CLEM & FAST: From Research to Start-up

CLEM & FAST: From Research to Start-up


The dissemination of emerging technologies to end-users is a key objective of FranceBioImaging. It is indeed essential that developers can obtain feedback from the end-users on their technologies. It is equally important that end-users can feed the thoughts and work of the developers. France BioImaging has thus invested in the dissemination of recently developed technologies in the Paris Centre node in the form of short videos.

The first two videos focus respectively on a fast-developing correlative imaging method that combines fluorescence microscopy and electron microscopy, and on a powerful reversible fluorescent protein labeling technology. These two technologies (as well as others currently developed in the Paris Centre node of FranceBioImaging) led to the creation of two start-ups (CryoCapCell and Twinkle Bioscience) thus illustrating another side of the dissemination action engaged by the actors of FranceBioImaging.

We are proud to present these videos created in collaboration with Picta Productions and the Paris Centre Node. Xavier Heiligenstein (Curie Institute) and Arnaud Gautier (ENS) present their research and their work, supported by France BioImaging in their inception.

This research has led to the creation of CryoCapCell, which develops and manufactures new products for sample preparation in the field of electron microscopy, such as the CryoCapsule and the HPM Light µ machine.

Relevant Publications:

Paul-Gilloteaux, Perrine, Xavier Heiligenstein, Martin Belle, Marie-Charlotte Domart, Banafshe Larijani, Lucy Collinson, Graça Raposo, and Jean Salamero. “EC-CLEM: flexible multidimensional registration software for correlative microscopies.” Nature Methods 14, no. 2 (2017): 102-03. doi:10.1038/nmeth.4170. (

Heiligenstein, Xavier, Martin Belle, Frederic Eyraud, Graça Raposo, Jean Salamero, and Jerome Heiligenstein. “The HPM Live μ–From Live Cell Imaging to High Pressure Freezing in Less than 2 Seconds for Correlative Microscopy Approaches.” Microscopy and Microanalysis 23, no. S1 (2017): 1276-277. doi:10.1017/s1431927617007048.

Heiligenstein, Xavier, Ilse Hurbain, Cédric Delevoye, Jean Salamero, Claude Antony, and Graca Raposo. “Step by Step Manipulation of the CryoCapsule with HPM High Pressure Freezers.” Methods in Cell Biology Correlative Light and Electron Microscopy II, 2014, 259-74. doi:10.1016/b978-0-12-801075-4.00012-4.

Research and development of the FAST technology is now undertaken through the startup Twinkle Bioscience. FAST offers new perspectives for cellular imaging, notably for high content screening or genome editing.

Relevant Publications:

Pimenta, Frederico M., Giovanni Chiappetta, Thomas Le Saux, Joëlle Vinh, Ludovic Jullien, and Arnaud Gautier. “Chromophore Renewal and Fluorogen-Binding Tags: A Match Made to Last.” Scientific Reports 7, no. 1 (2017). doi:10.1038/s41598-017-12400-9.

Li, Chenge, Alison Tebo, and Arnaud Gautier. “Fluorogenic Labeling Strategies for Biological Imaging.” International Journal of Molecular Sciences 18, no. 7 (2017): 1473. doi:10.3390/ijms18071473.

Jullien, Ludovic, and Arnaud Gautier. “Des sondes fluorescentes hybrides pour l’imagerie « à la demande  » des protéines cellulaires.” Médecine/sciences 33, no. 6–7 (2017): 576-78. doi:10.1051/medsci/20173306006.

Li, Chenge, Marie-Aude Plamont, Hanna L. Sladitschek, Vanessa Rodrigues, Isabelle Aujard, Pierre Neveu, Thomas Le Saux, Ludovic Jullien, and Arnaud Gautier. “Dynamic multicolor protein labeling in living cells.” Chem. Sci. 8, no. 8 (2017): 5598-605. doi:10.1039/c7sc01364g.

Plamont, Marie-Aude, Emmanuelle Billon-Denis, Sylvie Maurin, Carole Gauron, Frederico M. Pimenta, Christian G. Specht, Jian Shi, Jérôme Quérard, Buyan Pan, Julien Rossignol, Karine Moncoq, Nelly Morellet, Michel Volovitch, Ewen Lescop, Yong Chen, Antoine Triller, Sophie Vriz, Thomas Le Saux, Ludovic Jullien, and Arnaud Gautier. “Small fluorescence-activating and absorption-shifting tag for tunable protein imaging in vivo.” Proceedings of the National Academy of Sciences 113, no. 3 (2015): 497-502. doi:10.1073/pnas.1513094113.
Highlighted in ‘This week in PNAS’ in PNAS 113 (3), 465-467 (2016).

CLEMcorrelative light and electron microscopyFASTprotein taggingvideo