal microscopy (SDCM), Stimulated emission depletion microscopy (STED), Photo activated localization microscopy (PALM), Stochastic optical reconstruction microscopy (STORM), Fluorescence-lifetime imaging microscopy (FLIM), Fluorescence resonance energy transfer (FRET), Fluorescence recovery after photobleaching (FRAP)
Hard Skills - Sample preparation, Hard Skills - Training on standard imaging methods (video microscopy, confocal and spinning disk imaging, multiphoton imaging, etc.)
Download
Intermediate

Contact for registration/administrative aspects:

Romain LE BARS
romain.lebars@i2bc.paris-saclay.fr
https://cnrsformation.cnrs.fr/atelier-microscopie-confocale?mc=Microscopie

Contact for registration/administrative aspects:

Romain LE BARS
romain.lebars@i2bc.paris-saclay.fr
https://cnrsformation.cnrs.fr/atelier-microscopie-confocale?mc=Microscopie

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