DNA-PAINT is a super-resolution imaging technique that relies on the transient binding of short fluorescent DNA “imager” strands to complementary “docking” strands attached to the target structure. Each binding event produces a localized burst of fluorescence that can be precisely detected and accumulated to reconstruct the image at nanometer resolution.

However, one major limitation remains: imager strands that are not bound continue to diffuse in the sample and emit fluorescence, creating background signal. This prevents researchers from using high imager concentrations and significantly slows down the acquisition process.

To overcome these limitations, a research team led by Yves Mely at the Laboratory of Bioimaging and Pathology (Strasbourg University) in collaboration with a team led by Alain Burger (Nice Institute of Chemistry) developed a new approach that incorporates a dark donor dye into the imager strand. A dark donor is a dye that remains almost non-fluorescent on its own but can transfer its energy to a nearby fluorescent acceptor when the two are brought together. In this system, the modified nucleobase X acts as the dark donor: it stays essentially dark in solution, but when the imager hybridizes with the docking strand labelled with ATTO 647N, X activates the acceptor’s fluorescence. As the signal appears only during true binding events, this fluorogenic behaviour markedly reduces background noise and enables the use of higher imager concentrations.

Schematic of the DRET-DNA PAINT concept. Oligonucleotides containing the dark donor X as a nucleoside substitute act as imager strands and transiently bind to the docking strands labeled with the acceptor dye ATTO 647N. This leads to DRET from X to ATTO 647N and thus to the turn-on of the acceptor emission

Single-molecule experiments confirm that the system maintains binding kinetics compatible with DNA-PAINT, and that fluorescence increases roughly 50-fold upon duplex formation. The method was then applied to fixed HeLa cells: microtubules were reconstructed in around 30 seconds, with a resolution of ~50 nm and a median localization precision of 18 nm. By comparison, classical DNA-PAINT required 30 minutes to reach a similar result.

When compared to FRET-PAINT, a variant of DNA-PAINT in which fluorescence is generated through energy transfer between a donor and an acceptor dye brought together during hybridization, the dark-donor strategy showed a clear advantage. FRET-PAINT can suffer from signal leakage, as the donor dye may emit light in the acceptor detection channel. In contrast, the dark-donor system produced far less leakage, leading to cleaner images while preserving a similar acquisition speed.

Composite of TIRF projection and super-resolution image reconstruction of microtubules in HeLa cells. a) DRET-PAINT with 100 nM S-Im imager strand and 30 seconds of imaging time. b) FRET-DNA PAINT with 100 nM S-Im imager strand and 30 seconds of imaging time c) DNA-PAINT with 1 nM of imager stand and 30 min of acquisition time. d) DNA-PAINT with 100 nM S-Im imager strand and 30 seconds of imaging time. Scale bar is 5 µm.

The main limitation of this first-generation system lies in the photobleaching of ATTO 647N, which shortens the usable imaging time. The authors suggest possible improvements, including the use of more photostable acceptor dyes or the development of new donor–acceptor pairs with enhanced brightness to support longer and higher-resolution acquisitions.

Overall, this work provides the first proof of concept that dark-donor DNA-PAINT can deliver fast, low-background super-resolution imaging and could become a valuable addition to the growing set of DNA-based nanoscopy tools.

F-BIAS is a professional network that brings together bioimage analysts across France. Hosted within France-BioImaging core facilities, its mission is to support researchers with high-quality expertise in image processing and analysis. Created in 2021, the network provides analysts with a strong community where they can share technical and methodological knowledge, and collaborate on innovative solutions.

F-BIAS also offers a monthly Open Desk in bioimage analysis: short sessions with imaging experts where you can ask any question related to image processing challenges you encounter in your research projects. If you need guidance in bioimage analysis, this is the perfect place to start!

No Open Desk available, or need more time to address your issue? F-BIAS also provides collaborative project support for more complex requests that require customized tools and a significant time commitment from analysts.

