A National Research Infrastructure for Biological Imaging

CLEM & FAST: From Research to Startup


We are proud to present two videos created in collaboration with Picta Productions and the Paris Centre Node. Xavier Heiligenstein (Curie Institute) and Arnaud Gautier (ENS) present their research and their work, funded by France BioImaging in their inception.

This research has led to the creation of CryoCapCell, which develops and manufactures new products for sample preparation in the field of electron microscopy, such as the CryoCapsule and the HPM Light µ machine.

Relevant Publications:

Heiligenstein, Xavier, Perrine Paul-Gilloteaux, Martin Belle, Graça Raposo, and Jean Salamero. “EC-CLEM: Flexible Multidimensional Registration Software for Correlative Microscopies with Refined Accuracy Mapping.” Microscopy and Microanalysis 23, no. S1 (2017): 360-61. doi:10.1017/s1431927617002483.

Heiligenstein, Xavier, Martin Belle, Frederic Eyraud, Graça Raposo, Jean Salamero, and Jerome Heiligenstein. “The HPM Live μ–From Live Cell Imaging to High Pressure Freezing in Less than 2 Seconds for Correlative Microscopy Approaches.” Microscopy and Microanalysis 23, no. S1 (2017): 1276-277. doi:10.1017/s1431927617007048.

Heiligenstein, Xavier, Jérôme Heiligenstein, Cédric Delevoye, Ilse Hurbain, Sabine Bardin, Perrine Paul-Gilloteaux, Lucie Sengmanivong, Gilles Régnier, Jean Salamero, Claude Antony, and Graça Raposo. “The CryoCapsule: Simplifying Correlative Light to Electron Microscopy.” Traffic 15, no. 6 (2014): 700-16. doi:10.1111/tra.12164.

Research and development of the FAST technology is now undertaken through the startup Twinkle Bioscience. FAST offers new perspectives for cellular imaging, notably for high content screening or genome editing.

Relevant Publications:

Pimenta, Frederico M., Giovanni Chiappetta, Thomas Le Saux, Joëlle Vinh, Ludovic Jullien, and Arnaud Gautier. “Chromophore Renewal and Fluorogen-Binding Tags: A Match Made to Last.” Scientific Reports 7, no. 1 (2017). doi:10.1038/s41598-017-12400-9.

Li, Chenge, Alison Tebo, and Arnaud Gautier. “Fluorogenic Labeling Strategies for Biological Imaging.” International Journal of Molecular Sciences 18, no. 7 (2017): 1473. doi:10.3390/ijms18071473.

Jullien, Ludovic, and Arnaud Gautier. “Des sondes fluorescentes hybrides pour l’imagerie « à la demande  » des protéines cellulaires.” Médecine/sciences 33, no. 6–7 (2017): 576-78. doi:10.1051/medsci/20173306006.

Li, Chenge, Marie-Aude Plamont, Hanna L. Sladitschek, Vanessa Rodrigues, Isabelle Aujard, Pierre Neveu, Thomas Le Saux, Ludovic Jullien, and Arnaud Gautier. “Dynamic multicolor protein labeling in living cells.” Chem. Sci. 8, no. 8 (2017): 5598-605. doi:10.1039/c7sc01364g.

Plamont, Marie-Aude, Emmanuelle Billon-Denis, Sylvie Maurin, Carole Gauron, Frederico M. Pimenta, Christian G. Specht, Jian Shi, Jérôme Quérard, Buyan Pan, Julien Rossignol, Karine Moncoq, Nelly Morellet, Michel Volovitch, Ewen Lescop, Yong Chen, Antoine Triller, Sophie Vriz, Thomas Le Saux, Ludovic Jullien, and Arnaud Gautier. “Small fluorescence-activating and absorption-shifting tag for tunable protein imaging in vivo.” Proceedings of the National Academy of Sciences 113, no. 3 (2015): 497-502. doi:10.1073/pnas.1513094113.
Highlighted in ‘This week in PNAS’ in PNAS 113 (3), 465-467 (2016).


CLEMcorrelative light and electron microscopyFASTprotein taggingvideo

• November 14, 2017