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Formation Histologie

de la préparation d’échantillons à la validation des marquages par analyse d’image

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General Aim
  • Biological
  • Medical
  • Core Facility Staff Career
Specific topics covered
  • Optics
  • Microscopy
  • Biological skills (Sample preparation, probes…)
  • Animal handling
  • Image processing and data analysis
Target audience of the training
  • Msc students
  • PhD students
  • Post-docs
  • Early career scientists (less than 8 years from the date of the PhD/doctorate)
  • Core Facility Staff
  • Core Facility Head
  • Researchers
  • Medical Officers
Bretagne-Loire
8 Quai Moncousu, Nantes, France
10/09/2024 5:00 pm
23/09/2024 9:30 am
27/09/2024 5:00 pm
French
2200€
2200€

Description of the training activity

La formation portera sur les préparations des échantillons pour la microscopie (de la prise en charge de l’échantillon jusqu’à la coupe sur lame) et les méthodes de vérification de la qualité des échantillons préparés en vue de leur quantification par analyse d’image. Les caractéristiques de préparation de différents types d’échantillons seront présentées (tissus durs - os, biopsie, organe entier...) et mises en pratique.
OBJECTIFS
- Acquérir des bases théoriques et pratiques sur la préparation des échantillons pour la microscopie photonique (lumière visible et fluorescence) et la quantification du signal
- Savoir préparer un échantillon en histologie (coloration - immuno-marquage)
- Connaître les différents types de préparation en fonction de la question biologique posée
- Savoir adapter la préparation des échantillons pour une analyse du signal solide et reproductible
- Savoir reconnaître les artefacts de préparation les plus courants et savoir vérifier la qualité de ses marquages à l’aide de logiciels d’analyse d’image

Biological, Medical, Core Facility Staff Career
Post-docs, Core Facility Staff, Researchers, Medical Officers
Laser scanning confocal microscope (LSCM / CLSM), Optical imaging, Phase contrast imaging, Sample preparation for histology, Virtual slides
Hard Skills - Emerging Imaging Methods in Medicine, including multiparametric Imaging, Hard Skills - Sample preparation, Hard Skills - Training on standard imaging methods (video microscopy, confocal and spinning disk imaging, multiphoton imaging, etc.)
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Basic, Intermediate

Contact for registration/administrative aspects:

CNRS Formation Entreprise
cfe.contact@cnrs.fr
https://micropicell.univ-nantes.fr/formation

Contact for registration/administrative aspects:

CNRS Formation Entreprise
cfe.contact@cnrs.fr
https://micropicell.univ-nantes.fr/formation

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