Join the network, discuss the challenges you face with your microscopy data, and let our experts help you find the best solutions!

Last month, several members of France-BioImaging took part in the opportunities offered by Euro-BioImaging’s EVOLVE project, enabling valuable exchanges and inspiring experiences.

Through the Job Shadowing initiative, Guillaume Gay, Data Engineer for the FBI.data mission and Research Software Engineer at LIRMM (Montpellier), welcomed Kenneth Ho, Big Data Systems Engineer at the Francis Crick Institute (London).

They discussed their shared challenges and solutions in microscopy data management, and this week-long visit gave them the time to delve deeply into several technical topics.

Caroline Thiriet, Deputy Administrative Director for International Relations and Industry at France-BioImaging, hosted Virginia Pierini, Service Manager at the EMBL Imaging Centre. Their discussions focused on how France-BioImaging coordinates its distributed national infrastructure, which brings together 10 Nodes and more than 30 imaging facilities across France.

©Euro-BioImaging – Fabrice Cordelières and Eva Svecova interview

Finally, Fabrice Cordelière, Head of Training for France-BioImaging and Bio-Image Analyst at the Bordeaux Imaging Center, took part in the Train-the-Trainer event, where he mentored Iva Švecová from the Light Microscopy Facility at the Institute of Experimental Medicine of the Czech Academy of Sciences in Prague. Eva benefited from Fabrice’s extensive experience in team management, user training, coding practices, and user-driven data backup workflows.

©Euro-BioImaging – Group photo of participants, trainers and members of the Euro-BioImaging Hub team

We are delighted to contribute to the EVOLVE programme, which fosters collaboration, peer learning, and mutual inspiration among Euro-BioImaging Nodes. These initiatives reinforce our shared commitment to open, coordinated, and high-impact scientific services for the life science community.

The microscopy platform of Gustave Roussy is organizing a webinar dedicated to Huygens Software on Tuesday, November 25th, at 14:00 (CET).

This event will introduce the theory of deconvolution and image restoration through three main parts:

  • Principles of Deconvolution: Dive into the theoretical foundations to better understand this key process in microscopy
  • Demonstration of Deconvolution and Image Restoration: Discover the essential features of deconvolution and image restoration, and learn how to automate these processes
  • Benefits, Limitations, and Quality Control: Identify the strengths and limitations of deconvolution while ensuring the quality and robustness of your results

You can share your own images for the demonstration and receive a direct feedback on the improvements in your restored images!

To send your files, please use the secure link here: https://svi.nl/upload

Registration is free but mandatory by simply emailing tudor.manoliu@gustaveroussy.fr

Join the webinar here: https://us02web.zoom.us/j/89769358929?pwd=aOQhDkLhJXkZnackSrYc6Aii6ZsyeU.1

Mitochondria, often described as the bioenergetic powerhouses of our cells, and more broadly of our entire organism, play a central role in metabolism. These organelles are involved in multiple metabolic processes (including carbohydrate and lipid degradation) and produce ATP (adenosine triphosphate), an essential molecule for a wide range of biochemical reactions.

Maintaining mitochondrial integrity is therefore crucial, as dysfunctions can lead to severe pathologies such as myopathies, neurodegenerative diseases or metabolic conditions like diabetes. Better understanding mitochondrial function and dysfunction is key to addressing these challenges. This understanding partly relies on the observation of mitochondria and the analysis of their morphology under different conditions.

Electron microscopy (EM) is the gold-standard technique for visualizing mitochondria, as it provides high-resolution imaging of cellular ultrastructure. However, segmentation and morphological analysis remain challenging due to the lack of contrast and color information in EM images. Existing pipelines to overcome these limitations are often time-consuming or too complex for users without experience in advanced deep-learning models.

To tackle this challenge, a research team from the Restore Institute in Toulouse, led by Mathieu Vigneau and Jean-Philippe Pradère, has developed EMito-Metrix, a computational tool designed for the automatic segmentation and analysis of mitochondria from 2D EM images.

