Order by
Filter training by
Duration
General Aim
Specific topics covered
Paris-Centre
BioImage Informatics
Bretagne-Loire
EMBL Heidelberg, Meyerhofstraße, Heidelberg, Germany
14/06/2021 12:00 am
06/09/2021 12:00 am
10/09/2021 12:00 am
125€
225€
Description of the training activity
Virtual course: Course Overview
Correlative Light and Electron Microscopy (CLEM) allows precise localisation of an event of interest within cells and tissues. During this course, we will focus on a specific method that has been developed at EMBL that enables the location of fluorescent proteins with high accuracy at the ultrastructural level. The course will cover both the theory and practicals of high precision CLEM, as well as opportunities to discuss their research with CLEM experts and receive practical and troubleshooting advice.
Audience
This course is intended for scientists with experience in EM who want to further develop their skills in high-accuracy correlated microscopy.
Modules/Resources
The course will combine lectures and remote hands-on practical work covering all steps in the work flow including cryogenic sample preparation, ultramicroscopy, image registration and electron tomography.
Learning Outcomes
Participants will gain hands-on experience with the CLEM methods and learn tips from the people who developed the methods.
Biological
Microscopy, Biological skills (Sample preparation, probes…), Image processing and data analysis
PhD students, Post-docs, Early career scientists (less than 8 years from the date of the PhD/doctorate), Core Facility Staff, Researchers
Correlative light electron microscopy (CLEM)
Hard Skills - Training on innovative technologies (CLEM, AFM/LM, SMLM, DLSM/SPIM...) and transfer to imaging facility, Hard Skills - Evaluation and test on imaging software (per categories: coloc, 3D recons, registration, tracking...), Hard Skills - Sample preparation
Intermediate, Advanced
Contact for registration/administrative aspects:
Lea Hohmann