Order by
Filter training by
Duration
General Aim
Specific topics covered
Paris-Centre
25-28 Rue du Dr Roux, 75015 Paris, France
30/11/2019 12:00 am
02/03/2020 12:00 am
13/03/2020 12:00 am
English
Description of the training activity
The use of fluorescence microscopy (wide field, confocal, multiphoton, and now superresolution) in combination with genetically encoded fluorescence probes comprise a powerful set of scientific tools to study live cells. However, surprising little practical and theoretical training in such methods exists within standard curricula, particular at the early stage of training (Masters or Doctorate level). This course offers to cover basic optics principles necessary to understand the origin of microscope resolution and design. Participants will get hands-on experience implementing simple optical configurations to illustrate these fundamental principles. Subsequently, participants will perform experiments on state-of-the-art imaging equipment provided by microscope vendors.
Biological
Optics, Microscopy, Image processing and data analysis
Msc students, PhD students, Post-docs
Laser scanning confocal microscope (LSCM / CLSM), Spinning disc confocal microscopy (SDCM), Deconvolution widefield microscopy, Multiphoton microscopy systems, Total internal reflection fluorescence microscopy (TIRF), Stimulated emission depletion microscopy (STED), Photo activated localization microscopy (PALM), Stochastic optical reconstruction microscopy (STORM), Fluorescence recovery after photobleaching (FRAP), Selective plane illumination microscopy (SPIM), Digital scanned laser light-sheet fluorescence microscopy (DSLM), Phase contrast imaging
Hard Skills - Metrology and Calibration From regular advanced LM up to High Res LM ( SIM, STED, STORM), Hard Skills - Training on standard imaging methods (video microscopy, confocal and spinning disk imaging, multiphoton imaging, etc.)
Basic
Contact for registration/administrative aspects:
The organizers