Biology, physics, applied mathematics and complex systems modelling

IdFsud gathers an important task force in biology, physics, applied mathematics and complex systems modelling. These disciplines synergize to develop innovative methodologies and tools in microscopy imaging and image analysis for applications in developmental biology, cell biology, plant biology, physiopathology and neurosciences. IdF Sud gathers three core partners with internationally acknowledged expertise: BioEmergences (CNRS-IBiSA), Imagerie-Gif and Polytechnique LOB (Lab for optics & biosciences, CNRS – INSERM).
The imaging facilities of IdFsud are developed and coordinated by: Nadine Peyrieras (INAF, CNRS, Gif-sur-Yvette), Sandrine Lecart (I2BC, CNRS, Gif sur Yvette), Emmanuel Beaurepaire (Ecole Polytechnique, CNRS, Palaiseau).
The activity of the Ile-de-France Sud node (IdFsud) consists in developing novel optical biological imaging methods, in giving access to a broad range of imaging approaches to a large community, and in providing adequate training in light microscopy and CLEM.

Technological Innovations

  • New non-linear contrast: probing lipid order with polarized THG. LOB. Phys Rev X (2013)
  • Multicolor 2P Imaging of Brainbow tissue LOB/Inst Vision/ENS. Neuron (2014)
  • 3D+time image analysis of the digital lineage trees with the BioEmergences workflow (Nature Comm. 2016)

Some of the Innovative systems operated

  • Multicolor and polarisation multi-photon microscopy
  • Horizontal 2-photon microscope
  • Light-sheet microscopy

Tech transfer

  • Biosensors & Applications
  • Industrial partnershipp for light sheet microscopy
  • 3D+time multiscale data reconstruction workflows
  • Correlative microscopy
Facility

Imagerie-Gif

↗ Website ↗ Service + Info

Facility: Imagerie-Gif

Head: Sandrine LECART

sandrine.lecart@i2BC.paris-saclay.fr
Plateforme Imagerie-gif, Avenue de la Terrasse, Gif-sur-Yvette, France

Services on this Facility

Service

Microscopy Technologies

Facility

Imaging and Cytometry Platform (PFIC) – Institut Gustave Roussy

↗ Website + Info

Facility: Imaging and Cytometry Platform (PFIC) – Institut Gustave Roussy

Director: Tudor MANOLIU

tudor.manoliu@gustaveroussy.fr
114 Rue Edouard Vaillant, Villejuif, France

Services on this Facility

Service

Microscopy Technologies

Facility

MORPHOSCOPE – Polytechnique bioimaging facility

↗ Website + Info

Facility: MORPHOSCOPE – Polytechnique bioimaging facility

Scientific Director: Emmanuel BEAUREPAIRE

emmanuel.beaurepaire@polytechnique.edu
École Polytechnique, Route de Saclay, Palaiseau, France

Services on this Facility

Service

Microscopy Technologies

Facility

Imaging Cytometry platform (ImCy) – Genethon

↗ Website ↗ Service + Info

Facility: Imaging Cytometry platform (ImCy) – Genethon

Scientific Director: Daniel STOCKHOLM

Daniel STOCKHOLM
Généthon, Rue de l'internationale, Évry-Courcouronnes, France

Services on this Facility

Service

Microscopy Technologies

R&D team

NanoBio Team – ISMO

↗ Website + Info

R&D team: NanoBio Team – ISMO

Head: Sandrine LEVEQUE-FORT

sandrine.leveque-fort@u-psud.fr
Institute of Molecular Sciences of Orsay, Rue André Rivière, Orsay, France
R&D team

OV cytology and imaging (OV-CI) team – IJBP

↗ Website + Info

R&D team: OV cytology and imaging (OV-CI) team – IJBP

Head of the facility: Bertrand DUBREUCQ

bertrand.dubreucq@inrae.fr
Inrae, Versailles, France
R&D team

Advanced microscopies and tissue physiology team – LOB

↗ Website + Info

R&D team: Advanced microscopies and tissue physiology team – LOB

Head: Emmanuel BEAUREPAIRE

emmanuel.beaurepaire@polytechnique.edu
École polytechnique, Rte de Saclay, 91120 Palaiseau, France
R&D team

Nanoimaging: cell dynamics and quantitative biology team – LOB

↗ Website + Info

R&D team: Nanoimaging: cell dynamics and quantitative biology team – LOB

Head: Cedric BOUZIGUES

cedric.bouzigues@polytechnique.edu
École Polytechnique, Route de Saclay, Palaiseau, France

Key publications

  • A workflow to process 3D+time microscopy images of developing organisms and reconstruct their cell lineage. Emmanuel Faure et al. Nature Communications volume7, Article number: 8674 (2016)
  • Efficient second-harmonic imaging of collagen in histological slides using Bessel beam excitation. Nelly Vuillemin, Pierre Mahou, Delphine Débarre, Thierry Gacoin, Pierre-Louis Tharaux, Marie-Claire Schanne-Klein, Willy Supatto & Emmanuel Beaurepaire. Scientific Reports volume 6, Article number: 29863 (2016)
  • Self-organization of the human embryo in the absence of maternal tissues
    Marta N. Shahbazi, Agnieszka Jedrusik, Sanna Vuoristo, Gaelle Recher, Anna Hupalowska, Virginia Bolton, Norah M. E. Fogarty, Alison Campbell, Liani G. Devito, Dusko Ilic, Yakoub Khalaf, Kathy K. Niakan, Simon Fishel & Magdalena Zernicka-Goetz. Nature Cell Biology volume 18, pages 700–708 (2016)