Due to the current sanitary measures and uncertainty about future COVID-19 developments, the LSFM workshop to be held in Bordeaux in December has been cancelled. The organizers are currently working on a virtual format of the workshop. More details to come soon.

This workshop will be held in Bordeaux from December 1st to December 4th 2020 and aims to give an overview of the Light Sheet Fluorescence Microscopy techniques and their application possibilities, taking advantage of the available technologies and expertise within the France BioImaging research infrastructure and the broad light-sheet microscopy community. This workshop is organized by the members of the FBI Multiscale Light-sheet Imaging Working Group.

About the workshop

  • The workshop will last 3 ½ days
  • The workshop is built on the basis of an alternation between theoretical and practical sessions. Both will be in English and provided by national expert trainers.
  • On day 2 a mini-symposium will be organized to present the last developments in different LSFM domains (instrumentation, sample preparation and data analysis) done by international experts. This mini-symposium will be open to a larger audience in order to promote LSFM approaches to the local research community. Registration is free but mandatory (this does not apply to workshop participants). Register now
  • The workshop will encompass various theoretical aspects of LSFM from sample preparation, to image acquisition and first post-processing steps (3D reconstruction, visualization).
  • The workshop will span various scales that can be imaged with LSFM based on at least 4 different LSFM set-ups
  • It will be included in the future “FBI training passport” and corresponds to a level III module (“à la carte”). Therefore, “trainees” must demonstrate knowledge of Level I (basic imaging) and Level II (optical principles) imaging.

Objectives:

  • Describe the general principle of light-sheet microscopy
  • Present the three main instrumentations that have been developed depending of the sample scale to be imaged (1 to 100 µm; 100 µm to mm; mm to cm)
  • Give an overview of the sample preparation techniques
  • Present the challenges raised by the data management and analysis of LSFM data
  • Present the future of light-sheet microscopy

Audience:

This workshop is ideal for researchers, engineers, technicians, PhD students, and post-docs from public institutes and private companies with a prior knowledge in fluorescence microscopy techniques, and willing to become familiar with advanced techniques to answer specific biological questions.

Number of participants is limited to 20.

Practical workshops:

The practical workshops main objectives are to:

  • Provide an overview by experts of the 3 main implementations of LSFM depending of the sample size to be imaged:
    • Ultramicroscope (LaVision Biotech) – BIC
    • Invi Lattice Pro (Luxendo)
    • Z1 (Zeiss) – to be confirmed
    • LLS – BIC;
    • soSPIM – IINS
  • Give an overview of existing solutions for 3D reconstruction, Multiview merging and 3D+t visualization.

Participation fee

Academia: 200€
Industry: 600€

Preliminary program:

The workshop will last 3 ½  days with theoretical courses the morning and practical workshops the afternoon on 4 different set-up spanning the different sample sizes that can be imaged with LSFM:

  • Zeiss – Z1 (to be confirmed)
  • Luxendo – Invi Lattice Pro
  • LaVision – Ultramicroscope II
  • Lattice Light-sheet microscope – BIC
  • soSPIM – IINS

3 theoretical sessions will encompass various domains of LSFM:

  • General principle
  • Sample preparation
  • Data management

A mini-symposium will be organized to highlight recent national and international developments in LSFM.

Confirmed speakers:

  • Willy Supatto – Laboratoire D’Optique et Biosciences, Palaiseau – France
  • Christopher Dunsby – Faculty of Medicine, Imperial College, London – UK
  • Laura Batti – Wyss Center, Genève – Switzerland
  • Nicolas Renier – Laboratory of Structural Plasticity, ICM, Paris – France
  • Martin Weigert – EPFL, Lausanne

On the last day two round tables will be proposed to discuss about two hot topics in light-sheet fluorescent microscopy:

  • Clearing protocols
  • Live imaging: challenges and solutions

Participants are invited to bring their own samples if possible to test during an optional practical workshop on the last day.

A diner in Bordeaux is planned on day 2.

Venue:

The workshop will be held on the premises of the Bordeaux Imaging Center and the Interdisciplinary Institute for Neuroscience, in the Centre Broca Nouvelle Aquitaine, in Bordeaux, France.