The team created six species-specific models and one generalist model by training a segmentation algorithm with their own annotated EM images. Their results demonstrate that the tool enables highly specific detection of mitochondria according to their species of origin. With its user-friendly interface, EMito-Metrix allows users to easily visualize and analyze 26 mitochondrial metrics, presented through automatically generated graphs. In addition, EMito-Metrix includes a machine learning module that provides predictive analytical capabilities to assess how experimental factors, such as genetic mutations or drug treatments, may affect mitochondrial morphology and ultrastructure.

To validate their tool, the researchers analyzed mitochondria across the entire tree of life. More than 35 000 mitochondria were processed, with over 800 objects per species. The results obtained with EMito-Metrix are compelling, enabling precise segmentation of mitochondria by species and efficient quantitative analysis of their metrics.

The AI algorithm is capable of accurately detecting mitochondria (in colour) from tissues from different species imaged in ME (left). For each segmented mitochondrion, the tool extracts 26 morphology and ultrastructure metrics that can be displayed using graphs. The radar plot (centre) illustrates striking differences in metrics between vertebrates and invertebrates. Based on these metrics, the neural network is able to predict the class to which each mitochondrion belongs with 94% accuracy (right).

In conclusion, EMito-Metrix supports mitochondrial research by simplifying morphological analysis, saving researchers valuable time and reducing the risk of bias.

Access to EMito-Metrix solution here: https://www.emitometrix.org/

The RIC Paris-Saclay is pleased to announce the conference “AI & Image Analysis”, which will take place on Thursday, November 20, 2025, from 1:00 p.m. to 4:30 p.m. at the Paris-Saclay Faculty of Medicine (Research Building).

Organized in collaboration with the Interdisciplinary Object “BioProbe” (University Paris-Saclay), this half-day event is open to researchers, engineers, clinicians, faculty members, PhD students and students.

It will highlight recent advances in Artificial Intelligence and Deep Learning applied to cellular and tissue imaging, through presentations by both academic and industrial experts (University Paris-Saclay, University of Nantes, Institut Pasteur, École Polytechnique, Sartorius, and Nanolive).

The event will be recognized as doctoral training for the graduate schools BioSign, Cancerology (CMBS), and Therapeutic Innovation (ITFA) of UPSsaclay.

Invited speakers and titles

  • Perrine Paul-Gilloteaux (BioCore – University of Nantes) – AI in Correlative Microscopy: Unlocking the Potential of Multimodal Imaging.
  • Olivier Schwartz (Institut Pasteur) & Mathieu Fréchin (Nanolive SA) – Life in Motion: From AI-Enhanced Imaging to Cellular Process Simulations.
  • Anatole Chessel (LOB – École Polytechnique) – Understanding Complex Tissues from Microscopy Images.
  • Catherine Guettier (AP-HP / University Paris-Saclay) – Computational Pathology: From Code to Patient.

General information

Date: Thursday, November 20, 2025 – 1:00 p.m. to 4:30 p.m.
Location: Paris-Saclay Faculty of Medicine, Kremlin-Bicêtre
Free but mandatory registration: www.ric-paris-saclay.fr

Affiche_Conference_IA et AI_2025Download

Organization committee: Larbi Amazit, Régis Bobe, Laurent Combettes, Cécile Denis, Marie Erard, Evelyne Ferrary, Isabelle Garcin, Anne Guiochon-Mantel, Aude Jobart-Malfait, Valérie Nicolas, Oliver Nüsse.

As the 2025 edition of the France-BioImaging Image Contest is still running, we wanted to highlight our previous winners and their projects. Here is a quick throwback to our 2024 winners.

