Application de la stratégie de double réaction chimique BLISS aux unités p-hydroxyphényle et guaïaicyle de la lignine sur coupe de racine de lin. Observation du double marquage (+ autofluorescence) par microscopie confocale et représentation par projection maximale d’intensité. Taille de l’image : 510 x 510 microns.
FBI Industry Committee Special Prize: Nathanaël Prunet – California Institute of Technology with “Arabidopsis Inflorescence”
This is a live Arabidopsis inflorescence with young flower buds developing at the periphery. Cell walls have been stained with propidium iodide (grey). Fluorescent reporters were used to monitor the expression of the APETALA3 (AP3, green) and SUPERMAN (SUP, red) genes. AP3 is required for the development of stamens (the male organs), while SUP establishes the boundary between the male and female part of the flower. This picture was acquired using live confocal imaging, which allows us to describe the expression of several genes in both space and time, in the same live biological samples, with a precise cellular resolution. It finally allows us to understand a question that has been elusive for 25 years: how the male/female boundary is established during the formation of the flower. My research aims at understanding how flower buds are patterned as they form.
Jonathan Daniel, Institut des Sciences Moléculaires
Laurence Dubreil, APEX-UMR703 PAnTher INRA Oniris
Pierre-Olivier Strale, Interdisciplinary Institute for Neuroscience
Clémence Simon, Unité de Glycobiologie Structurale et Fonctionnelle, UMR8576
Jérémie Teillon, INSERM U1034
Morgane Rabineau, Inserm
Eve Gazave & Nicolas Rabet, Institut Jacques Monod-CNRS
Nathanaël Prunet, Caltech, Meyerowitz lab
Françoise Geffroy, CEA-DRF-NeuroSpin-UNIRS, Midas Team
Valeria Davi, ImagoSeine – Institut Jacques Monod – CNRS
Anna Smirnova, University of Strasbourg – GMGM
Debora Olivier, Institut Pasteur
Orestis Faklaris, Institut Jacques Monod
Xavier Baudin, Institut Jacques Monod
Mathieu P. Dailly, CMAS
Lucie Sengmanivong, UMR 144, Institut Curie, Paris
Marie Irondelle, Institut Curie
Thank you also to the core facilities staff and heads for having forwarded the contest to their users and for providing them state of the art bioimaging.