Accomodation

Hotel booking is left to the participant’s initiative.

Here is a list of other hotels located in the historic center of Bordeaux city (quartiers des “Grands hommes”, “Quinconces”, “Hôtel de ville” and “Mériadeck”). All are close to the tramway stops of line A (direction Le Haillan-Rostan / Pin Galant – Stops: Hôpital Pellegrin, Saint-Augustin, François Mitterand):

Hôtel de France **

Hôtel Gambetta **

Hôtel la Porte Dijeaux **

Hôtel des 4 Sœurs **

Hôtel de l’Opéra **

Hôtel Le Chantry **

Citadines Centre Mériadeck Bordeaux ***

Hôtel Ibis style Bordeaux Mériadeck ***

La Maison du Lierre ***

Hôtel Ibis Mériadeck ***

Best Western Bordeaux-Bayonne Etche Ona ***

Adagio Bordeaux Gambetta ****

Mama Shelter

Local Organisers

Dr. Mathieu Ducros, Bordeaux Imaging Center

Dr. Rémi Galland, Interdisciplinary Institute for Neuroscience

Questions about the LSFM workshop can be addressed directly to the local organisation team : lsfm_workshop@france-bioimaging.org

Poster

FBI_LSFM_Workshop-AfficheDownload

Sponsors

[:en]France BioImaging is organising an “BioImage Analysis OpenDesk” session on November the 29th from 9:00 to 12:30, in its different nodes and online.

What is an OpenDesk ?

During the event, users in search for answers to image processing-related questions come to a dedicated spot, within the Facility. Individual questions are processed by BioImage Analysts, on a “first come-first served basis”.

Will I really get my image processing questions answered ?

We will work on that ! Depending on the topic, local BioImage Analyst may choose from one of those three options:
1-The problem might be solved locally, quickly: you will leave the Facility with either a procedure/advices on how to analyse your data.
2-The problem might be solved locally, but requires additional time to be processed: your Facility staff will propose an appointment so that a proper solution is found.
3-The problem might not be solved locally: we will take benefit of the France BioImaging infrastructure, through its dedicated transversal node (FBI-IPDM). Your local Facility staff will introduce you to specialists in the field, using remote communication means.

I’m in ! Where and when is it taking place ?

November the 29th, from 9:00 to 12:30.
Bordeaux — Bordeaux Imaging Centre, Photonic Unit, 1st floor, Centre Broca Nouvelle-Aquitaine
Montpellier
Paris Centre
Marseille — IBDM ground Floor  Luminy
Paris Sud
Nantes — Room 2 , ground floor IRS UN 8 quai Moncousu

If you can not join physically, join on https://rendez-vous.renater.fr/ipdm

By the way, what is FBI-IPDM ?

The objective of the BioImage Informatics – Image Processing & Data Management transversal node is to create a general framework and a complete and integrated image analysis and IT (Information Technology) solution to address a number of challenges in biological imaging and microscopy, as well as setting up a high performance grid-computing infrastructure dedicated to massive computational and data storage demands. The FBI-IPDM node proposes different IT frameworks to deal with the data flow from the different imaging nodes. FBI-IPDM node is thus transverse, by definition.[:fr]France BioImaging is organizing a “BioImage Analysis OpenDesk” session on November the 29th from 9:00 to 12:30, in its different nodes and online.

What is an OpenDesk ?

During the event, users in search for answers to image processing-related questions come to a dedicated spot, within the Facility. Individual questions are processed by BioImage Analysts, on a “first come-first served basis”.

Will I really get my image processing questions answered ?

We will work on that! Depending on the topic, local BioImage Analyst may choose from one of those three options:
1-The problem might be solved locally, quickly: you will leave the Facility with either a procedure/ advice on how to analyze your data.
2-The problem might be solved locally, but requires additional time to be processed: your Facility staff will propose an appointment so that a proper solution is found.
3-The problem might not be solved locally: we will take benefit of the France BioImaging infrastructure, through its dedicated transversal node (FBI-IPDM). Your local Facility staff will introduce you to specialists in the field, using remote communication means.