Before getting to the heart of the matter, we want to remind you that you still have time (before November 14th) to submit your best images and try to win your registration fees for one 2026 microscopy-related event! Please make sure you upload your images on the following link:

FBI Image Contest 2025

  • Improving DNA-PAINT speed with a fluorogenic DNA probe

    DNA-PAINT is a super-resolution imaging technique that relies on the transient binding of short fluorescent DNA “imager” strands to complementary “docking” strands attached to the target structure. Each binding event produces a localized burst of fluorescence that can be precisely detected and accumulated to reconstruct the image at nanometer resolution. However, one major limitation remains:…

Last year, we enjoyed the winning images submitted for their artistic take and their quality. Thanks to Vanessa WEICHSELBERGER , Dalia EL ARAWI and Frédéric FERCOQ for their beautiful images!

1st Place: Vanessa WEICHSELBERGER

Pierre-François LENNE team, Institut de Biologie du Développement de Marseille (IBDM)

“Rays of repetitive beauty”

Marine plant collected in Mediterranean Sea, stained for Actin using Phalloidin.

2-photon microscope

Vanessa WEICHSELBERGER is a shared post-doctoral researcher between the lab of Pierre-François LENNE at IBDM in Marseille and Vikas TRIVEDI at EMBL in Barcelona. She is a developmental biologist and interested in how cells coordinate morphogenetic processes with each other.

The image she submitted isn’t related to her research proejct. It is of a sample that they took from the Mediterranean Sea on a lab day out, it is part of an underwater plant. She believes it stands for their interest in shape and form beyond their own research projects. It represents their broad curiosity in biological structures.

They stained the sample for Actin and Nuclei and just admired its structure, pattern and periodicity.

“It is a great example of how amazing all kind of life is!“

By multiplying and arranging the image into a periodic wheel, the periodicity of the sample itself is even more highlighted.

2nd Place: Dalia EL ARAWI

Pierre-François LENNE team, Institut de Biologie du Développement de Marseille (IBDM)

“Gastrula Nebula”

Murine embryonic organoid illustrating cells collective migration on a laminin-coated surface. Phalloidin-labeled Actin filaments and Hoechst-stained Nuclei highlight detailed cellular architecture and a remarkably structured tissue organization.

Zeiss LSM 880 Confocal Microscopy

Dalia EL ARAWI is a post-doctoral researcher in biophysics, currently working at the IBDM in Marseille. Her research focuses on studying Gastruloids, 3D aggregates of mouse embryonic stem cells.

Gastruloids have emerged as a powerful in vitro model to study early embryonic development and tissue patterning. Within a few days, Gastruloids undergo symmetry breaking, axial elongation and germ layers specification, forming structures that closely mimic embryonic tissues both genetically and morphologically. Despite their ability to self-organize into complex architectures, 3D Gastruloids face developmental limitations over time, including tissue surface tension, apoptosis, and high phenotypic variability. To address these challenges, she used a hybrid 2.5D Gastruloids approach: first, generating 3D Gastruloids with proper differentiation and antero-posterior symmetry breaking, then transferring them onto a 2D extracellular matrix that mimics extraembryonic tissues, promoting more advanced morphogenesis.

This is illustrated in the submitted image, where a 2.5D Gastruloid spreads across a laminin-coated surface, revealing detailed cellular architecture and tissue organization. By applying different labeling strategies, she uses this approach to gain insights into vascular and cardiac structure formation. Her findings suggest that substrate-guided Gastruloid models may potentially recapitulate key aspects of cardiovascular development, offering new insights into tissue self-organization and vascularization.

For Dalia, being awarded second place in the FBI Image Contest was very rewarding. The recognition increased the visibility of her work, sparked discussions with colleagues inside and outside the lab, and motivated her to present her research more widely. It also gave her confidence to pursue future opportunities related to microscopy and imaging!

