Description of the training activity
The workshop will last 3 ½ days and is built on the basis of an alternation between theoretical and practical sessions. Both will be in English and provided by national expert trainers.
On day 2 a mini-symposium will be organized to present the last developments in different LSFM domains (instrumentation, sample preparation and data analysis) done by international experts. This mini-symposium will be open to a larger audience in order to promote LSFM approaches to the local research community.
The workshop will encompass various theoretical aspects of LSFM from sample preparation, to image acquisition and first post-processing steps (3D reconstruction, visualization).
The workshop will span various scales that can be imaged with LSFM based on at least 4 different LSFM set-ups.
Learning outcomes of the training activity
• Get knowledge of light-sheet microscopy theory and working principles
• Have the opportunity to directly test the main commercial solutions available today that span the different sample sizes that can be imaged with a light-sheet fluorescence microscope
• Get the main principles of sample preparation for light-sheet microscopy (clearing approaches, samples mounting, ...)
• Have an overview of the solutions for LSFM data visualisations and analysis.