I’m in ! Where and when is it taking place ?

November the 29th, from 9:00 to 12:30.
Bordeaux — Bordeaux Imaging Centre, Photonic Unit, 1st floor, Centre Broca Nouvelle-Aquitaine
Montpellier
Paris Centre
Marseille — IBDM ground Floor Luminy
Paris Sud
Nantes — Room 2, ground floor IRS UN 8 Quai Moncousu

If you can not join physically, join on https://rendez-vous.renater.fr/ipdm

By the way, what is FBI-IPDM ?

The objective of the BioImage Informatics – Image Processing & Data Management transversal node is to create a general framework and a complete and integrated image analysis and IT (Information Technology) solution to address a number of challenges in biological imaging and microscopy, as well as setting up a high performance grid-computing infrastructure dedicated to massive computational and data storage demands. The FBI-IPDM node proposes different IT frameworks to deal with the data flow from the different imaging nodes. FBI-IPDM node is thus transverse, by definition.[:]

Les 29 Mai au 31 Mai prochain, nous organisons à Bordeaux une formation en Super Résolution qui traitera de la microscopie STED et des techniques basées sur la détection des molécules uniques (STORM, PALM, U-PAINT..). Elle se déroule avec une alternance entre cours théoriques (de la base vers les nouvelles techniques en développement), analyse d’images et ateliers pratiques devant les machines commerciales ou développées maison. Cette formation pourra se faire en anglais.

Dear colleagues, dear FBI community,

The National Coordination and Industry Board are proud to announce that the winners of the FBI Image Contest 2017 are:

1. Marie Irondelle – PiCT Institut Curie with “Biology’s Little Venice”

La petite Venise de la biologie © Carine Rossé, Emilie Lagoutte & Marie Irondelle, Institut Curie


La petite Venise de la biologie © Carine Rossé, Emilie Lagoutte & Marie Irondelle – Institut Curie
Confocal microscopy

Transparisation par U DISCO d’une glande mammaire murine régénérée à partir d’un transplant d’organoides mammaire murin.

2. Orestis Faklaris – Institut Jacques Monod – ImagoSeine with “The Tree of Life”


The Tree of Life © Orestis Faklaris, Nicolas Chevalier – Institut Jacques Monod
Ultramicroscope – light sheet microscopy

3D z-stack projection of transparised chicken embryo stomach. Label betaIII-tubulin – Alexa488.

3. Clémence Simon – UMR 8576 CNRS/Université Lille 1 with “When Chemistry Transcends Lignin”


Quand la chimie transcende la lignine ! 1 © Clémence Simon, Unité de Glycobiologie Structurale et Fonctionnelle, UMR8576
Microscopie confocale, MIP, BLISS

Application de la stratégie de double réaction chimique BLISS aux unités p-hydroxyphényle et guaïaicyle de la lignine sur coupe de racine de lin. Observation du double marquage (+ autofluorescence) par microscopie confocale et représentation par projection maximale d’intensité. Taille de l’image : 510 x 510 microns.

FBI Industry Committee Special Prize: Nathanaël Prunet – California Institute of Technology with “Arabidopsis Inflorescence”


Arabidopsis inflorescence 1 © Nathanaël Prunet, Caltech, Meyerowitz lab
Confocal microscopy

This is a live Arabidopsis inflorescence with young flower buds developing at the periphery. Cell walls have been stained with propidium iodide (grey). Fluorescent reporters were used to monitor the expression of the APETALA3 (AP3, green) and SUPERMAN (SUP, red) genes. AP3 is required for the development of stamens (the male organs), while SUP establishes the boundary between the male and female part of the flower. This picture was acquired using live confocal imaging, which allows us to describe the expression of several genes in both space and time, in the same live biological samples, with a precise cellular resolution. It finally allows us to understand a question that has been elusive for 25 years: how the male/female boundary is established during the formation of the flower. My research aims at understanding how flower buds are patterned as they form.