3rd Place: Frédéric FERCOQ

Molecules of Communication and Adaptation of Microorganisms team, Museum National d’Histoire Naturelle (MNHN)

“Explosion filarienne“

Internal architecture of Litomosoides sigmodontis, a parasitic nematode used as a model to better understand filarial infections. Under significant internal pressure to maintain its structure, this nematode experienced a cuticle rupture during handling, leading to the expulsion of some organs, including the ovary and intestine. The cytoskeleton appears in orange, and the DNA in cyan.

Confocal microscopy

Frédéric FERCOQ is a Maître de conférences in the UMR 7245 “Molecules of Communication and Adaptation of Microorganisms” at the MNHN in Paris. He works in the “Parasites and Free-Living Protists” team, where his research focuses on host–parasite–symbiont interactions during filarial infections. He studies how anti-parasite immune responses and bacterial endosymbionts such as Wolbachia influence parasite development, fertility, and tissue pathology. Microscopy is central to his approach, enabling him to visualize parasite structure, host tissue organization, and cellular interactions in detail.

The submitted image shows the internal anatomy of a female Litomosoides sigmodontis filarial worm after mechanical rupture. Due to the high internal pressure required to maintain its structure, the cuticle burst during manipulation, causing the expulsion of internal tissues, here the ovaries and intestine. Tubulin staining (orange) highlights microtubule-rich structures, while DNA is shown in cyan.

Winning the FBI Image Contest allowed Frédéric to attend the Microscience Microscopy Congress (MMC Series) in Manchester in July 2024, where he presented both a poster and an oral communication. The MMC is one of Europe’s leading interdisciplinary conferences dedicated to advances in microscopy across the life and physical sciences. It was a great opportunity for him to share their most recent publication (https://doi.org/10.1371/journal.ppat.1013301) and to present it in the context of their broader, microscopy-driven research on filarial development and host–parasite interactions. The event also allowed him to connect with researchers from diverse imaging backgrounds and explore new techniques relevant to parasitology.

Want to be the next winner of our FBI Image Contest? Apply through the following link before November 14th, 2025: https://france-bioimaging.org/fbi-special-events/fbi-image-contest-2025/

The brain: a network of neurons… and supporting cells

Our brain is made up of neurons…but not only neurons! Neurons are specialized cells that transmit information to other cells, whether nerve or non-nerve. Information travels as electrical impulses along the axon, a sort of “highway” that carries the nerve signal.

At the end of each axon are synapses, small communication zones composed of:

  • the axon terminal of the presynaptic neuron (which sends the signal),
  • the membrane of the postsynaptic neuron (which receives it),
  • and the synaptic cleft, the narrow gap where neurotransmitters are released to pass the message.

To keep neurons functioning properly, the brain also contains glial cells, which provide structural and metabolic support and regulate many physiological processes. Among them are astrocytes, star-shaped cells located close to synapses.

Since the 1990s and the discovery of the “tripartite synapse” concept, scientists have revealed the crucial role astrocytes play in information transmission.

However, astrocytes still hold some secrets, particularly concerning their finest extensions, called “leaflets”!

Cutting-edge imaging strategies for unprecedented results

To explore the structure and function of astrocytes in greater detail, the authors developed an innovative multimodal imaging workflow, combining two-photon and electron microscopy.

The electron microscopy experiments were led by several France-BioImaging units: CEA-Grenoble, Grenoble Institute of Neurosciences and the TIMC laboratory. The 2-photon imaging was performed at Lausanne University.

  • FIB-SEM microscopy to reveal the 3D ultrastructure of astrocytes and, in particular, their leaflets. This technique was combined with 3D immunolabeling to detect the presence of specific proteins:
  • IP₃R1, a marker of the endoplasmic reticulum involved in calcium signaling, 
  • Connexin-43, a protein found at gap junctions connecting different structures.
  • 2-photon calcium imaging to monitor real-time calcium variations in live leaflets in 3D, thereby assessing their role in cell-to-cell communication.

Architecture of the leaflets

Leaflets are extremely thin extensions (<250 nm), sometimes smaller than the wavelength of visible light, which explains why they long escaped direct observation.

