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Super resolution in photonic microsocopy

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    Duration
    General Aim
    • Biological
    • Medical
    • Core Facility Staff Career
    Specific topics covered
    • Optics
    • Microscopy
    • Biological skills (Sample preparation, probes…)
    • Animal handling
    • Image processing and data analysis
    Target audience of the training
    • Msc students
    • PhD students
    • Post-docs
    • Early career scientists (less than 8 years from the date of the PhD/doctorate)
    • Core Facility Staff
    • Core Facility Head
    • Researchers
    • Medical Officers
    Bordeaux
    Centre Broca Nouvelle Aquitaine, 146 rue Léo Saignat, 33076 Bordeaux, France
    14/04/2020 12:00 am
    26/05/2020 9:00 am
    29/05/2020 6:00 pm
    English
    400€
    1000€

    Description of the training activity

    This training session will cover the basics of super resolution in photonic microscopy, principally: STED microscopy (STimulated Emission Depletion) and single molecule localization microscopy. We will also present alternative solutions for super resolution like SRRF (Super resolution Radial Fluctuations), Airy Scan, or Computer based pixel-reassignment like ISM (Image Scanning Microscopy), and solution based on light sheet illumination. The mornings will be dedicated to theoretical lectures and application seminars. The afternoons will allow the participants to see demonstrations of these different techniques in front of home-made or commercial systems. The fourth day will be a full hands-on day, as participants will be invited to bring their own samples and test them on the different systems available, like STED microscope, STORM, lattice light sheet, ISM, SRRF….

    Biological
    Microscopy
    PhD students, Post-docs, Early career scientists (less than 8 years from the date of the PhD/doctorate), Core Facility Staff, Researchers
    Stimulated emission depletion microscopy (STED), Stochastic optical reconstruction microscopy (STORM), Reversible saturable optical fluorescence transitions (RESOLFT), Selective plane illumination microscopy (SPIM)
    Hard Skills - Metrology and Calibration From regular advanced LM up to High Res LM ( SIM, STED, STORM)
    Download
    Basic

    Contact for registration/administrative aspects:

    Christel Poujol
    christel.poujol@u-bordeaux.fr
    http://www.bic.u-bordeaux.fr/training_2020/

    Location

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