Thank you to all the participants for their great contributions:

  • Dario Donnarumma, Laboratoire Charles Coulomb UMR 5221 CNRS-UM
  • Filippo Piccinini, IRST
  • Aude Nommick, IBDM – Marseille University
  • Sébastien Marais, Bordeaux Imaging Center
  • Marie Held, Biochemistry, University of Liverpool, Levy Lab
  • Patrice Mascalchi, Bordeaux Imaging Center and Frédéric Saltel, INSERM U-1053, University of Bordeaux
  • Corrado Viotti, Institut de Biologie Moléculaire des Plantes, CNRS, Strasbourg – P. Genschik Lab
  • Marcello Delfini & Mathieu Fallet, CIML CNRS-INSERM-AMU
  •  Jonathan Daniel, Institut des Sciences Moléculaires
  • Laurence Dubreil, APEX-UMR703 PAnTher INRA Oniris
  • Pierre-Olivier Strale, Interdisciplinary Institute for Neuroscience
  • Clémence Simon, Unité de Glycobiologie Structurale et Fonctionnelle, UMR8576
  • Jérémie Teillon, INSERM U1034
  • Morgane Rabineau, Inserm
  • Eve Gazave & Nicolas Rabet, Institut Jacques Monod-CNRS
  • Nathanaël Prunet, Caltech, Meyerowitz lab
  • Françoise Geffroy, CEA-DRF-NeuroSpin-UNIRS, Midas Team
  • Valeria Davi, ImagoSeine – Institut Jacques Monod – CNRS
  • Anna Smirnova, University of Strasbourg – GMGM
  • Debora Olivier, Institut Pasteur
  • Orestis Faklaris, Institut Jacques Monod
  • Xavier Baudin, Institut Jacques Monod
  • Mathieu P. Dailly, CMAS
  • Lucie Sengmanivong, UMR 144, Institut Curie, Paris
  • Marie Irondelle, Institut Curie

Thank you also to the core facilities staff and heads for having forwarded the contest to their users and for providing them state of the art bioimaging.

The National Coordination

Image Contest FBI 2017

The France BioImaging Image Contest is back for its 2nd edition!

This image contest is open to all within the imaging community: core facility staff and users, R&D labs teams and co-workers, students… Submit your best microscopy images for a chance to showcase your skills, research and creativity to the French bioimaging community and beyond, allowing people to see the visual appeal of the life sciences. Images from the contest will be featured on France BioImaging communication tools, online and in print.

France BioImaging and all the French community aims to develop and promote innovative imaging technologies and methods. But microscopy images can also take an artistic, creative look and make the invisible world beautiful.

The National Coordination is eager to see your work !

Prizes

1 to 3 images will be awarded depending on the quatity and quality of the entries submitted. Prize winners will get to choose one option, between the following:

  • Registration and travel costs (flight from France to Hungary) to NEUBIAS Symposium (31 January-2 February 2018, Szeged, Hungary)*
  • Registration fees for Focus on Microscopy 2018 (25-28 March 2018, Singapore)
  • Registration fees for ELMI 2018 (5-8 June 2018, Dublin, Ireland)

* Prize winners already accepted to the NEUBIAS Training Schools may request that France BioImaging cover their fees as a prize. However, keep in mind that may you not win a prize, the registration fee for the event will still be due.

 

Submission deadline: Sunday 26 November 2017, 23h59 UTC+2. THE CONTEST IS NOW CLOSED.

Click here to consult the terms and conditions of the contest. When you are ready, submit your entry by filling the form below. You can check out last year’s entries for inspiration. One participant can submit several entries.

 

 

 

We are happy to announce the 4th Mini-symposium on Bioimage Informatics, which will take place the 27th of June 2017 in Rennes. This event is organized by the GDR ImaBio and France Bioimaging.

The idea of this annual workshop on Bioimage Informatics is to bring together experts from the fields of image analysis and biology, to promote exchanges between the scientists in these fields and to contribute to the creation and maintenance of a French community in the field of Bioimage informatics, which is continuing in gaining importance in the field of Bioimaging. This year, the workshop is organized as a one-day satellite of the “Imaging the cell” conference, held in Rennes the 28th-30th of June 2017 (http://www.atoutcom.com/imagingthecell2017/).