They contain tiny segments of endoplasmic reticulum (i-ER) but no mitochondria, indicating a specialization for rapid and localized calcium signaling.

Several leaflets are interconnected via gap junctions, forming micro-networks within the astrocyte.

Figure 1. Leaflets are complex subdomains of the astrocyte interconnected by gap junctions. (B) Top: segmentation of a complete astrocyte fragment, including: bottom left: shafts with inner diameter >250 nm (gray); bottom right: leaflets, ≤250 nm (green). Bars: 3 μm.

Figure 3. i-ER: Minuscule organelles observed specifically in the leaflet domains of astrocytes. (C) Examples of i-ER saccules (magenta overlaid to EM images). Top: i-ERs surrounded by associated ribosomes (magenta arrowheads). Bottom: i-ERs contacting the astrocyte plasma membrane (yellow arrowheads). Bar: 500 nm.

Interaction with synapses

3D reconstructions revealed that the majority of synapses (around 90%) are shared by several leaflets of a single astrocyte.

Thus, one astrocyte can link together multiple synapses within the same neuronal network.

This finding challenges the “one synapse ↔ one astrocyte” model: in reality, astrocytes connect neurons to one another through their leaflets.

Figure 2. Leaflets are the astrocyte’s specialized subdomains that interact with synapses and preferentially embed multiple vs. individual synapses. (B) Arrangement of synaptic elements with leaflets and other astrocyte structures; 2D FIB-SEM images overlaid with segmentation map (leaflet: green; axonal bouton: yellow; spine: orange; synaptic cleft: red). Left: five synapses encompassed in a leaflet domain, contacted by leaflet units. Bar: 0.5 μm. Top right: synapse in direct contact with a shaft (S, gray); bar: 0.5 μm. Middle right: synapse in contact with both leaflets emerging from a shaft and the shaft itself; bar: 0.5 μm. Bottom right: synapse in contact with leaflets emerging from cell body (soma). Bar: 1 μm.

Calcium activity in leaflets

2-photon calcium imaging revealed very rapid, localized calcium micro-events, often triggered by neuronal activity.

These signals depend on IP₃R1 receptors in the i-ER and can propagate from one leaflet to another via gap junctions.

When they merge, these signals create broader calcium waves capable of synchronizing several neighboring synapses.

Figure 7. Leaflet domains host multiple synapses from independent circuits and display complex Ca2+ events that can integrate their ongoing activities in space and time. (B) Left: single-plane time-series capturing a representative multi-originated Ca2+ event in “Leaflets” (pseudocolor Ca2+ intensity scale, see “Two-photon 3D+t imaging data analysis” in STAR Methods). “Mitochondria” regions (azure pseudocolor) display an independent event. Right: oriented graph representation of the evolution of the event in “Leaflets,” visualizing spatial and time aspects, directionality, and intensity. (i), (ii), and (iii): timings of the images on the left. Arrowheads in (i) and (ii) identify distinct origination sites, and in (iii) their merging into a larger, long-lasting event. Bar: 1 μm. (C) Left: time-series as in (B), here capturing a Ca2+ event in “Leaflets” with three distinct originations (arrowheads). Two of them appear simultaneously in (i), the third, delayed, at another location in (ii), while in (iii) they all merge into a larger, long-lasting, high-intensity Ca2+ elevation. Noteworthy, the event expands around “Mitochondria” regions without touching them. Bar: 1 μm. Right: oriented graph representation of the Ca2+ event’s dynamics as in (B).

Conclusion

The results show that astrocytes are not mere support cells, but true biological computation units, where each leaflet acts as a functional “pixel” in a mosaic capable of interpreting and integrating multiple neuronal signals through the language of calcium.
This new astrocyte-oriented approach could pave the way for further studies exploring other brain regions to determine whether similar mechanisms exist elsewhere in the nervous system.

