Being designed in response to imaging challenges, the Roboscope is the product of a collaboration between Marc Tramier’s team (FBI Bretagne-Loire node) with Julia Bonnet-Gélébart, research engineer, Jacques Pécréaux’s team of the Institut Génétique & Développement de Rennes (IGDR), and the Inscoper company, spin-off of the lab. This technology could become a great timesaver for fluorescence microscopy.

Allowing the automation of fluorescence microscope acquisitions, the Roboscope is an embedded technology based on a deep learning algorithm. To be precise, it is a predesigned event-driven acquisition (PEDA) based on a learning automatization of any cellular changes tracked by fluorescence. Catching rare and fast cellular events then becomes possible!

The use of the Roboscope would also save precious time of research, providing users with results without the need to stand by the microscope during acquisition. This technology goes beyond as they will be able to recover the data already classified and with only the specific points of illumination that they have previously triggered. 

A broad range of applications

The teams have almost finished to develop an entire algorithm monitoring the cell cycle progression in mitosis. These events specific to the cellular division correspond to major challenges in the control and treatment of cancer progression (Kops, 2005). As the cell cycle study is needed to understand several biological processes helping the development of targeted drugs, the technology aims to monitor efficiently and automatically simple cell models through their division cycle. 

And this is not its only benefit: this automatized fluorescence microscopy acquisition can be adapted in very diverse fields. From a cell cycle progression analysis to specific analysis, organelles, proteins and biological events can be tracked or activated within cells. A noteworthy advantage of the integrated device that – we hope – will be deployed widely in the future. 

Workflow of a Roboscope experiment. 1. The user annotate a bench of images with different class of interest to be detected. 2. The pre-trained Convolutional Neural Network is adjusted for the experiment by fine tuning and/or transfer learning. 3. The algorithm is transfered on embedded systems to perform real-time image analysis during microscopy acquisition. 4. The biological application with event-driven acquisition is defined and started by the user in order to start, interrupt and parametrize different acquisition sequence following real-time image analysis and event classification.

France-BioImaging, with its partner the GDR IMABIO, organizes the 4th edition of the FBI-AT: an advanced microscopy workshop to be held in Paris from November 21st to 25th, 2022.

The aim of this France-BioImaging-Advanced Training is to train microscopy users on the most advanced imaging techniques that will allow them to perform molecular studies at the cellular level as well as in thick samples. In particular, recent developments on fluorescent probes will be highlighted. The workshop will benefit from state-of-the-art equipment available on several of the Parisian Node Imaging facilities.

This year’s edition will have plenary lectures given by experts in the microscopy development field. These seminars will be advertised as a series and will be broadcasted for a large audience.  In addition, specific techniques will be introduced.

Hands-on practicals will train attendants on these techniques every afternoon in different sites in Paris including Institut Curie, Institut Pasteur, Institut Cochin, Institut Jacques Monod, Institut de Psychiatrie et Neurosciences de Paris and ENS Paris. Access to this part of training will be restricted to selected and registered trainees.

To guaranty access to set-ups and proper training, each practical session will host only 3-4 persons. The sessions will be run in parallel.

Apply now, attendance will be limited to 25 participants! 

FBI-AT is ideal for researchers with a basic training in microscopy willing to become familiar with advanced techniques to answer their specific biological questions, or to be exposed to new developments that will allow them to tackle new questions in their project. We will consider applications from early career researchers (PhD students, post-docs), technical staff members and more senior scientists.

Description
Programme
Registration
Venue
Gallery

AT A GLANCE

The workshop contains plenary lectures and specific training sessions. Plenary lectures will be on hybrid mode and largely open.

Invited Speakers

Emmanuel Beaurepaire

Giulia Bertolin

Joerg Bewersdorf

Peter Dedecker

Claire Deo

Marie Erard

Ricardo Henriques

Christophe Leterrier

Sandrine Lévêque-Fort

Gustavo Quintas

Gaelle Recher

Jean-Baptiste Sibarita

Lothar Schermelleh

Practicals on

  • Combining micro UV-irradiation and Single Particle Tracking in living cells
  • SMLM multi-color: from sample preparation to quantification
  • FRET-based molecular tension sensors and FLIM
  • Imaging of cellular ultrastructures with expansion microscopy
  • SIM, STED or STORM ? : from sample prep to 3D imaging
  • 3D STED : Comparing flat cells vs thick samples
  • Culturing and imaging multicolour 3D live brain organoïds
  • Combining fast live 3D imaging with Z resolution preservation
  • Light sources for optogenetics
  • Non-classical genetically modified fluorescent probes for biological imaging
  • Imaging biological structures in 3D using double helix-STORM and 3D-SIM

Organizers

Florence Niedergang, Lydia Danglot, Chloe Guedj, Mickael Lelek, Pierre Bourdoncle, Audrey Salles, Xavier Baudin, Nicolas Borghi, René-Marc Mege, David Geny, Ludovic Jullien

Poster

FBI-AT-2022-poster-V2Download

Sponsors

The France-BioImaging LSFM workgroup is pleased to announce an INSERM workshop on Light-Sheet Fluorescence Microscopy imaging technics. 

This workshop will be divided in two parts:

  • A first theoretical part in Bordeaux from the 16th to the 18th of May 2022 which will cover basic principles, applications and challenges of LSFM imaging through seminars,
  • A second practical part along June 2022 where you will have the possibility to choose the set-up on which you want to be trained amongst many systems available within the France-BioImaging community.

If you want to discover, learn and/or deepen your knowledge about this vast and powerful family of 3D imaging technics, this training is for you!


You will find all the information (program, registration details, venue and accommodations, …) about this event on the following poster and website (Atelier 268): https://evenium-site.com/site/atelier-de-l-inserm-268

REGISTRATION DEADLINE: March 4, 2022

Programme

Programme-268Download

Poster

Affiche-268Download

Following the new annual microscopy course “Principles and Applications of Fluorescence Microscopy” which is on offer for Masters and PhD students the teaching department and  the UTechS PBI (Imagopole) is organizing  a Workshop including both symposium (March 13th 2018) and  demonstration (March 12-16th). This workshop will be held on the campus and will run for until Friday, March 16th, 2018.

The symposium will be open to all researchers on the campus, but also to all guests and customers invited by the companies. This is a unique opportunity to bring together in one site the latest news of the greatest actors of fluorescence microscopy in Paris.

The event is free, but requires registration.

Please visit our website at https://www.pasteur.fr/en/FMWIII for the full program and list of speakers.

This event will include a series of seminars to explore the latest technological and scientific developments in fluorescence microscopy. The symposium, which will take place in the auditorium of the François Jacob building, represents a unique opportunity to hear from the leading experts in fluorescence microscopy about their latest research findings. It is open to all scientists on campus.
To coincide with this event, various companies will also be invited to display their state-of-the-art technological equipment with demonstrations in the Teaching Center. 12 companies have accepted the invitation to come and present their technology.
The event is free but prior registration is required: https://tinyurl.com/Microscopy-workshop