Details & registration: http://gdr-miv.fr/en/events/bioimageinformatics2017/

Correlative Microscopy (CLEM) takes an increasing value in scientific research.

The second France BioImaging course on correlative microscopies is jointly organized by Institut Jacques Monod, Institut Pasteur and Institut Curie.

The first day (free access, registration required) will be composed of a conference series from national and international speakers about the latest CLEM technologies as well as by participants who will follow the practical session in the following 4 days.

Practical training will be organized in 2 sessions of 2 days for 3 groups between the 3 organizing institutes (12 positions available).

The 2 sessions will be chosen among the 3 available:
– EMPACT2 CLEM / Tokuyasu (Institut Jacques Monod)
– Live Cell to HPF, Quick Freeze Substitution/fluorescence on sections and eC-CLEM alignment (Institut Curie)
– Live Cell to FIB-SEM / Cryo-CLEM (Institut Pasteur)

Registration on the FBI website, dead-line 31 March 2017

First day: free entrance
Practical sessions: 250.00 euros in total, selection on application form.

FBI Grand

On Friday April 14 2017, we will have the pleasure to welcome you to our Annual Meeting, to be held at the Curie Institute (Paris, France). This event, open to all members of the bioimaging community, aims to provide a platform to discuss pivotal subject matters in our field. This year, the France BioImaging Annual meeting will focus on the topic of “Future challenges in BioImaging”.

This main topic will be divided into six “challenges”, as described below:

CHALLENGES


Challenge 1: “QUANTIFICATION OF THE MOLECULAR DYNAMICS AND COORDINATION IN CELLS AND SMALL ORGANISMS, INCLUDING AT THE NANOMETER SCALE”
This session will focus on emerging in super-resolution, single-molecule and fluctuation microscopies. The session will bring together scientists working on new technological developments, on new probes, and new acquisition schemes including three-dimensional and in depth imaging. Specifically, we will aim to cover the following topics:

  • Quantification of dynamics in Super-Resolution, single-molecule, and fluctuation based microscopies.
  • Use of adaptive optics for in depth imaging in tissues or small organisms
  • New technologies for 3D imaging, including single-molecule localization and STED.
  • New probes for super-resolution and fluctuations techniques.
  • Micro-scale dynamics and molecular interactions by fluctuation techniques.
The main purpose will be to discuss and brainstorm on the key methodological challenges in this field in the near future (5 years).


Challenge 2: “IMAGING ARCHITECTURES AND PROCESSES OF LIFE, FROM MOLECULAR COMPLEXES TO MULTI CELLULAR SYSTEMS”
Imaging Microscopy to explore biological systems in space and time and across scales, including the observation of development, growth and aging of organisms, is a major challenge. This session is dedicated to future challenges in microscopy imaging to integrate molecular architecture, spatial and temporal control of gene expression, sub cellular dynamics and cell behaviors in morphogenesis and organogenesis.
Keywords: Multimodal an multiscale imaging; developmental biology; in toto 3D+time imaging; super resolution in correlative approaches; CLEM.


Challenge 3: “NEW FRONTIERS FOR IMAGING, SENSING, AND CONTROLLING BIOMOLECULES”
Methods for remote control of cellular processes by means of a trigger (light, magnetic field,…) have seen explosive growth. Recently, efforts have led to the engineering of powerful approaches in which fusing biomolecules with triggerable modules enables to turn their function on and off in response to the trigger action. Associated with intrumental tools (e.g. optical methods of active illumination or excitation beam shaping), these approaches have yielded an outstanding alternative  to standard genetic or pharmacological methods with much improved spatiotemporal resolution to analyze cell signaling and other molecular regulation in cells and multicellular complexes. On the other hand, new  probes and methods allow for visualization and sensing of molecules in live cell and during the development of multicellular organisms. This challenge should reflect the latest progresses on how the integration of chemistry and biology will frame our ways to investigate Life Sciences.

Challenge 4: “UNCONVENTIONAL IMAGING”

The combination of  imaging techniques with spatially and temporally tailored beams and reconstruction algorithms provide new approaches to cell imaging. These approaches exploit the properties of electromagnetic fields, such as polarization, spectrum or wavefront diversity, and new measurement schemes. We will discuss the implementation of such unconventional imaging techniques and the specific challenges that they face in terms of sensing, collection, data processing, and interpretation.

Unconventional imaging techniques include imaging from polarization/phase/aperture diversity, imaging using ultrafast pulses or wavefront control, imaging through turbid media


Challenge 5: “BIOIMAGE INFORMATICS, IMAGE PROCESSING AND MICROSCOPY DATA MANAGEMENT”

This session will focus on emerging technologies in life science regarding microscopy, informatics & applied mathematics. The purpose is to bring together scientists working in the area of informatics and scientists working in life sciences who uses microscopy as a main tool in their research. Specifically, this session will cover some of the following themes:
– Visualization, manipulation and analysis of large multidimensional images
– Image correlation and fusion of multimodal and multiscale images
– Data mining and machine learning in biological studies from microscopy images
– Spatiotemporal Modeling and simulation of biological mechanisms
– Information Technologies for distributed computing, smart data storage and interactive image data bases.

Challenge 6: “NEW BIOLOGICAL MODELS AND APPROACHES: HOW WILL THEY FRAME THE NEXT CHALLENGES IN BIOIMAGING?”

In recent years, the Life Sciences have experienced an impressive number of methodological revolutions, and many new fields of investigation have opened up. This is reflected in a diversification of our models of studies (new model organisms, plant and marine; stem cells hiPCs, IPCs…), new approaches of genetic engineering (genome editing, CRISPR/Cas), new large-scale programs (Brain imaging initiatives…) and new biological concepts (epigenetics…).
These discoveries and research directly impact or will necessarily impact current or future biological imaging technologies. The purpose of this session will be to reflect on and discuss the challenges ahead. It will allow, through the multi-disciplinarity represented within FBI and consistent examples, to define the major issues that our community will have to confront in the next 5 to 10 years.

Of the six challenges, four were selected to be part of the final program : Challenges 1, 2, 3 & 5. Challenges 4 & 6 will be represented during the poster session.

SCHEDULE & PROGRAM

Oral Sessions Schedule

Poster Session Program

CALL FOR ABSTRACTS

The call for abstracts is now closed.

 

However, you can still find specifications for your poster in the following document:

Abstract Submission & Guidelines

REGISTRATION

Registration is now closed. Thank you to all who submitted abstracts.

FREQUENTLY ASKED QUESTIONS

Who can participate?

The meeting is open to all members of the bioimaging community (within and outside the France BioImaging community) who are eager to share their thoughts, ideas and research on the challenges described above.

How can I participate?

Anyone can participate by submitting an abstract for an oral presentation as part of the sessions, or for a poster presentation.

How can I submit an abstract?

Abstracts are to be submitted at the time of registration.
Abstract Submission Guidelines
Abstract Template

Can I choose the kind of presentation I want to give?

During the registration process, one can choose whether to give an oral or a poster presentation. However, due to the limited amount of slots available for oral presentations, submissions that have not been selected to be part of a session can still be presented during the poster session. Candidates also have the option to apply for an oral presentation only, or a poster presentation only.

How much does the meeting cost?

Registration is free, and lunch and coffee breaks will be provided on site. However, travel and accommodation expenses are to be covered by participants.

LOCATION

Institut Curie, 12 rue Lhomond, 75005 Paris, France

CNRSLogoCurie

The “Next generation training in biological imaging” Symposium at Focus On Microscopy 2017, 12th of April in Bordeaux

In the frame of the Euro BioImaging PPII project, France BioImaging and its partners in the international landscape will organize for the first time at the FOM conference a symposium focused on the crucial topic of innovative trainings in biological imaging. In the last years, the training offer in terms of latest development in biological imaging has become more and more diversified and specialized, to meet the need of high level expertise in core facilities and technical support for user and the broad scientific audience. The event will gather the main European actors in biological imaging training to present and discuss training strategies, innovative training models and training organization. All FOM participants will be welcome to attend this morning parallel session. Do not forget to register to the FOM meeting, held for the first time in France.

Symposium Program: Click here
FOM General Information & Registration: FOM website

Dear colleague,
Dear FBI community,

Following the decision of the Executive Board of June 30, 2016, the National Coordination is proud to announce that the winners of the FBI Image Contest 2016 are:

1. Sébastien Mailfert – Centre d’immunologie de Marseille Luminy – Aix Marseille Université with “Dalton”

Dalton © Hugues Lelouard, Mailfert Sébastien & Mathieu Fallet CIML CNRS-INSERM-AMU

Dalton © Hugues Lelouard, Mailfert Sébastien & Mathieu Fallet CIML CNRS-INSERM-AMU
Confocal microscopy
Revealing sub-population of immune cells on small intestine by 10 colors spectral imaging

AND with “Le Saint Pierre Méditerranéen”

© Noushin Mossadegh & Mailfert Sébastien - CIML, CNRS-INSERM-AMU

Le St Pierre Mediterraneen © Noushin Mossadegh & Mailfert Sébastien – CIML, CNRS-INSERM-AMU
A l’aise comme un poisson dans l’eau, les spermatozoïdes se reposent dans leur habitat, qui ressemble à un œil, avant leur grande migration. Coupe d’un testicule de nouveau-né de souris. Marquage en immunofluorescence des noyaux cellulaires (DAPI) représentés ici en cyan. L’actine représentée ici en jaune, révèle le «squelette de la cellule» (phalloïdine marquée avec le fluorochrome Alexa-647). L’image représente 256×256µm sur 4096×4096 pixels. La coupe est d’une épaisseur de 20µm. Image de microscopie confocale sur Leica SP5 ; laser 405nm et laser blanc à 633nm ; objectif 40X, O.N. 1.25, immersion à huile.

 

2. Michael Lang – Institut Jacques Monod – ImagoSeine with “Fly Monster”

Fly monster © Orestis Falklaris & Michael Lang – Institute Jacques Monod, CNRS UMR7592 - ImagoSeine

Fly monster © Orestis Faklaris & Michael Lang – Institut Jacques Monod, CNRS UMR7592 – ImagoSeine
Multifocal confocal microscopy (spinning disk, Spinning CSU W1)
Drosophila third instar larval head, nuclear-RFP, neronal-GFP and green autofluorescence, 25x magnification, scale bar is 100 μm.

Thank you to the participants for their great contribution:

  • Sébastien Mailfert – Centre d’immunologie de Marseille Luminy – Aix Marseille Université
  • Ariane Peyret – Laboratoire de Chimie des Polymères Organiques – Bordeaux Imaging Center
  • Melina Petrel – Bordeaux Imaging Center – Université Bordeaux Segalen
  • Patrice Mascalchi – Interdisciplinary Institut of Neuroscience – University of Bordeaux – Bordeaux Imaging Center
  • Michael Lang – Institut Jacques Monod – ImagoSeine
  • Olga Nagy – Institut Jacques Monod, Drosophila Evolution Group – ImagoSeine
  • Théophile Déjardin – Institut Jacques Monod – ImagoSeine
  • Liu Zeng Zhen – Institut Jacques Monod – ImagoSeine
  • Melina Heuze – Institut Jacques Monod – ImagoSeine
  • Orestis Faklaris – Institut Jacques Monod – ImagoSeine
  • David Pereira – Laboratoire Matière et Systèmes Complexes – ImagoSeine

Thank you also to the core facilities staff and heads for having forwarded the contest to their users and for providing them state of the art Bioimaging.

The next edition of our Image Contest will open early 2017. Get ready!

The National Coordination

Dear (es) colleagues,

Registration deadline May 28, 2016
Registration: http://gdr-miv.fr/mifobio2016/pre-inscription/
September 30 to October 7, 2016
Club Belambra Seignosse, Landes
See the Poster

As you know, our school MIFOBIO on biological and technological advances in live imaging will take place in Seignosse from 30 September to 7 October 2016 (http://gdr-miv.fr/mifobio2016). This school is organized by the CNRS GDR “microscopy and imaging of living” and RTmfm network with the support of INSERM, France BioImaging, ITMO AVIESAN and EMBRC infrastructure. This year, in addition to the developmental biology models already used in previous years, this year the EMBRC infrastructure (European Biological Resource Centre Marines) will provide some biological models marine (sea urchins, sea squirts, jellyfish, sponges, etc …) to different stages of development.