Read the scientific article here!

Benoit L, Hristovska I, Liaudet N, Jouneau PH, Fertin A, de Ceglia R, Litvin DG, Di Castro MA, Jevtic M, Zalachoras I, Kikuchi T, Telley L, Bergami M, Usson Y, Hisatsune C, Mikoshiba K, Pernet-Gallay K, Volterra A. Astrocytes functionally integrate multiple synapses via specialized leaflet domains. Cell. 2025 Sep 24:S0092-8674(25)01028-1. doi: 10.1016/j.cell.2025.08.036. Epub ahead of print. PMID: 40997814.

Last week, France-BioImaging participated in the Global BioImaging Exchange of Experience 2025. This year’s edition brought together scientists, facility managers, and infrastructure leaders from the international imaging community to explore “Imaging in 2035 – Sustaining Infrastructure Ecosystems & Advanced Technologies.”

Co-organized by BioImaging North America and Canada BioImaging, the event fostered rich exchanges among imaging infrastructure stakeholders on how to ensure the long-term sustainability of our global imaging ecosystem. France-BioImaging was represented by Caroline Thiriet and Jean Salamero who, as Mission Officer for Inter-Infrastructures Relationships at France-BioImaging and member of the Global BioImaging Working Group on Impact, moderated the session dedicated to “Micro-to-Macro: Measuring the Hidden Impacts of Imaging Scientists & Networks.” The session gathered a vibrant panel of experts — Susan Warner, Nick Souter, Johanna Bischof, Caron Jacobs, Leonel Malacrida, and Michelle Itano — who discussed how to communicate impact, reduce research’s carbon footprint, and recognize the essential contributions of imaging scientists worldwide.

The event also featured an inspiring keynote by Teng-Leong Chew (HHMI Janelia Research Campus) on “Microscopy Dissemination and Adoption Across the Globe.”

A big thank you to our global partners for an inspiring week of exchange and vision-building for the future of bioimaging!

We are pleased to announce the very first edition of our new series: FBI Connect! This webinar aims to share the latest techniques developed by France-BioImaging facilities and teams and how they can support the research. It will take place the Thursday, November 27 at 11:00 am.

For this first session, we will welcome Robert Quast (CBS, Montpellier), who will present a novel multicolor single-molecule FRET technique, unique in France and in Europe, which allows precise visualization of membrane protein dynamics.
He will illustrate its potential with an application to neuroreceptors, and more specifically metabotropic glutamate receptors, involved in synaptic transmission.
These receptors are of great therapeutic interest and a better understanding of their activation could contribute to the development of new treatments for depression, anxiety and neurodegenerative diseases such as Parkinson’s and Alzheimer’s.
This fast-growing technique can also be applied to other membrane proteins, making it relevant for other research fields such as cardiology or microbiology, offering new ways to observe pathogens.

To attend this first webinar, join us on Thursday, November 27 at 11:00 am.
Register now: https://u-bordeaux-fr.zoom.us/webinar/register/3417611245579/WN_JcBldv7lS9y-7pt_VsFa3A

This new edition, dedicated to the complete imaging workflow, from sample preparation to image analysis, will take place from November 5 to 7, 2025, in Versailles.

Program

The program includes numerous conferences and practical workshops on major topics:

  • Fluorescence lifetime imaging (FLIM/multi-color)
  • Live imaging – Biosensors
  • 2D and 3D electron imaging
  • Label-free imaging
  • Supervised learning, AI, and image analysis
  • Chemical imaging

Find the detailed program below:

Programme 14e Journee microscopistes INRAEDownload

This event is open to all! Whether you are a researcher, engineer, or technician, don’t miss this opportunity to network with other members of the community.

General information

When? November 5-7, 2025

Where? Centre INRAE Versaille-Saclay

The registration is free but mandatory – Deadline October 24

Find more information and registration link here: https://rmui-2025.journees.inrae.fr/