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Histologie : de la préparation d’échantillons à la validation des marquages par analyse d’image

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General Aim
  • Biological
  • Medical
  • Core Facility Staff Career
Specific topics covered
  • Optics
  • Microscopy
  • Biological skills (Sample preparation, probes…)
  • Animal handling
  • Image processing and data analysis
Target audience of the training
  • Msc students
  • PhD students
  • Post-docs
  • Early career scientists (less than 8 years from the date of the PhD/doctorate)
  • Core Facility Staff
  • Core Facility Head
  • Researchers
  • Medical Officers
Bretagne-Loire
8 Quai Moncousu, 44000 Nantes, France
22/03/2021 12:00 am
25/03/2021 12:00 am
Français
1800€

Description of the training activity

La formation portera sur les préparations des échantillons pour la microscopie (de la prise en charge de l'échantillon jusqu'à la coupe sur lame) et les méthodes de vérification de la qualité des échantillons préparés en vue de leur quantification par analyse d'image. Les caractéristiques de préparation de différents types d'échantillons seront présentées (tissus durs - os -, biopsie, organe entier...) et mises en pratique.
Cours et tables rondes (40 %)
- Préparation des coupes :
. détails des étapes de préparation des coupes histologiques paraffine et congelées
. présentation des diverses colorations, des protocoles, de cas d'écoles et d'erreurs typiques à éviter
- Immuno-marquages :
. préparations en immuno-histochimie sur coupe (DAB - Red chromogène...)
. éléments de traitement du signal et d'analyse d'image pour validation de la bonne préparation des échantillons
. aspects quantitatifs
. immunofluorescence - multiplexing
- Introduction à la préparation des gros échantillons : spécificités liées aux marquages et à la préparation des gros échantillons pour observation en microscopie
Travaux pratiques (60 %)
- Préparation des échantillons histologiques (mise en œuvre des protocoles présentés)
- Visualisation au microscope des échantillons préparés
- Acquisition et analyse des images dans les meilleures conditions (logiciels gratuits QuPath et ImageJ)
Possibilité de faire les TP, à des fins pédagogiques, sur des échantillons apportés par les stagiaires.

EQUIPEMENTS
Microtomes ; cryostat ; automate de coloration ; serial block face ; scanner de lame (fond et fluorescence) ; microscope champ large et confocal ; ordinateurs avec QuPath et ImageJ
Voir le site internet de la plateforme MicroPICell pour une description détaillée des équipements

Biological
Biological skills (Sample preparation, probes…)
Post-docs, Early career scientists (less than 8 years from the date of the PhD/doctorate), Core Facility Staff, Researchers
Hard Skills - Sample preparation
Download
Intermediate

Contact for registration/administrative aspects:

https://cnrsformation.cnrs.fr/stage-21290-Histologie--de-la-preparation-dechantillons-a-la-validation-des-marquages-par-analyse-dimage.html?stage=21290&axe=138

Contact for registration/administrative aspects:

https://cnrsformation.cnrs.fr/stage-21290-Histologie--de-la-preparation-dechantillons-a-la-validation-des-marquages-par-analyse-dimage.html?stage=21290&axe=138

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