This school is not reserved for experts in microscopy, but also aims to help “beyond the borders” by bringing the skills and thus provide access to the tremendous potential of imaging and microscopy for biology. To this end, we propose this year a new “challenge bio”.

“challenge bio”

Biologists, particularly those who do not usually use microscopy or wishing to test new imaging modalities can propose a biological problem for which they need imaging. It is not necessary to have a priori on imaging methods’ (acquisition, analysis and modeling) to implement. These are the people involved in imaging (acquisition and analysis) that can recognize the problems posed an interest or potential for their technical and propose experiments that will be conducted during MiFoBio.

The objective is to take advantage of the combination in one place of exceptional technological platform and cutting-edge expertise in the various disciplines, to clear new issues and pave the way for collaboration beyond school.

We invite you to register at the school through the website (http://gdr-miv.fr/mifobio2016/pre-inscription/) and if you wish to propose a “bio challenge” in imaging (localization, quantification , dynamic, interaction, multi-scale, …). Due to the large number of requests for participation at this school, selection of participants will be made on the basis of this information (300 seats).

 

cordially

Laurent Héliot, Serge Monneret, Tristian Piolot,

for the organizing committee MiFoBio

Affiche Mifobio2016 GB Dear all,

The second call for workshops in MiFoBio2016 is on!

To date, we have almost 80 workshops proposed, with 45 pre-selected. We thank all those who have brought these proposals. When selecting, a particular attention will be paid to the educational aspect, the innovative nature of the proposal, the close association of a biological problem addressed to the technological approach.

Pre-selected workshops will be validated once obtained the certainty that required material will be available during the school.
 

For this second call, we would like to reinforce certain themes, however not exclusively :

    • All type of home-made microscopy systems
    • optogenetics approaches
    • Imaging of scattering media, heterogeneous, outliers, …. (adaptive optics)
    • Application of biological models on small organisms (insects, fish, … but excluding mammals) for developmental biology
    • neuro-biological imaging
    • Photomanipulations
    • Bio-sensors
    • nonlinear imaging and non-conventional imaging (phase imaging, ….)
    • Microscopy with structured illumination
    • Contribution of microfluidics for microscopy
    • Modelling and simulation of molecular dynamics and cellular organization.

 

Procedure for proposing a workshop:

    1. Application to attend the thematic MiFoBio2016 school: go to the website of GDR-MIV: http://gdr-miv.fr/mifobio2016/ to get your identifiers.
    2. Then submit your workshop proposal via the dedicated website: http://ateliers-mifobio.fr
    3. Login using your GDR-MIV identifiers.
    4. Go in the “Workshops / Submit a new workshop” and fill in all the form fields.

In the “abstract” (1000 characters max.) Thank you to define the objective(s) of the workshop you propose. A “description” field (10000 characters) is available for a more detailed explanation. This will be used for evaluation.

The whole site is in English. Your proposals must be registered on the website before 23 April.

 

What changes from previous editions:

    • You can create and edit your workshop proposals online.
    • Workshops can have only two trainers/animators. For this purpose a specific mechanism has been put in place:

      o The main animator creates the workshop file.
      o Then goes in the “Workshops / List my workshops” section.
      o In the “Code for co-animator” he “copy / paste” the code and sends it by mail to his co-animator.
      o The co-animator connects to the site (after having pre-registred to MiFoBio). Then he goes in the “Workshops / List my workshops” section and in “Enter a co-animator code”

The workshops can be repeated two or more times during the school (the number of repetition is likely to be modulated up or down).

The workshops will be selected by the organizing committee.

The workshop team is at your disposal for any further information and can be reached at the following address
mifobio-co-atelier@services.cnrs.fr

 
The workshop team: Fabrice Cordelières, Sandrine Lecart , Christine Terryn.