Don’t forget to register before June 15th for MiFoBio autumn school, 5-12th November 2021 (Presqu’île de Giens)!
Pre-registration here: http://imabio-cnrs.fr/mifobio/pre-registration/…,
Important information for the registered participants: an email with the links to access the training videos was sent on February 15th, 2020. Please check your inbox and SPAM folder!
If you did not receive it, please send us an email to firstname.lastname@example.org
If you registered after February 14th, 2021, you will receive an email with the links within two days.
France BioImaging (FBI) is organizing a remote training on Light-Sheet Fluorescence Microscopy (LSFM), which enable 3D imaging of biological samples with unprecedented spatio-temporal resolutions and low perturbing effects.
LSFM methods actually cover a large variety of implementations which allow imaging a wide range of sample types, from single cell to whole organs or organisms both live and fixed. These new imaging capabilities are revolutionizing the way we visualize our samples and address biological questions. However, imaging with a light-sheet microscope raises many questions about the choice of the set-up depending on the sample to image, the sample preparation and mounting protocols or the data management (storage, visualization, quantification). Thus, it can be difficult to find its way through the numerous microscope implementations, protocols and tools that have been extensively developed over the last 20 years. We therefore decided to review all those questions in a remote training.
Our goal is to help people who want to jump into the world of 3D imaging and are seeking the best solution for their samples and biological questions. In that perspective, we will provide a comprehensive picture including all the possibilities and challenges regarding LSFM.
The training will be divided in 3 parts:
For the two first parts, videos will be available on a Youtube FBI channel. The participants will have 3 weeks, from the 15th of February to the 5th of March 2021, to watch those videos and will be invited to ask questions or comment.
FBI experts will then answer all questions during a live interactive video chat on the third week of the training (5th of March where participants will have the opportunity to directly interact with the experts.
Here are the three main questions concerning the imaging with a light-sheet microscope: (1) what LSFM type should I use for my experiment, (2) How do I prepare and mount my sample, and (3) how to visualize and analyze my data sets. The first part of this training will address these three questions through three theoretical courses:
In the second part of the training we will propose several videos on various systems available in the FBI laboratories and imaging platforms covering diverse types of LSFM design and applications.
Each video will feature a specific set-up and experts will present how to run an experiment on them focusing on three main aspects: (1) sample preparation and mounting methods, (2) image acquisition processes, and (3) visualization of the data-sets.
An online video session will conclude the training where FBI experts will answer all participants’ questions. You can ask questions either in advance in the comment box of the Youtube video, or during the Q&A session in a chat box. The Q&A session will be divided in sections, each related to a specific video.
In order to register to the Light-Sheet Fluorescence Microscopy remote training, please fill out the registration form available here.
Registration is free but mandatory in order to receive the links to the training videos.
Extended deadline: February 19th, 2021
We look forward to your participation !
The image depicts a spheroid of human stem cells (green) and its actin cytoskeleton (purple), produced by Philippe Cohen during its PhD at Treefrog. This nice picture serves as an illustration for an article covering the use of stem cells for regenerative medicine.
Acquisition was made by Philippe Cohen on a scanning confocal microscope and 3D rendering was done by Jérémie Teillon using Agave software.
Agave is a free 3D visualization software, using light path-trace light rendering.
The Bordeaux Imaging Center team offers training and support on 3D commercial softwares such as Imaris and Arivis as well as other freeware such as Agave. Don’t hesitate to contact them (email@example.com) if you are interested in 3D rendering and visualization of your microscopy data!
Article (in French):
Organized by the neuroscience and photonics departments and imaging facilities of Bordeaux and Quebec, Neurophotonics 2017 is the fifth edition of a successful conference series devoted to new frontiers in microscopy and neuroscience.
The meeting brings together leading developers and practitioners of advanced optical approaches to study brain function, from receptor dynamics at synapses to sensory processing in intact brains.
It will cover: