France-BioImaging, with its partner the GDR IMABIO, organizes the 4th edition of the FBI-AT: an advanced microscopy workshop to be held in Paris from November 21st to 25th, 2022.

The aim of this France-BioImaging-Advanced Training is to train microscopy users on the most advanced imaging techniques that will allow them to perform molecular studies at the cellular level as well as in thick samples. In particular, recent developments on fluorescent probes will be highlighted. The workshop will benefit from state-of-the-art equipment available on several of the Parisian Node Imaging facilities.

This year’s edition will have plenary lectures given by experts in the microscopy development field. These seminars will be advertised as a series and will be broadcasted for a large audience.  In addition, specific techniques will be introduced.

Hands-on practicals will train attendants on these techniques every afternoon in different sites in Paris including Institut Curie, Institut Pasteur, Institut Cochin, Institut Jacques Monod, Institut de Psychiatrie et Neurosciences de Paris and ENS Paris. Access to this part of training will be restricted to selected and registered trainees.

To guaranty access to set-ups and proper training, each practical session will host only 3-4 persons. The sessions will be run in parallel.

Apply now, attendance will be limited to 25 participants! 

FBI-AT is ideal for researchers with a basic training in microscopy willing to become familiar with advanced techniques to answer their specific biological questions, or to be exposed to new developments that will allow them to tackle new questions in their project. We will consider applications from early career researchers (PhD students, post-docs), technical staff members and more senior scientists.

Description
Programme
Registration
Venue
Gallery

AT A GLANCE

The workshop contains plenary lectures and specific training sessions. Plenary lectures will be on hybrid mode and largely open.

Invited Speakers

Emmanuel Beaurepaire

Giulia Bertolin

Joerg Bewersdorf

Peter Dedecker

Claire Deo

Marie Erard

Ricardo Henriques

Christophe Leterrier

Sandrine Lévêque-Fort

Gustavo Quintas

Gaelle Recher

Jean-Baptiste Sibarita

Lothar Schermelleh

Practicals on

  • Combining micro UV-irradiation and Single Particle Tracking in living cells
  • SMLM multi-color: from sample preparation to quantification
  • FRET-based molecular tension sensors and FLIM
  • Imaging of cellular ultrastructures with expansion microscopy
  • SIM, STED or STORM ? : from sample prep to 3D imaging
  • 3D STED : Comparing flat cells vs thick samples
  • Culturing and imaging multicolour 3D live brain organoïds
  • Combining fast live 3D imaging with Z resolution preservation
  • Light sources for optogenetics
  • Non-classical genetically modified fluorescent probes for biological imaging
  • Imaging biological structures in 3D using double helix-STORM and 3D-SIM

Organizers

Florence Niedergang, Lydia Danglot, Chloe Guedj, Mickael Lelek, Pierre Bourdoncle, Audrey Salles, Xavier Baudin, Nicolas Borghi, René-Marc Mege, David Geny, Ludovic Jullien

Poster

FBI-AT-2022-poster-V2Download

Sponsors

Euro-BioImaging is organizing a third online User Forum on Tuesday, April 5, 2022 from 14:00-17:00 CEST. The topic is “At the Forefront of Neuroscience.”

This event will highlight the importance of cutting-edge imaging technologies in support of brain research and showcase the specific expertise available at our Nodes across Europe through case studies presented in tandem with the research community. 

In addition, keynote presentations from Hervé Boutin, University of Manchester, and Selma Ding, Max Planck Institute for Animal Behavior, will further reveal the potential of biological and biomedical imaging technologies to boost brain research. 


Register here: https://us02web.zoom.us/meeting/register/tZ0ucuytpj8iE92A-JjvPbvpNnmTF4Z7iRaC

More information: https://www.eurobioimaging.eu/news/join-us-for-the-third-euro-bioimaging-user-forum-at-the-forefront-of-neuroscience/

Programme

Euro-BioImaging-User-Forum-program_March-7Download

Deadline: April 15th, 2022

The three national infrastructures ProFi, France-BioImaging and FRISBI along with the GIS IBiSA are pleased to announce a call for a funded access to IBiSA-labelled facilities.

Our aim is to promote IBiSA facilities networking through interdisciplinary research projects.

Applications should request access to at least two different IBiSA facilities from two disciplines (structural biology, Biological imaging and proteomics). The call is open to any academic laboratory.

The amount of the financial support will be up to 5000 € per application to cover facility costs.

Applications should be submitted to: Call-IBISA-FBI-FRISBI-PROFI@i2bc.paris-saclay.fr
using the template document https://mycore.core-cloud.net/index.php/s/VNLU2jgfcP4o0NN


The deadline for this inter-infrastructure access call is 15 April 2022.

All submitted proposals will be peer-reviewed by independent experts and the final funding will be approved by a committee comprising 2 representatives from each infrastructure as well as representatives
from GIS IBISA. We advise the project PI to contact the chosen facilities in order to set-up the optimal experimental design.

Call description

Access-call-inter-infra-IBiSA-2022Download

The France-BioImaging LSFM workgroup is pleased to announce an INSERM workshop on Light-Sheet Fluorescence Microscopy imaging technics. 

This workshop will be divided in two parts:

  • A first theoretical part in Bordeaux from the 16th to the 18th of May 2022 which will cover basic principles, applications and challenges of LSFM imaging through seminars,
  • A second practical part along June 2022 where you will have the possibility to choose the set-up on which you want to be trained amongst many systems available within the France-BioImaging community.

If you want to discover, learn and/or deepen your knowledge about this vast and powerful family of 3D imaging technics, this training is for you!


You will find all the information (program, registration details, venue and accommodations, …) about this event on the following poster and website (Atelier 268): https://evenium-site.com/site/atelier-de-l-inserm-268

REGISTRATION DEADLINE: March 4, 2022

Programme

Programme-268Download

Poster

Affiche-268Download

Explore the beauty of the invisible world through the 2022 FBI digital calendar!

Enjoy the diversity of microscopy techniques, models and applications represented, one image at a time.

All 12 images used for this calendar were submitted to France-BioImaging image contest 2021. A big thank you again to all the participants!

You can download the A4 print version (one month per page) 2022 FBI digital calendar here:

France-BioImaging-digital-calendar-2022-vf-1Download

If you wish to use it as your computer desktop, you can download a PNG version of each month here:

January-22Download
February-22Download
March-22Download
April-22Download
May-22Download
June-22Download
July-22Download
AugustDownload
September-22Download
October-22Download
November-22Download
December-22Download

France BioImaging and all the French community aims to develop and promote innovative imaging technologies and methods. But microscopy images can also take an artistic, creative look and make the invisible world beautiful, allowing people to see the visual appeal of the life sciences. 

We enjoyed the diversity of the images submitted with many different microscopy techniques, models and applications represented. A big thank you to all the participants!

The National Coordination Team and the Executive Board are proud to announce the winners of the FBI Image Contest 2021:

  • 1st Place: Léna Meneux, Eye Team, Institut des Neurosciences de Montpellier

“The eye of the storm”

Sensory fibers of a mouse cornea imaged with a confocal microscope. The corneal nerves converge toward the centre forming a vortex. This particular transgenic mouse model allows stochastic expression of fluorescent proteins, unravelling the heterogeneity of the fiber origines inside the corneal epithelium.
Acknowledgements to Karine Loulier for the mouse model and Laetitia Hudececk for her help during the acquisition.

Confocal microscopy

  • 2nd Place: Eunice HoYee Chan, Muscle Dynamics Team, Developmental Biology Institute of Marseille (IBDM)
Myofibrils isolated from Drosophila indirect flight muscle labelled with titin (yellow) and actin (blue). Image captured from confocal microscope. We are studying the role of titin protein in muscle mechanics and organisation during development

“Sarcomeric bouquet”

Myofibrils isolated from Drosophila indirect flight muscle labelled with titin (yellow) and actin (blue). Image captured from confocal microscope. We are studying the role of titin protein in muscle mechanics and organisation during development.

Confocal LSM880
  • 3rd Place: Camille Boutin, Biology of multiciliated cells Team, Developmental Biology Institute of Marseille (IBDM) & Nicolas Brouilly, PICsL Imaging facility, Electron Microscopy department
Lamellar structure in a differentiating multiciliated cell observed by transmission electron microscopy with a Tecnai G2 200kV FEI.

“Clown”

Lamellar structure in a differentiating multiciliated cell observed by transmission electron microscopy with a Tecnai G2 200kV FEI.

Transmission Electron Microscopy, Tecnai G2 200kV FEI

Congratulations to the winners!

Explore all the images submitted here:

Fluorescence microscopy image illustrating skeletal muscle fibers (Desmin, green) in co-culture with motor neurons (SMI-32) forming active Neuromuscular Junction in a cell culture method using murin primary cells in vitro. Nuclei are stained by DAPI (blue).
Embryonic murine lacrimal gland (E17), with fluorescent staining showing Actin, Fibronectin, phospho-MLC and nuclei. The acquisition was performed using a confocal microscope Zeiss LSM 980, with a 25X air objective.
Confocal microscopie picture of a Drosophila larva's gut. Cyan: nuclei. Magenta: reporter for the activity of the protein GCN2. Yellow: the symbiotic bacterium Lactiplantibacillus plantarum. L. plantarum is a symbiont of Drosophila. We showed that it can communicates with its host by stimulating the activity of GCN2 in enterocytes. GCN2 may then trigger a physiological change of the gut that promotes larval growth despite malnutrition.
Representation of PALM technology on a rat hippocampal neuron. The low resolution image corresponds to the synaptic marker, Homer-GFP. Using PALM superresolution technology, it is possible to visualise NMDAR trafficking and clusters in high resolution. This approach can be used in the study of autoimmune neuropsychiatric diseases that disrupt both the organisation of NMDAR and their diffusion at the neuronal surface.
Neuronal spheroids of rat primary neurons are grown in hydrogel templates. The cells are electroporated with GFP and tdtomato. Then the spheroids are fixed and imaged with a confocal microscope. Cells in 3D structure mimic better functions and morphology properties of in vivo.
Myofibrils isolated from Drosophila indirect flight muscle labelled with titin (yellow) and actin (blue). Image captured from confocal microscope. We are studying the role of titin protein in muscle mechanics and organisation during development
The image shows the movements of Dopamine Receptors at the surface of hippocampal neurons and was acquired on a spinning disk. We took 1000 frames at 50ms exposition before reconstructing the trajectories of single receptors with PalmTracer. This allow us to understand, down to the nanometric scale, how the interaction between receptors modulate their surface diffusion.
Phages from vibro bacteria - Transmission electron microscopy
Patchwork of 4 maximum projections with different LUTs of a z-stack acquired in 2-photon microscopy. Neurons express the GCaMP6f calcium sensor following a viral infection of an organotypic mouse brain slice.
Negative staining artifact. Transmission electron microscopy.
In plants, the male gametes are represented by the microscopic round structures called the pollen. The anthers are the flower organs that produce and encase the developing pollen. This confocal microscope image is the cross-section of an anther of Arabidopsis thaliana. The developing pollen can be seen inside the anther. These are the haploid round cells containing protective pollen wall on their surface. The pollen are surrounded by diploid cell layers of the anther which nourish the young pollen supporting their development. This image was taken with the aims to understand how the growing pollen communicate with the surrounding nourishing tissues.
Aedes albopictus (Mosquito) leg imaged with a scanning electron microscope and digitally colored.
Deuterosomes producing centrioles during multiciliated cells differentiation.
Lagerstroemia sp. (Crepe Myrtle) blossom imaged with a stereomicroscope and digitally colored.
TEM observation of extracellular-vesicles from bacteria
PD1 membrane receptors super-resolution imaging in a PD1 stably expressing Jurkat cell. This acquisition has been performed with our soSPIM (single-objective Selective Plane Illumination Mocroscopy) system combined with an Adaptive Optics system (MicAO, Imagine Optique). The whole volume is composed of 35 Z-sections acquired every 500 nm, with each section consisting of 22,500 frames for a total acquisition time of 13h handled fully automatically. On each frame every molecule was precisely localized in 3D through an astigmatism-based process. The final cell exhibited more than 300,000 localizations and was 10 µm wide. To assess the spatial organization of clusters on the cell membrane, we used their centroids to compute a Voronoi diagram on the sphere approximating the cell shape.
Rat hippocampal neuron filled with a fluorescent marker and observed by dSTORM. The image shows details of dendritric spine morphology as well as surrounding axons in which we can observe the membrane-associated periodic skeleton.
Lamellar structure in a differentiating multiciliated cell observed by transmission electron microscopy with a Tecnai G2 200kV FEI.
Multiciliated cells at the surface of Xenopus embryo (Scanning Electron Microscopy)
A triple-negative cancer cell navigating its path through a dense 3D collagen matrix network. Collagen is labeled in green; Actin in magenta, and plasma membrane structure, caveolae, component in red. The image was acquired through a spinning disc confocal microscope for a 3D migration time-lapse experiment.
Ageratum bloom imaged with a scanning electron microscope and digitally colored.
Transmission electron microscopy observation of cells infected with Yersinia pseudotuberculosis in order to see their intra-vacuolar localization.
Two salivary glands from Drosophila simulans larva at L3 wandering stage. They have been stained with DAPI and rhodamine phalloidin. The upper gland is reconstructed in 3D on Imaris, nuclei and cells volumes are represented with a color scale from blue to red.

As stated in the Terms & Conditions of the contest, foreign participants non-affiliated to a French institution are featured in the gallery, but were not evaluated as part of the contest.

Euro-BioImaging first open call for user projects is open! If you have an idea for a biological or biomedical imaging project that you, your student or your close colleague could carry out in one of Euro-BioImaging Nodes, including France-BioImaging, now is the time to make this project come true with financial support from the Euro-BioImaging Access Fund.

How it works:

Submit your project proposal through the Euro-BioImaging web portal between October 20 and December 15, 2021, and indicate that you want to apply for the Euro-BioImaging Access Fund in order to be considered for a grant of up to 5.000 Euros to access the imaging services at a Euro-BioImaging Node. Projects will be evaluated by a committee of independent reviewers. Successful applicants will be notified by late January 2022 and successful projects should be started before July 2022.

What the funding covers:

The Euro-BioImaging Access Fund covers the user’s travel and accommodation costs as well as access and consumable costs at the imaging facilities that are part of Euro-BioImaging Nodes. For remote access projects shipment costs are also covered. Each successful applicant is eligible for up to 5.000 Euros of support.

Who is eligible:

All academic scientists, regardless of gender, nationality, home institution, career phase, or field of interest, are eligible to apply. We strongly encourage early career researchers to apply for this grant.

Projects should include transnational access to a Euro-BioImaging Node, i.e. the applicant’s home institution is in a different country than the Node where the project is to be performed. Due to the current sanitary situation, projects with non transnational access are elligible but transnational access will have priority.

All the external users/collaborators of France BioImaging facilities/labs are eligible:

  • any users from outside the institutional perimeter of France BioImaging nodes (i.e. from outside the following institutions: Aix-Marseille Université, Université de Montpellier, Université de Bordeaux, Université de Nantes, Université de Rennes 1, Université Paris-Saclay, Université de Paris, Université PSL -Paris Sciences & Lettres-, Généthon, Ecole Polytechnique, Institut Pasteur) who would like to use imaging technologies in one of FBI nodes: Paris Centre, Paris Ile-de-France-Sud, Marseille, Montpellier, Bordeaux, Bretagne-Loire. They can be French or international users – EU and non-EU
  • or users from a France BioImaging regional Node who want to access an equipment available in another FBI regional node.

Evaluation:

All applications will be evaluated for scientific excellence by a committee of independent reviewers. Selected projects will be assessed for technical feasibility and if needed receive technical advice from the Node providing the service.

How to apply:

Applicants are invited to visit our website to discover the range of technologies provided by Euro-BioImaging Nodes. Applicants will then follow the user access process described here: https://www.eurobioimaging.eu/about-us/how-to-access and indicate that they wish to apply for the Euro-BioImaging Access Fund in the application form.

The detailed procedure to apply to access one of France BioImaging facilities is available here: https://france-bioimaging.org/access/

Full details: https://www.eurobioimaging.eu/about-us/funding-user-access

Questions? Contact us!

SPAOM is a joint effort from Red Española de Microscopía Óptica Avanzada (REMOA) and the Portuguese Platform of Biomedical Imaging (PPBI), to organize an annual congress covering new applications in optical microscopy and image analysis.
This year’s programme offers 5 plenary sessions + 5 community workshops + numerous workshops and tech bites from commercial partners. 

REGISTRATION AND ATTENDANCE IS FREE FOR ALL PARTICIPANTS

The topics:

  • Open Microscopy, 3D/Live imaging, Super-resolution, Multiplexing/Multimodal, &  Networks/communities  (https://igc.events.idloom.com/spaom2021#section-speakers)
  • 5 community workshops: Frugal bioimaging, High-throughout/screening analysis; Machine Learning in Bioimage analysis; Phototoxicity; Remote Access…)
  • Several workshops by our sponsor partners: Agendo/Cirklo, Bruker, Zeiss | ILC, Izasa, Leica, Myltenyi Biotec, Olympus, Thermofisher  | 3i Intelligent Imaging Innovations, PhotonLines.

There will be also flash-talks, and a prize for the best communication from the submitted abstracts! The full list of speakers and scientific programme can be found here: https://igc.events.idloom.com/spaom2021

(the event will be online, through the Hopin platform, transmitted from Oeiras @IGCiencia)

Registration is here: https://igc.events.idloom.com/spaom2021/register

Euro-BioImaging is pleased to announce that the next special edition of the Virtual Pub, organized by Euro-BioImaging and the Euro-BioImaging Industry Board, will take place on December 10, 2021. In line with the season, this edition will focus on the theme of “COLD” in imaging.

They are inviting abstracts for 5 minute Flash talks about “COLD” in imaging. Be creative! Possible interpretations of “COLD” include:

  • Biology of the cold – from cold receptors and arctic organisms to brain reactions to cold and infections with common cold viruses
  • Cold-related metabolism including fat metabolism
  • Cold technologies – from cooling and freezing samples to low-temperature and cryo-imaging techniques
  • Keeping your sample or equipment cool – from reducing laser output and keeping high-power magnetic coils cool to incubating your sample at controlled low temperatures
  • Cold isotopes in molecular imaging

Please submit your abstract by November 22! https://www.surveymonkey.de/r/SpecialEditionCold

Prizes will be awarded to all speakers!

For more information: https://www.eurobioimaging.eu/news/submit-your-abstract-for-a-special-cold-edition-of-the-virtual-pub/

This image has an empty alt attribute; its file name is cold_Bioimaging_news.png

The France BioImaging Image Contest is back for its 3rd edition!

This image contest is open to all within the imaging community: core facility staff and users, R&D labs teams and co-workers, students… Submit your best microscopy images for a chance to showcase your skills, research and creativity to the French bioimaging community and beyond, allowing people to see the visual appeal of the life sciences. Images from the contest will be featured on France BioImaging communication tools, online and in print.

France BioImaging and all the French community aims to develop and promote innovative imaging technologies and methods. But microscopy images can also take an artistic, creative look and make the invisible world beautiful.

We are all eager to see your work !

Prizes

1 to 3 images will be awarded depending on the quantity and quality of the entries submitted. France BioImaging will cover the registration fees for one 2022 microscopy related event of the winners’ choice (FOM, ELMI, EMC, COMULIS conference, etc.).

Important: Only French or foreign participants affiliated to a French institution can enter the contest. Foreign participants non-affiliated to a French institution can submit images and will be featured in the gallery, but will not be evaluated as part of the contest.

Submission deadline: Friday, October 15th, 2021, 23h59 UTC+2. 

Click here to consult the terms and conditions of the contest. When you are ready, submit your entry by filling the form below. You can check out last editions’s entries for inspiration. One participant can submit several entries (up to 3).

This form is currently closed for submissions.

The first practical workshop « Imaging Organoids: from the bench to the microscope » will take place in Bordeaux, from Mon 27th Sept to Fri 1st Oct 2021.

The aim of this workshop encompasses most of the workflow steps from the 3D-sample preparation (organoids, spheroids, encapsulated 3D cultures), how to process them (histology, staining), how to mount them (for upright, inverted etc…), and finally how to image them with various microscopy techniques (from super resolution microscopies -liveSR or STED- to microscopies dedicated to thick samples (two-photon, ultramicroscope), from fast optical scanning spinning-disk to High Content Screening -HiTech and LowTech-).

Bench work is possible thanks to the TBM Core Facility, and the microscopes are provided by the BIC facility and the LP2N (home-made setup). 

This workshop is open to all (PhD students, Postdocs, Engineers, Technicians, Researchers and Teaching Assistants) who would like to learn how to prepare their 3D samples for photon microscopy.

The number of attendees will depend on the rules that will prevail at this time. Seats will be limited.

Registration is free but mandatory https://wkshpimageoids.sciencesconf.org/

Registration deadline: 6th Sept at noon. The organisation committee will then select participants and contact them shortly after registration closure.

There will be 4 seminars with the following keynote speakers:

  • Xavier Trepat –link– (IBEC, Barcelone)
  • Fanny Jaulin –link– (Institut Gustave Roussy, Paris)
  • Laura Broutier –link– (CRCL, Lyon)
  • Charlotte Rivière –link– (ILM, Lyon)


Each seminar will be preceded by a talk from our industry partners: Corning, StemCell, TreeFrog Therapeutics and Idylle. They will be accessible freely on a dedicated streaming platform, upon ad-hoc registration which will be set soon.

This workshop is organized with the support of the GdR ImaBio, the GdR Organoids and France Bio-Imaging.